Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance
| العنوان: | Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance |
|---|---|
| المؤلفون: | Martínez Miguel, Marc, Kyvik, Adriana R., Ernst, Lena M., Martínez Moreno, Albert, Cano-Garrido, Olivia, Garcia-Fruitos, Elena, Vázquez Gómez, Esther, Ventosa, Nora, Guasch, Judith, Veciana i Miró, Jaume, Villaverde Corrales, Antonio, Ratera Bastardas, Imma, Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia |
| المساهمون: | Dirección General de Investigación Científica y Técnica, DGICT (España), Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), European Cooperation in Science and Technology, Fundació La Marató de TV3, European Commission, Max Planck Society, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Instituto de Salud Carlos III, Institut National de la Recherche Agronomique (France) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Materials Chemistry B Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Inclusion-bodies, Adhesion dynamics, Surface Properties, Biomedical Engineering, Nanoparticle, 02 engineering and technology, 010402 general chemistry, medicine.disease_cause, Cell morphology, 01 natural sciences, Inclusion bodies, Protein nanoparticles, chemistry.chemical_compound, Tissue engineering, Bacterial Proteins, Lactic acid bacteria, medicine, Escherichia coli, Tumor Cells, Cultured, Molecule, Humans, General Materials Science, Clinical settings, Particle Size, Biochemical stimuli, Cellular environment, biology, Molecular Structure, Chemistry, Optical Imaging, Nanoscale topography, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, biology.organism_classification, 3. Good health, 0104 chemical sciences, Lactic acid, Lactococcus lactis, Artificial tissues, Gram-negative bacteria, Extracellular-matrix, Biophysics, Nanoparticles, 0210 nano-technology, Bacteria |
| الوصف: | In tissue engineering, biological, physical, and chemical inputs have to be combined to properly mimic cellular environments and successfully build artificial tissues which can be designed to fulfill different biomedical needs such as the shortage of organ donors or the development of in vitro disease models for drug testing. Inclusion body-like protein nanoparticles (pNPs) can simultaneously provide such physical and biochemical stimuli to cells when attached to surfaces. However, this attachment has only been made by physisorption. To provide a stable anchoring, a covalent binding of lactic acid bacteria (LAB) produced pNPs, which lack the innate pyrogenic impurities of Gram-negative bacteria like Escherichia coli, is presented. The reported micropatterns feature a robust nanoscale topography with an unprecedented mechanical stability. In addition, they are denser and more capable of influencing cell morphology and orientation. The increased stability and the absence of pyrogenic impurities represent a step forward towards the translation of this material to a clinical setting. The authors are grateful for the financial support received from MOTHER (MAT2016-80826-R) granted by the DGI (Spain), GenCat (SGR-918 and SGR-229) financed by DGR (Catalunya), the Spanish Ministry of Economy and Competitiveness (MINECO) through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV-2015-0496), the COST Action CA15126 Between Atom and Cell, Fundació La Marató de TV3 (Nr. 201812), the European Social Fund, and EU to J. V., A. V., and I. R. (H2020-INFRAIA-2014-2015; NFFA-654360). J. G. is also grateful to MINECO for a “Ramón y Cajal” fellowship as well as to the Max Planck Society through the Max Planck Partner Group “Dynamic Biomimetics for Cancer Immunotherapy” in collaboration with the Max Planck Institute for Medical Research (Heidelberg, Germany). This study has been also supported by the Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), an initiative funded by the VI National R&D&I Plan, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. The authors also acknowledge Micalis Institute, INRA, France that kindly provided us the strain clpP− htrA−-NZ9000 (patent Nr. EP1141337B1/US6994997B1). M. M. M. and O. C. G. received a PhD fellowship from MECD (FPU) and E. G. F. a post-doctoral fellowship from INIA (DOC-INIA, MINECO). A. V. received an ICREA ACADEMIA Award. This work has been developed under the biochemistry and biomedicine program of Universitat Autònoma de Barcelona (UAB). The characterization of the pNPs has been performed by the ICTS “NANBIOSIS”, more specifically by the Biomaterial Processing and Nanostructuring Unit (U6), Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) located at the Institute of Materials Science of Barcelona (ICMAB-CSIC). |
| وصف الملف: | application/pdf |
| تدمد: | 2050-7518 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::876961fdf452a5c6011e0f64268cadf9Test https://pubmed.ncbi.nlm.nih.gov/32400840Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....876961fdf452a5c6011e0f64268cadf9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20507518 |
|---|