Clostridioides difficile exploits toxin-mediated inflammation to alter the host nutritional landscape and exclude competitors from the gut microbiota
| العنوان: | Clostridioides difficile exploits toxin-mediated inflammation to alter the host nutritional landscape and exclude competitors from the gut microbiota |
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| المؤلفون: | Colleen M. Pike, Matthew H. Foley, Stephanie A. Montgomery, Casey M. Theriot, Alissa J. Rivera, Ruth J. Parsons, Michael R. McLaren, Joshua R. Fletcher |
| المصدر: | Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Antibiotics, General Physics and Astronomy, Gut flora, medicine.disease_cause, Mice, Sigma factor, Bacteroides, RNA-Seq, Intestinal Mucosa, Pathogen, Regulation of gene expression, Multidisciplinary, Anti-Bacterial Agents, Extracellular Matrix, RNA, Bacterial, Host-Pathogen Interactions, Female, Pathogens, medicine.symptom, medicine.drug_class, Science, Bacterial Toxins, 030106 microbiology, Sigma Factor, Inflammation, Colonisation resistance, Biology, digestive system, Article, General Biochemistry, Genetics and Molecular Biology, Bacterial genetics, Microbiology, 03 medical and health sciences, Bacterial Proteins, medicine, Animals, Humans, Colonic diseases, Toxin, Clostridioides difficile, Gene Expression Regulation, Bacterial, Nutrients, General Chemistry, Pseudomembranous colitis, biology.organism_classification, Matrix Metalloproteinases, Gastrointestinal Microbiome, Disease Models, Animal, 030104 developmental biology, Proteolysis, Clostridium Infections, Microbiome, Transcriptome |
| الوصف: | Clostridioides difficile is a bacterial pathogen that causes a range of clinical disease from mild to moderate diarrhea, pseudomembranous colitis, and toxic megacolon. Typically, C. difficile infections (CDIs) occur after antibiotic treatment, which alters the gut microbiota, decreasing colonization resistance against C. difficile. Disease is mediated by two large toxins and the expression of their genes is induced upon nutrient depletion via the alternative sigma factor TcdR. Here, we use tcdR mutants in two strains of C. difficile and omics to investigate how toxin-induced inflammation alters C. difficile metabolism, tissue gene expression and the gut microbiota, and to determine how inflammation by the host may be beneficial to C. difficile. We show that C. difficile metabolism is significantly different in the face of inflammation, with changes in many carbohydrate and amino acid uptake and utilization pathways. Host gene expression signatures suggest that degradation of collagen and other components of the extracellular matrix by matrix metalloproteinases is a major source of peptides and amino acids that supports C. difficile growth in vivo. Lastly, the inflammation induced by C. difficile toxin activity alters the gut microbiota, excluding members from the genus Bacteroides that are able to utilize the same essential nutrients released from collagen degradation. The effects of antibiotics on the gut microbiota can lead to enhanced colonization of Clostridioides difficile (C. difficile) and toxin-mediated pathogenesis. Here, using defined toxin-mutant strains and a murine model, the authors provide insights into how toxin-induced inflammation alters C. difficile metabolism, host tissue gene expression and gut microbiota, together influencing a beneficial niche for infection. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27f1dced314a70d0ed372007695ffe64Test https://doaj.org/article/9e6aa7aed98f489781f33be6a4d04611Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....27f1dced314a70d0ed372007695ffe64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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