Sab is differentially expressed in the brain and affects neuronal activity
| العنوان: | Sab is differentially expressed in the brain and affects neuronal activity |
|---|---|
| المؤلفون: | Chiara Salio, Monica Rodriguez-Silva, Alejandro O. Sodero, Marco Sassoè-Pognetto, Jeremy W. Chambers |
| المصدر: | Brain Research. 2017;1670:76-85 Repositorio Institucional (UCA) Pontificia Universidad Católica Argentina instacron:UCA |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Scaffold protein, Cerebellum, Hippocampus, Hippocampal formation, Signal transduction, Mice, Premovement neuronal activity, NEURON, Neurons, QUINASA, PROTEINAS, Hippocampus, Mitogen-activated protein kinase, Neuron, Sab, Signal transduction, General Neuroscience, SIGNAL TRANSDUCTION, Brain, SAB, Mitochondria, Sab, Medicina Básica, medicine.anatomical_structure, Mitogen-activated protein kinase, CIENCIAS MÉDICAS Y DE LA SALUD, Inmunología, Biology, Mitochondrial Proteins, 03 medical and health sciences, CEREBRO, medicine, Animals, Molecular Biology, Adaptor Proteins, Signal Transducing, JNK Mitogen-Activated Protein Kinases, Membrane Proteins, MITOGEN-ACTIVATED PROTEIN KINASE, Dendrites, Neuron, 030104 developmental biology, nervous system, Gene Expression Regulation, Astrocytes, HIPPOCAMPUS, biology.protein, Neurology (clinical), ACTIVIDAD NEURONAL, Neuroscience, Developmental Biology |
| الوصف: | Fil: Sodero, Alejandro Omar. Florida International University. Herbert Wertheim College of Medicine. Department of Cellular Biology and Pharmacology; Estados Unidos Fil: Sodero, Alejandro Omar. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Sodero, Alejandro Omar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodriguez-Silva, Mónica. Florida International University. Herbert Wertheim College of Medicine. Department of Cellular Biology and Pharmacology; Estados Unidos Fil: Salio, Chiara. University of Turin. Department of Veterinary Sciences; Italia Fil: Sassoè-Pognetto, Marco. University of Turin. Department of Neuroscience; Italia Fil: Chambers, Jeremy W. Florida International University. Herbert Wertheim College of Medicine. Department of Cellular Biology and Pharmacology; Estados Unidos Fil: Chambers, Jeremy W. Florida International University. Herbert Wertheim College of Medicine. Department of Cellular Biology and Pharmacology; Estados Unidos Fil: Chambers, Jeremy W. Florida International University. Biomolecular Sciences Institute; Estados Unidos Abstract: Sab (SH3 binding protein 5 or SH3BP5) is a mitochondrial scaffold protein involved in signaling associated with mitochondrial dysfunction and apoptosis; furthermore, Sab is a crucial signaling platform for neurodegenerative disease. To determine how this signaling nexus could have a significant effect on disease, we examined the regional abundance of Sab in the brain and sub-neuronal distribution, and we monitored the effect of Sab-mediated signaling on neuronal activity. We found that Sab is widely expressed in the adult mouse brain with increased abundance in hippocampus, ventral midbrain, and cerebellum. Sab was found in purified synaptosomes and in cultures of hippocampal neurons and astrocytes. Confocal and electron microscopy of mouse hippocampal sections confirmed the mitochondrial localization of Sab in the soma, dendrites, and axons. Given the localization and sub-neuronal distribution of Sab, we postulated that Sab-mediated signaling could affect neuronal function, so we measured the impact of inhibiting Sab-mediated events on the spontaneous activity in cultured hippocampal neurons. Treatment with a Sab-inhibitory peptide (Tat-SabKIM1), but not a scrambled control peptide, decreased the firing frequency and spike amplitudes. Our results demonstrate that brain-specific Sab-mediated signaling plays a role in neuronal activity through the manipulation of mitochondrial physiology by interacting kinases. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b09747d9abe02c5bde3a3b2326f61919Test http://hdl.handle.net/2318/1647281Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b09747d9abe02c5bde3a3b2326f61919 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |