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المؤلفون: Masataka Sakane, Hirotaka Mutsuzaki, Hiromi Nakajima
المصدر: Journal of Physical Therapy Science
مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, business.industry, Anterior cruciate ligament, Physical Therapy, Sports Therapy and Rehabilitation, Articular cartilage, musculoskeletal system, Anterior cruciate ligament insertion, Remobilization, Chondrocyte, Surgery, 03 medical and health sciences, Joint disease, 030104 developmental biology, medicine.anatomical_structure, Proliferation rate, Time course, medicine, JAPANESE WHITE, Ligament, Original Article, business
الوصف: [Purpose] To investigate the influence of knee immobilization period on recovery of histological damages in the anterior cruciate ligament (ACL) insertion and articular cartilage in rabbits. This knowledge is important for determining the appropriate rehabilitation approach for patients with ligament injuries, fracture, disuse atrophy, and degenerative joint disease. [Materials and Methods] Forty-eight male Japanese white rabbits were divided equally into the remobilization and control groups. The remobilization group had the right knee surgically immobilized, and was divided equally into four subgroups according to the duration of immobilization (1, 2, 4 and 8 weeks). After the immobilization was removed, the rabbits moved freely for 8 weeks. The control group underwent sham operation and followed the same time course as the remobilization group. The chondrocyte apoptosis rate and chondrocyte proliferation rate in the ACL insertion and articular cartilage were analyzed after remobilization. [Results] In the ACL insertion, the remobilization group had a higher chondrocyte apoptosis rate than the control group after 8 weeks of immobilization, and a lower chondrocyte proliferation rate than the control group after 4 and 8 weeks of immobilization. In the articular cartilage, the remobilization group had a lower chondrocyte proliferation rate than the control group after 8 weeks of immobilization. After 8 weeks of remobilization, the ACL insertion and articular cartilage are not completely recovered after 4 and 8 weeks of immobilization, respectively. [Conclusion] Our results suggest that 8 weeks of remobilization will result in recovery of the ACL insertion after 2 weeks of knee immobilization, and recovery of the articular cartilage after 4 weeks of knee immobilization. If 8 weeks of immobilization occurs, a remobilization duration of more than 8 weeks may be necessary.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d9cc80dd58706b6b28a84d84d9c7757Test
https://doi.org/10.1589/jpts.30.140Test -
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المصدر: Current Oncology
Volume 23
Issue 4
Pages 3137-430مصطلحات موضوعية: medicine.medical_specialty, medicine.medical_treatment, autologous stem-cell transplantation, 03 medical and health sciences, 0302 clinical medicine, Autologous stem-cell transplantation, collection, medicine, Intensive care medicine, mobilization, Multiple myeloma, Chemotherapy, Mobilization, business.industry, non-Hodgkin lymphoma, Plerixafor, Guideline, medicine.disease, Transplantation, remobilization, Regimen, Practice Guideline, 030220 oncology & carcinogenesis, CD34+ cell count, business, Hodgkin lymphoma, 030215 immunology, medicine.drug
الوصف: High-dose chemotherapy with autologous stem-cell transplantation (asct) is an accepted part of standard therapy for patients with hematologic malignancies. Usually, stem-cell mobilization uses granulocyte colony&ndash
stimulating factor (g-csf)
however, some patients are not able to be mobilized with chemotherapy and g-csf, and such patients could be at higher risk of failing mobilization. Plerixafor is a novel mobilization agent that is absorbed quickly after subcutaneous injection and, at the recommended dose of 0.24 mg/kg, provides a sustained increase in circulating CD34+ cells for 10&ndash
