Laser ablation ICP-MS for simultaneous quantitative imaging of iron and ferroportin in hippocampus of human brain tissues with Alzheimer's disease
| العنوان: | Laser ablation ICP-MS for simultaneous quantitative imaging of iron and ferroportin in hippocampus of human brain tissues with Alzheimer's disease |
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| المؤلفون: | Aurora Astudillo, Susana Junceda, María Cruz-Alonso, Beatriz Fernández, Rosario Pereiro, Ana Navarro |
| المصدر: | Scopus RUO. Repositorio Institucional de la Universidad de Oviedo instname RUO: Repositorio Institucional de la Universidad de Oviedo Universidad de Oviedo (UNIOVI) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Iron, Ferroportin, 02 engineering and technology, Mass spectrometry, 01 natural sciences, Hippocampus, Mass Spectrometry, Analytical Chemistry, chemistry.chemical_compound, Nuclear magnetic resonance, Alzheimer Disease, medicine, Humans, Inductively coupled plasma mass spectrometry, Cation Transport Proteins, Carbodiimide, Laser ablation, biology, Chemistry, 010401 analytical chemistry, Optical Imaging, Human brain, 021001 nanoscience & nanotechnology, Fluorescence, 0104 chemical sciences, medicine.anatomical_structure, Case-Control Studies, biology.protein, Laser Therapy, Inductively coupled plasma, 0210 nano-technology |
| الوصف: | Laser ablation inductively coupled plasma - mass spectrometry (LA-ICP-MS) is proposed for a better understanding of metals and proteins distribution in micrometre structures of human brain tissues. Simultaneous absolute quantitative imaging of Fe and ferroportin (FPN), in 5 µm thick tissue sections of the stratum pyramidale of hippocampus CA1 region, was carried out for Alzheimer disease (AD) patients and healthy controls (HC). For the imaging of FPN by LA-ICP-MS, antibodies were labelled via carbodiimide crosslinking with fluorescent gold nanoclusters (AuNCs) of 2.2 nm diameter, enabling a high amplification (314 gold atoms per NC). Laboratory made gelatin standards containing Fe and Au were used for LA-ICP-MS calibration. Results showed that iron presents an increased concentration in AD donors compared with HC donors, whereas similar concentrations of FPN in AD donors with respect to HC donors were obtained. The average absolute FPN concentrations in selected areas obtained with the proposed AuNCs method were compared with the levels obtained by densitometric analysis with a traditional IHC approach, observing a similar trend in all cases. |
| تدمد: | 1873-3573 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84b6600d0f31e8fa861c089fbc335bd4Test https://pubmed.ncbi.nlm.nih.gov/30771955Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....84b6600d0f31e8fa861c089fbc335bd4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733573 |
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