المؤلفون: David Kuo, Kiran Bandi, Lisa Long, Yaron Shoham, Divya Manoharan, Danyang Ma, Mahmoud A. Ghannoum, Eckhard Jankowsky, Michael Greenberg, Menachem Shoham, Chris Hager, William E. Harte

المصدر: Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports

مصطلحات موضوعية: 0301 basic medicine, medicine.drug_class, Virulence Factors, medicine.medical_treatment, 030106 microbiology, Antibiotics, lcsh:Medicine, Biology, medicine.disease_cause, Gram-Positive Bacteria, Article, Microbiology, Sepsis, 03 medical and health sciences, Mice, Bacterial Proteins, In vivo, medicine, Animals, lcsh:Science, Transcription factor, Multidisciplinary, Virulence, Toxin, lcsh:R, Drug Synergism, Staphylococcal Infections, biology.organism_classification, medicine.disease, Survival Analysis, In vitro, Anti-Bacterial Agents, Disease Models, Animal, 030104 developmental biology, Treatment Outcome, Trans-Activators, Wound Infection, lcsh:Q, Adjuvant, Bacteria

الوصف: Small-molecule antivirulence agents represent a promising alternative or adjuvant to antibiotics. These compounds disarm pathogens of disease-causing toxins without killing them, thereby diminishing survival pressure to develop resistance. Here we show that the small-molecule antivirulence agents F12 and F19 block staphylococcal transcription factor AgrA from binding to its promoter. Consequently, toxin expression is inhibited, thus preventing host cell damage by Gram-positive pathogens. Broad spectrum efficacy against Gram-positive pathogens is due to the existence of AgrA homologs in many Gram-positive bacteria. F12 is more efficacious in vitro and F19 works better in vivo. In a murine MRSA bacteremia/sepsis model, F19 treatment alone resulted in 100% survival while untreated animals had 70% mortality. Furthermore, F19 enhances antibiotic efficacy in vivo. Notably, in a murine MRSA wound infection model, combination of F19 with antibiotics resulted in bacterial load reduction. Thus, F19 could be used alone or in combination with antibiotics to prevent and treat infections of Gram-positive pathogens.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de77e4ef0eb87a8e3c66235e5cbbbdf1Test
http://link.springer.com/article/10.1038/s41598-018-32829-wTest

المؤلفون: Blake R. Gibson, Grant R. Whitmer, Sean T. Weaver, Hossein Heidari-Torkabadi, Jordan F. Gibson, Michael Greenberg, Marianne Pusztai-Carey, Paul R. Carey

المصدر: Biochemistry. 56:2247-2250

مصطلحات موضوعية: Protein Conformation, alpha-Helical, 0301 basic medicine, Aluminum foil, Growth phase, Carbohydrates, Analytical chemistry, Spectrum Analysis, Raman, medicine.disease_cause, Biochemistry, 03 medical and health sciences, symbols.namesake, Bacterial Proteins, Staphylococcus epidermidis, medicine, Escherichia coli, Escherichia coli K12, 030102 biochemistry & molecular biology, biology, Chemistry, Biofilm, Analytic Sample Preparation Methods, Reproducibility of Results, RNA, biochemical phenomena, metabolism, and nutrition, Plankton, biology.organism_classification, Raman microspectroscopy, RNA, Bacterial, Freeze Drying, 030104 developmental biology, Biofilms, symbols, Biophysics, Microtechnology, Protein Conformation, beta-Strand, Raman spectroscopy

الوصف: Two protocols that allow for the comparison of Raman spectra of planktonic cells and biofilm formed from these cells in their growth phase have been developed. Planktonic cells are washed and flash-frozen in

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e01205f6d33883039016cc86122c10d7Test
https://doi.org/10.1021/acs.biochem.7b00116Test

المؤلفون: Peter Aaby, Ofer Levy, Jacques Louis, Frank Shann, Michael Greenberg, William H. Warren, Mihai G. Netea, Mitra Saadatian-Elahi, Valentina Picot