18 hours. The main purpose of the present report was to evaluate the most current evidence on the efficacy of plerixafor in enhancing hematopoietic stem-cell mobilization and collection before asct for patients in Ontario so as to make recommendations for clinical practice and to assist Cancer Care Ontario in decision-making with respect to this intervention. The medline and embase databases were systematically searched for evidence from January 1996 to March 2015, and the best available evidence was used to draft recommendations relevant to the efficacy of plerixafor in enhancing hematopoietic stem-cell mobilization and collection before asct. Final approval of this practice guideline report was obtained from both the Stem Cell Transplant Steering Committee and the Report Approval Panel of the Program in Evidence-Based Care. These recommendations apply to adult patients considered for asct: ■
Adding plerixafor to g-csf is an option for initial mobilization in patients with non-Hodgkin lymphoma or multiple myeloma who are eligible for asct when chemotherapy cannot be used and only g-csf mobilization is available. ■
For patients with a low peripheral blood CD34+ cell count (for example
L) at the time of anticipated stem-cell harvesting, or with an inadequate first-day apheresis collection, it is recommended that plerixafor be added to the mobilization regimen to maximize stem-cell collection and to prevent the need for remobilization. ■
It is recommended that patients who have failed a previous mobilization attempt undergo remobilization with g-csf and plerixafor, with or without chemotherapy.وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8e4483ec44d9bbde5e58e10b071d44bTest
https://doi.org/10.3747/co.23.3137Test -
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المؤلفون: Gabriella Sonnante, Diana L. Zuluaga
المصدر: Plants 8(8), 294 (2019).
info:cnr-pdr/source/autori:Diana L. Zuluaga, Gabriella Sonnante/titolo:The Use of Nitrogen and Its Regulation in Cereals: Structural Genes, Transcription Factors, and the Role of miRNAs/doi:/rivista:Plants/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:8(8), 294
Plants, Vol 8, Iss 8, p 294 (2019)
Plantsمصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Nitrogen assimilation, Population, Gene regulatory network, Plant Science, Review, Biology, 01 natural sciences, 03 medical and health sciences, nitrogen use, education, Transcription factor, Nitrogen cycle, Ecology, Evolution, Behavior and Systematics, nitrogen transport, miRNA, cereals, education.field_of_study, Ecology, Abiotic stress, business.industry, Structural gene, Botany, food and beverages, Assimilation (biology), nitrogen assimilation, nitrogen remobilization, target genes, Biotechnology, 030104 developmental biology, QK1-989, business, 010606 plant biology & botany
الوصف: Cereals and, especially, rice, maize, and wheat, are essential commodities, on which human nutrition is based. Expanding population and food demand have required higher production which has been achieved by increasing fertilization, and especially nitrogen supply to cereal crops. In fact, nitrogen is a crucial nutrient for the plant, but excessive use poses serious environmental and health issues. Therefore, increasing nitrogen use efficiency in cereals is of pivotal importance for sustainable agriculture. The main steps in the use of nitrogen are uptake and transport, reduction and assimilation, and translocation and remobilization. Many studies have been carried out on the genes involved in these phases, and on transcription factors regulating these genes. Lately, increasing attention has been paid to miRNAs responding to abiotic stress, including nutrient deficiency. Many miRNAs have been found to regulate transcription factors acting on the expression of specific genes for nitrogen uptake or remobilization. Recent studies on gene regulatory networks have also demonstrated that miRNAs can interact with several nodes in the network, functioning as key regulators in nitrogen metabolism.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fe7a554f6b56f6da67552237b1fa3aeTest
http://www.cnr.it/prodotto/i/406366Test -
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المؤلفون: Tomoki Aoyama, Xiangkai Zhang, Shoki Yamaguchi, Momoko Nagai, Junichi Tajino, Hirotaka Iijima, Hiroshi Kuroki, Akira Ito
المصدر: Journal of Anatomy
