Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-β-Lactamase
| العنوان: | Molecular Characterization of Carbapenemase-Producing Pseudomonas aeruginosa of Czech Origin and Evidence for Clonal Spread of Extensively Resistant Sequence Type 357 Expressing IMP-7 Metallo-β-Lactamase |
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| المؤلفون: | Costas C, Papagiannitsis, Matej, Medvecky, Katerina, Chudejova, Anna, Skalova, Veronika, Rotova, Petra, Spanelova, Vladislav, Jakubu, Helena, Zemlickova, Jaroslav, Hrabak, R, Zaruba |
| المصدر: | Antimicrobial Agents and Chemotherapy. 61 |
| بيانات النشر: | American Society for Microbiology, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Transposable element, Genomic Islands, Genotype, 030106 microbiology, Gene Expression, Microbial Sensitivity Tests, medicine.disease_cause, Integron, beta-Lactamases, Integrons, Microbiology, 03 medical and health sciences, Mechanisms of Resistance, Drug Resistance, Multiple, Bacterial, Genomic island, medicine, Humans, Pseudomonas Infections, Pharmacology (medical), Gene, Czech Republic, Pharmacology, biology, Pseudomonas aeruginosa, Incidence, Chromosome, biochemical phenomena, metabolism, and nutrition, Chromosomes, Bacterial, bacterial infections and mycoses, Hospitals, Anti-Bacterial Agents, Isoenzymes, Infectious Diseases, Carbapenems, Epidemiological Monitoring, biology.protein, bacteria, Multilocus sequence typing, Multilocus Sequence Typing |
| الوصف: | The objective of this study was to perform molecular surveillance for assessing the spread of carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six carbapenemase-producing isolates were recovered from 22 hospitals located throughout the country. Sequence type 357 (ST357) dominated ( n = 120) among carbapenemase producers. One hundred seventeen isolates produced IMP-type (IMP-7 [ n = 116] and IMP-1 [ n = 1]) metallo-β-lactamases (MβLs), 15 produced the VIM-2 MβL, and the remaining isolates expressed the GES-5 enzyme. The bla IMP -like genes were located in three main integron types, with In-p110-like being the most prevalent ( n = 115). The two other IMP-encoding integrons (In1392 and In1393) have not been described previously. bla VIM-2 -carrying integrons included In59-like, In56, and a novel element (In1391). bla GES-5 was carried by In717. Sequencing data showed that In-p110-like was associated with a Tn 4380 -like transposon inserted in genomic island LESGI-3 in the P. aeruginosa chromosome. The other integrons were also integrated into the P. aeruginosa chromosome. These findings indicated the clonal spread of ST357 P. aeruginosa , carrying the IMP-7-encoding integron In-p110, in Czech hospitals. Additionally, the sporadic emergence of P. aeruginosa producing different carbapenemase types, associated with divergent or novel integrons, punctuated the ongoing evolution of these bacteria. |
| تدمد: | 1098-6596 0066-4804 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67cb35cf42bde88a5ef4e918686bec2aTest https://doi.org/10.1128/aac.01811-17Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67cb35cf42bde88a5ef4e918686bec2a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10986596 00664804 |
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