المصدر: Vaccine, 34, 34, pp. 3923-30
Vaccine, 34, 3923-30

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Systems biology, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], Biology, Immunity, Heterologous, Vaccines, Attenuated, 03 medical and health sciences, medicine, Humans, Regulatory science, Attenuated vaccine, Heterologous vaccine, General Veterinary, General Immunology and Microbiology, Public health, Public Health, Environmental and Occupational Health, Congresses as Topic, Expert group, Immunity, Innate, Vaccination, 030104 developmental biology, Infectious Diseases, Vaccines, Inactivated, Immunology, Molecular Medicine, Engineering ethics, France, Immunologic Memory

الوصف: Item does not contain fulltext The heterologous or non-specific effects (NSEs) of vaccines, at times defined as "off-target effects" suggest that they can affect the immune response to organisms other than their pathogen-specific intended purpose. These NSEs have been the subject of clinical, immunological and epidemiological studies and are increasingly recognized as an important biological process by a growing group of immunologists and epidemiologists. Much remain to be learned about the extent and underlying mechanisms for these effects. The conference "Off-target effects of vaccination" held in Annecy-France (June 8-10 2015) intended to take a holistic approach drawing from the fields of immunology, systems biology, epidemiology, bioinformatics, public health and regulatory science to address fundamental questions of immunological mechanisms, as well as translational questions about vaccines NSEs. NSE observations were examined using case-studies on live attenuated vaccines and non-live vaccines followed by discussion of studies of possible biological mechanisms. Some possible pathways forward in the study of vaccines NSE were identified and discussed by the expert group.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f779dc2c6185c859964d348e7a82185Test
https://doi.org/10.1016/j.vaccine.2016.06.020Test

المؤلفون: Peter Richmond, Michael D. Nissen, Peter Howard, James M. McCaw, Michael H. Lai, Jodie McVernon, Terry Nolan, Theo P. Sloots, Michael Greenberg, Stephen B. Lambert

المصدر: Influenza and Other Respiratory Viruses

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Adult, Male, medicine.medical_specialty, Adolescent, Influenza vaccine, Cost effectiveness, Epidemiology, respiratory tract infection, Population, Orthomyxoviridae, Part 1, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Human metapneumovirus, Internal medicine, Influenza, Human, medicine, Humans, 030212 general & internal medicine, human, education, 030304 developmental biology, 0303 health sciences, Influenza-like illness, education.field_of_study, biology, business.industry, Public Health, Environmental and Occupational Health, Vaccine trial, virus diseases, Original Articles, Middle Aged, 16. Peace & justice, biology.organism_classification, 3. Good health, Vaccination, Infectious Diseases, Influenza Vaccines, Immunology, Viruses, Original Article, Female, picornavirus infection, business, influenza

الوصف: Background Viral respiratory infections are associated with significant morbidity and mortality. Many new aetiological agents have been described recently. Objectives We looked for respiratory viruses in a population-based sample of healthy adults with influenza-like illness (ILI). We investigated host and spatio-temporal associations with virus isolation and host, spatio-temporal and virus associations with self-reported symptoms. Patients/Methods We recruited 586 participants experiencing 651 illness episodes from a population of healthy adults enrolled in an influenza vaccine effectiveness trial. At ILI assessment visits, a respiratory swab was collected and tested for viruses using a combination of polymerase chain reaction (PCR) assays. Participants also completed a questionnaire detailing their clinical course in 336 episodes. Results Of 643 samples analysed, a virus was identified in 44%. Half were picornaviruses, with influenza and coronaviruses the next most common. Individuals with influenza were significantly less likely to have been immunised than the reference (virus negative) population (OR=0 center dot 52 (0 center dot 31, 0 center dot 87) P=0 center dot 01). The mean symptom score (95% CI) reported by individuals with influenza was significantly higher than in all other episodes [Influenza: 10 center dot 2 (9 center dot 4, 10 center dot 9); Other: 7 center dot 4 (7 center dot 2, 7 center dot 7); Difference (95% CI): 2 center dot 5 (1 center dot 5, 3 center dot 5); P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9825873ee9317532248e8d80902a5da4Test