مصطلحات موضوعية: 0301 basic medicine, musculoskeletal diseases, Cartilage, Articular, Male, Histology, degeneration, Cell Count, Degeneration (medical), Knee Joint, 03 medical and health sciences, Immobilization, 0302 clinical medicine, Chondrocytes, medicine, aggravation, Animals, Cyst, Femur, Tibia, Rats, Wistar, cartilage, Molecular Biology, Collagen Type II, Ecology, Evolution, Behavior and Systematics, 030203 arthritis & rheumatology, cyst, business.industry, Cysts, Cartilage, Cell Biology, Anatomy, Original Articles, medicine.disease, musculoskeletal system, Rats, remobilization, 030104 developmental biology, medicine.anatomical_structure, Hyaluronan Receptors, Cyst formation, Transitional Region, Original Article, business, Cartilage Diseases, Developmental Biology
الوصف: An understanding of the articular cartilage degenerative process is necessary for the prevention and treatment of joint disease. The present study aimed to examine how long‐term immobilization‐induced cartilage degeneration is aggravated by remobilization. Sixty 8‐week‐old male Wistar rats were used in this study. The unilateral knee joint was immobilized using an external fixator for 8 weeks. The rats were killed at 0 and 3 days, and at 1, 2, 4 and 8 weeks after removing the fixator. After the rats were killed, the maximum knee extension angles were measured. Histological sections at the medial mid‐condylar region (non‐contact, transitional and contact regions of the femur and tibia) were prepared and scored. The cartilage thickness and number of chondrocytes were measured, and CD44 and Col2‐3/4c expression levels were assessed immunohistochemically. The histological assessment revealed progressive aggravation of cartilage degeneration in the transitional region, with a decreased number of chondrocytes and CD44‐positive chondrocytes as well as poor scoring over time, particularly in the tibia. Cyst formation was confirmed in the transitional region of the tibia at 8 weeks post‐remobilization. The cartilage thickness in the transitional region was thicker than that in the contact region, particularly in the tibia. Col2‐3/4c expression was observed in the non‐contact and transitional regions, and the knee extension angle was recovered. In conclusion, immobilization‐induced cartilage degeneration was aggravated by remobilization over time in the transitional region, followed by observations of a decreased number of chondrocytes and morphological disparity between different cartilage regions.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81559a35ac0d4fac23e3bdbab4921a6eTest
http://europepmc.org/articles/PMC5341580Test -
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المؤلفون: Fabien Chardon, Mathieu Pottier, Michèle Reisdorf-Cren, Daiki Shinozaki, Sébastien Thomine, Kohki Yoshimoto, Céline Masclaux-Daubresse, Marien Havé, Jie Luo, Anne Marmagne, Qinwu Chen
المصدر: Cells
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Nutrient cycle, leaf senescence, Plant Development, Review, Biology, 01 natural sciences, nitrogen use efficiency, 03 medical and health sciences, iron, Nutrient, Stress, Physiological, Autophagy, Plant Proteins, 2. Zero hunger, plant proteases, business.industry, zinc, fungi, food and beverages, nitrogen remobilization, Nutrients, General Medicine, Plant engineering, Plants, 15. Life on land, Biotechnology, 030104 developmental biology, Metabolic Engineering, Seeds, business, 010606 plant biology & botany
الوصف: Nutrient recycling and mobilization from organ to organ all along the plant lifespan is essential for plant survival under changing environments. Nutrient remobilization to the seeds is also essential for good seed production. In this review, we summarize the recent advances made to understand how plants manage nutrient remobilization from senescing organs to sink tissues and what is the contribution of autophagy in this process. Plant engineering manipulating autophagy for better yield and plant tolerance to stresses will be presented.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::709d39175f86927b4c59d68b103f892aTest
https://doi.org/10.3390/cells8111426Test -
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المؤلفون: Ben Berkhout
المساهمون: AII - Infectious diseases, Medical Microbiology and Infection Prevention, AII - Amsterdam institute for Infection and Immunity
المصدر: Molecular therapy, 25(8), 1741-1743. Nature Publishing Group
