التفاصيل البيبلوغرافية
| العنوان: |
Overexpression of the genesPDC1andADH1activates glycerol conversion to ethanol in the thermotolerant yeastOgataea(Hansenula)polymorpha |
| المؤلفون: |
Marta V. Semkiv, Justyna Ruchala, Kostyantyn V. Dmytruk, Andriy A. Sibirny, Iwona Kata |
| المصدر: |
Yeast. 33:471-478 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, biology, Bioconversion, 030106 microbiology, Bioengineering, Ethanol fermentation, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Yeast, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Genetics, Glycerol, biology.protein, Ethanol fuel, Fermentation, Ogataea polymorpha, Biotechnology, Alcohol dehydrogenase |
| الوصف: |
Conversion of byproduct from biodiesel production glycerol to high-value compounds is of great importance. Ethanol is considered a promising product of glycerol bioconversion. The methylotrophic thermotolerant yeast Ogataea (Hansenula) polymorpha is of great interest for this purpose as the glycerol byproduct contains methanol and heavy metals as contaminants, and this yeast utilizes methanol and is relatively resistant to heavy metals. Besides, O. polymorpha shows robust growth on glycerol and produces ethanol from various carbon sources. The thermotolerance of this yeast is an additional advantage, allowing increased fermentation temperature to 45–48 °C, leading to increased rate of the fermentation process and a fall in the cost of distillation. The wild-type strain of O. polymorpha produces insignificant amounts of ethanol from glycerol (0.8 g/l). Overexpression of PDC1 coding for pyruvate decarboxylase enhanced ethanol production up to 3.1 g/l, whereas simultaneous overexpression of PDC1 and ADH1 (coding for alcohol dehydrogenase) led to further increase in ethanol production from glycerol. Moreover, the increased temperature of fermentation up to 45 °C stimulated the production of ethanol from glycerol used as the only carbon source up to 5.0 g/l, which exceeds the data obtained by methylotrophic yeast strains reported so far. Copyright © 2016 John Wiley & Sons, Ltd. |
| تدمد: |
0749-503X |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::b58080bb5438823970332c7d1eafe248Test
https://doi.org/10.1002/yea.3175Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........b58080bb5438823970332c7d1eafe248 |
| قاعدة البيانات: |
OpenAIRE |
