Heterologous expression of anti-apoptotic human 14-3-3β/α enhances iron-mediated programmed cell death in yeast
| العنوان: | Heterologous expression of anti-apoptotic human 14-3-3β/α enhances iron-mediated programmed cell death in yeast |
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| المؤلفون: | Nagla T.T. Arab, Rawan Eid, Michael T. Greenwood, David R. Zhou, Craig A. Mandato, Eric Boucher, Paul G. Young |
| المصدر: | PLoS ONE, Vol 12, Iss 8, p e0184151 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Mutant, Gene Expression, lcsh:Medicine, Apoptosis, Yeast and Fungal Models, Vacuole, medicine.disease_cause, lcsh:Science, Cellular Stress Responses, Mutation, Multidisciplinary, Cell Death, Organic Compounds, Chemistry, Monosaccharides, Cell biology, Experimental Organism Systems, Cell Processes, Physical Sciences, Research Article, Programmed cell death, Iron, Autophagic Cell Death, Carbohydrates, Saccharomyces cerevisiae, Research and Analysis Methods, Necrotic Cell Death, Saccharomyces, 03 medical and health sciences, Model Organisms, otorhinolaryngologic diseases, medicine, Humans, Cell growth, Organic Chemistry, lcsh:R, Organisms, Fungi, Chemical Compounds, Biology and Life Sciences, Galactose, Cell Biology, Yeast, 030104 developmental biology, 14-3-3 Proteins, Ferritins, lcsh:Q, Heterologous expression, Copper |
| الوصف: | The induction of Programmed Cell Death (PCD) requires the activation of complex responses involving the interplay of a variety of different cellular proteins, pathways, and processes. Uncovering the mechanisms regulating PCD requires an understanding of the different processes that both positively and negatively regulate cell death. Here we have examined the response of normal as well as PCD resistant yeast cells to different PCD inducing stresses. As expected cells expressing the pro-survival human 14-3-3β/α sequence show increased resistance to numerous stresses including copper and rapamycin. In contrast, other stresses including iron were more lethal in PCD resistant 14-3-3β/α expressing cells. The increased sensitivity to PCD was not iron and 14-3-3β/α specific since it was also observed with other stresses (hydroxyurea and zinc) and other pro-survival sequences (human TC-1 and H-ferritin). Although microscopical examination revealed little differences in morphology with iron or copper stresses, cells undergoing PCD in response to high levels of prolonged copper treatment were reduced in size. This supports the interaction some forms of PCD have with the mechanisms regulating cell growth. Analysis of iron-mediated effects in yeast mutant strains lacking key regulators suggests that a functional vacuole is required to mediate the synergistic effects of iron and 14-3-3β/α on yeast PCD. Finally, mild sub-lethal levels of copper were found to attenuate the observed inhibitory effects of iron. Taken together, we propose a model in which a subset of stresses like iron induces a complex process that requires the cross-talk of two different PCD inducing pathways. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d534713df9c2e93039ed7c990b56e94Test https://doi.org/10.1371/journal.pone.0184151Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9d534713df9c2e93039ed7c990b56e94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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