Molecular Therapyمصطلحات موضوعية: 0301 basic medicine, Gymnastics, HIV-1 remobilization, Computer science, Genetic enhancement, Transgene, Genetic Vectors, Gene transfer, Computational biology, Genome, Viral vector, splicing, 03 medical and health sciences, HIV-1 packaging, Transduction, Genetic, hemophilia, Drug Discovery, Genetics, Fourth generation, Vector (molecular biology), Stable gene, Molecular Biology, Pharmacology, business.industry, Lentivirus, viral vector, Genomics, reverse transcription, gene therapy, Biotechnology, 030104 developmental biology, Molecular Medicine, Original Article, business
الوصف: Lentiviral vector genomic RNA requires sequences that partially overlap wild-type HIV-1 gag and env genes for packaging into vector particles. These HIV-1 packaging sequences constitute 19.6% of the wild-type HIV-1 genome and contain functional cis elements that potentially compromise clinical safety. Here, we describe the development of a novel lentiviral vector (LTR1) with a unique genomic structure designed to prevent transfer of HIV-1 packaging sequences to patient cells, thus reducing the total HIV-1 content to just 4.8% of the wild-type genome. This has been achieved by reconfiguring the vector to mediate reverse-transcription with a single strand transfer, instead of the usual two, and in which HIV-1 packaging sequences are not copied. We show that LTR1 vectors offer improved safety in their resistance to remobilization in HIV-1 particles and reduced frequency of splicing into human genes. Following intravenous luciferase vector administration to neonatal mice, LTR1 sustained a higher level of liver transgene expression than an equivalent dose of a standard lentivirus. LTR1 vectors produce reverse-transcription products earlier and start to express transgenes significantly quicker than standard lentiviruses after transduction. Finally, we show that LTR1 is an effective lentiviral gene therapy vector as demonstrated by correction of a mouse hemophilia B model.
Counsell and colleagues have developed a novel lentiviral vector, LTR1, designed to minimize the incorporation of HIV-1 sequences during transduction. Their data show that LTR1 potentially offers improved safety in gene therapy, while demonstrating robust gene therapy efficacy in correcting a mouse model of hemophilia B.الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a1a387d025d2fff4e846da5df11aab8Test
https://pure.amc.nl/en/publications/a-fourth-generation-lentiviral-vector-simplifying-genomic-gymnasticsTest(e1644c70-e92c-4435-98ec-7aae7091e343).html -
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المساهمون: Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Centre de Recherche de Chappes, BIOGEMMA
المصدر: TAG Theoretical and Applied Genetics
TAG Theoretical and Applied Genetics, Springer Verlag, 2014, 127 (12), pp.2679-2693. ⟨10.1007/s00122-014-2407-7⟩
Theoretical and Applied Geneticsمصطلحات موضوعية: 0106 biological sciences, Candidate gene, Linkage disequilibrium, LINKAGE DISEQUILIBRIUM, [SDV]Life Sciences [q-bio], 01 natural sciences, Genome, F30 - Génétique et amélioration des plantes, CONFIDENCE-INTERVALS, Gene Frequency, Triticum, 2. Zero hunger, 0303 health sciences, Chromosome Mapping, General Medicine, WINTER-WHEAT, GRAIN PROTEIN, MAPPING QTLS, Phenotype, [SDE]Environmental Sciences, Trait, Biotechnology, Genotype, Nitrogen, F60 - Physiologie et biochimie végétale, NUTRIENT REMOBILIZATION, Biology, Polymorphism, Single Nucleotide, 03 medical and health sciences, Quantitative Trait, Heritable, PLANT HEIGHT, Genetic variation, Genetics, SNP, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, POPULATION-STRUCTURE, Fertilizers, Allele frequency, Selection (genetic algorithm), Genetic Association Studies, 030304 developmental biology, Models, Genetic, business.industry, ASSOCIATION ANALYSIS, business, Agronomy and Crop Science, HEXAPLOWHEAT, 010606 plant biology & botany, F04 - Fertilisation
