Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Japanese Cattle
| العنوان: | Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Japanese Cattle |
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| المؤلفون: | Atsushi Iguchi, Kyohei Fujiwara, Jun Ogasawara, Atsushi Fujiwara, Hiromi Nakamura, Tomofumi Maehara |
| المصدر: | Japanese Journal of Infectious Diseases. 71:45-50 |
| بيانات النشر: | Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Serotype, Genetics, 030106 microbiology, General Medicine, biochemical phenomena, metabolism, and nutrition, Multiple Loci VNTR Analysis, Biology, bacterial infections and mycoses, medicine.disease_cause, Subtyping, 03 medical and health sciences, fluids and secretions, 030104 developmental biology, Infectious Diseases, Tandem repeat, medicine, Pulsed-field gel electrophoresis, bacteria, Multilocus sequence typing, Typing, Escherichia coli |
| الوصف: | To determine the infection source, route, and extent of an outbreak, it is important to subtype Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the same serotype for clustering into clonally related groups. In this study, we compared 3 molecular subtyping methods-multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and multiple-locus variable-number of tandem repeat analysis (MLVA)-using O157 and non-O157 STEC isolates from Japanese beef cattle. A total of 73 STEC isolates belonging to 9 O-serogroups were analyzed. By means of 3 molecular subtyping methods, the strains were subdivided into 9 MLST sequence types (STs), 23 PFGE types, and 26 MLVA types. The STEC classification by O-serogrouping and MLST was almost identical. Furthermore, PFGE and MLVA could systematically classify STEC isolates of the same serotypes and STs. MLVA and PFGE were found to be highly efficient subtyping methods after O-serogrouping for the classification of not only O157 but also non-O157 STEC isolates in an outbreak investigation. |
| تدمد: | 1884-2836 1344-6304 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::b00223df73694fef5b112ea25cec7697Test https://doi.org/10.7883/yoken.jjid.2017.297Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........b00223df73694fef5b112ea25cec7697 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18842836 13446304 |
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