Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes
| العنوان: | Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes |
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| المؤلفون: | Linda J. Harris, Aida Z. Kebede, Whynn Bosnich, Anne Johnston, Danielle Schneiderman |
| المصدر: | BMC Genomics, Vol 19, Iss 1, Pp 1-12 (2018) BMC Genomics |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Candidate gene, lcsh:QH426-470, Gibberella, QTL, lcsh:Biotechnology, Quantitative Trait Loci, Fungal pathogen, RNA-Seq, Quantitative trait locus, Plant disease resistance, Genes, Plant, Zea mays, Transcriptome, 03 medical and health sciences, Fusarium, Species Specificity, Gene Expression Regulation, Plant, lcsh:TP248.13-248.65, Genetics, Inbreeding, Cell wall modification, Gene, Plant Diseases, Gibberella ear rot, Disease resistance, biology, High-Throughput Nucleotide Sequencing, food and beverages, biology.organism_classification, Maize, Fusarium graminearum, lcsh:Genetics, Gene Ontology, 030104 developmental biology, Host-Pathogen Interactions, Gene expression, Research Article, Biotechnology |
| الوصف: | Background Gibberella ear rot (GER) is one of the most economically important fungal diseases of maize in the temperate zone due to moldy grain contaminated with health threatening mycotoxins. To develop resistant genotypes and control the disease, understanding the host-pathogen interaction is essential. Results RNA-Seq-derived transcriptome profiles of fungal- and mock-inoculated developing kernel tissues of two maize inbred lines were used to identify differentially expressed transcripts and propose candidate genes mapping within GER resistance quantitative trait loci (QTL). A total of 1255 transcripts were significantly (P ≤ 0.05) up regulated due to fungal infection in both susceptible and resistant inbreds. A greater number of transcripts were up regulated in the former (1174) than the latter (497) and increased as the infection progressed from 1 to 2 days after inoculation. Focusing on differentially expressed genes located within QTL regions for GER resistance, we identified 81 genes involved in membrane transport, hormone regulation, cell wall modification, cell detoxification, and biosynthesis of pathogenesis related proteins and phytoalexins as candidate genes contributing to resistance. Applying droplet digital PCR, we validated the expression profiles of a subset of these candidate genes from QTL regions contributed by the resistant inbred on chromosomes 1, 2 and 9. Conclusion By screening global gene expression profiles for differentially expressed genes mapping within resistance QTL regions, we have identified candidate genes for gibberella ear rot resistance on several maize chromosomes which could potentially lead to a better understanding of Fusarium resistance mechanisms. Electronic supplementary material The online version of this article (10.1186/s12864-018-4513-4) contains supplementary material, which is available to authorized users. |
| تدمد: | 1471-2164 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26aee98481e97a52bfde12a9ed171900Test https://doi.org/10.1186/s12864-018-4513-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....26aee98481e97a52bfde12a9ed171900 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712164 |
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