الوصف: International audience; This study identified 333 genomic regions associated to 28 traits related to nitrogen use efficiency in European winter wheat using genome-wide association in a 214-varieties panel experimented in eight environments. Improving nitrogen use efficiency is a key factor to sustainably ensure global production increase. However, while high-throughput screening methods remain at a developmental stage, genetic progress may be mainly driven by marker-assisted selection. The objective of this study was to identify chromosomal regions associated with nitrogen use efficiency-related traits in bread wheat (Triticum aestivum L.) using a genome-wide association approach. Two hundred and fourteen European elite varieties were characterised for 28 traits related to nitrogen use efficiency in eight environments in which two different nitrogen fertilisation levels were tested. The genome-wide association study was carried out using 23,603 SNP with a mixed model for taking into account parentage relationships among varieties. We identified 1,010 significantly associated SNP which defined 333 chromosomal regions associated with at least one trait and found colocalisations for 39 % of these chromosomal regions. A method based on linkage disequilibrium to define the associated region was suggested and discussed with reference to false positive rate. Through a network approach, colocalisations were analysed and highlighted the impact of genomic regions controlling nitrogen status at flowering, precocity, and nitrogen utilisation on global agronomic performance. We were able to explain 40 +/- A 10 % of the total genetic variation. Numerous colocalisations with previously published genomic regions were observed with such candidate genes as Ppd-D1, Rht-D1, NADH-Gogat, and GSe. We highlighted selection pressure on yield and nitrogen utilisation discussing allele frequencies in associated regions.
وصف الملف: text
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00a81882f10df416435320468ea32d2fTest
https://hal.inrae.fr/hal-02635490Test -
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المؤلفون: Céline Masclaux-Daubresse, Julie Dechorgnat, Françoise Daniel-Vedele, Fabien Chardon, Laure Gaufichon, Akira Suzuki
المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech
المصدر: Annals of Botany
Annals of Botany, Oxford University Press (OUP), 2010, 105 (7), pp.1141-1157. ⟨10.1093/aob/mcq028⟩مصطلحات موضوعية: 0106 biological sciences, nutrient recycling, Nutrient cycle, variation naturelle, Nitrogen, leaf senescence, nitrate transporter, chemistry.chemical_element, Plant Science, Biology, 01 natural sciences, Crop, 03 medical and health sciences, chemistry.chemical_compound, Nitrate, Ammonium, natural variation, ammonium assimilation, 030304 developmental biology, Plant Proteins, 2. Zero hunger, 0303 health sciences, Nitrogen use efficiency, business.industry, fungi, food and beverages, glutamine synthetase, Assimilation (biology), Articles, 15. Life on land, Plants, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, remobilization, trait caractéristique, Agronomy, chemistry, Agriculture, quantitative trait loci, business, Plant nutrition, pousse sénescence, 010606 plant biology & botany
الوصف: Productive agriculture needs a large amount of expensive nitrogenous fertilizers. Improving nitrogen use efficiency (NUE) of crop plants is thus of key importance. NUE definitions differ depending on whether plants are cultivated to produce biomass or grain yields. However, for most plant species, NUE mainly depends on how plants extract inorganic nitrogen from the soil, assimilate nitrate and ammonium, and recycle organic nitrogen. Efforts have been made to study the genetic basis as well as the biochemical and enzymatic mechanisms involved in nitrogen uptake, assimilation, and remobilization in crops and model plants. The detection of the limiting factors that could be manipulated to increase NUE is the major goal of such research. An overall examination of the physiological, metabolic, and genetic aspects of nitrogen uptake, assimilation and remobilization is presented in this review. The enzymes and regulatory processes manipulated to improve NUE components are presented. Results obtained from natural variation and quantitative trait loci studies are also discussed. This review presents the complexity of NUE and supports the idea that the integration of the numerous data coming from transcriptome studies, functional genomics, quantitative genetics, ecophysiology and soil science into explanatory models of whole-plant behaviour will be promising.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb411a1d4dc94ef157d06abaf5914cd1Test
https://hal.archives-ouvertes.fr/hal-01203920Test
