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المؤلفون: Jennifer M. Bossert, Marco Venniro, Yavin Shaham, Hannah Korah, Olivia M. Lofaro, Sarah V. Applebey, David J. Reiner, Carlo Cifani
المصدر: J Neurosci
مصطلحات موضوعية: 0301 basic medicine, media_common.quotation_subject, Drug-Seeking Behavior, Prefrontal Cortex, Fentanyl, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Recurrence, medicine, Humans, Research Articles, media_common, business.industry, General Neuroscience, Abstinence, 030104 developmental biology, Baclofen, medicine.anatomical_structure, chemistry, Muscimol, Opioid, nervous system, Anesthesia, Orbitofrontal cortex, business, Self-administration, 030217 neurology & neurosurgery, psychological phenomena and processes, medicine.drug, Basolateral amygdala
الوصف: We recently developed a rat model of relapse to drug seeking after food choice-induced voluntary abstinence. Here, we used this model to study the role of the orbitofrontal cortex (OFC) and its afferent projections in relapse to fentanyl seeking. We trained male and female rats to self-administer palatable food pellets for 6 d (6 h/d) and intravenous fentanyl (2.5 μg/kg/infusion) for 12 d (6 h/d). We assessed relapse to fentanyl seeking after 13–14 voluntary abstinence days, achieved through a discrete choice procedure between fentanyl infusions and palatable food (20 trials/d). In both sexes, relapse after food choice-induced abstinence was associated with increased expression of the activity marker Fos in the OFC. Pharmacological inactivation of the OFC with muscimol plus baclofen (50 + 50 ng/side) decreased relapse to fentanyl seeking. We then determined projection-specific activation of OFC afferents during the relapse test by using Fos plus the retrograde tracer cholera toxin B (injected into the OFC). Relapse to fentanyl seeking was associated with increased Fos expression in the piriform cortex (Pir) neurons projecting to the OFC, but not in projections from the basolateral amygdala and thalamus. Pharmacological inactivation of the Pir with muscimol plus baclofen decreased relapse to fentanyl seeking after voluntary abstinence. Next, we used an anatomical disconnection procedure to determine whether projections between the Pir and OFC are critical for relapse to fentanyl seeking. Unilateral muscimol plus baclofen injections into the Pir in one hemisphere plus unilateral muscimol plus baclofen injections into the OFC in the contralateral, but not ipsilateral, hemisphere decreased relapse. Our results identify Pir–OFC projections as a new motivation-related pathway critical to relapse to opioid seeking after voluntary abstinence.SIGNIFICANCE STATEMENTThere are few preclinical studies of fentanyl relapse, and these studies have used experimenter-imposed extinction or forced abstinence procedures. In humans, however, abstinence is often voluntary, with drug available in the drug environment but forgone in favor of nondrug alternative reinforcers. We recently developed a rat model of drug relapse after palatable food choice-induced voluntary abstinence. Here, we used classical pharmacology, immunohistochemistry, and retrograde tracing to demonstrate a critical role of the piriform and orbitofrontal cortices in relapse to opioid seeking after voluntary abstinence.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89a735a3a982c01fafff9872bd62d8c9Test
https://europepmc.org/articles/PMC7083529Test/ -
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المؤلفون: Jennifer M. Bossert, David J. Reiner
المصدر: Neuropsychopharmacology. 43:1983-1984
مصطلحات موضوعية: 0301 basic medicine, media_common.quotation_subject, MEDLINE, Bioinformatics, Glucagon-Like Peptide-1 Receptor, Cocaine-Related Disorders, 03 medical and health sciences, 0302 clinical medicine, Glucagon-Like Peptide 1, Recurrence, Humans, Medicine, media_common, Pharmacology, business.industry, Addiction, Ventral Tegmental Area, Drug Repositioning, Research Highlight, Psychiatry and Mental health, Drug repositioning, 030104 developmental biology, Anti obesity, Anti-Obesity Agents, business, 030217 neurology & neurosurgery
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::160ecf152f0f8f9cab9d54bb72b30e12Test
https://doi.org/10.1038/s41386-018-0035-7Test