Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection
| العنوان: | Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection |
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| المؤلفون: | Xu Dong, Gangyi Shen, Danrong Huang, Ruifeng Hu, Shan Chang, Dongsheng Dai, Weifei Lan |
| المصدر: | Chemical Research in Chinese Universities. 38:529-534 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Detection limit, Nanocomposite, biology, Oxide, Substrate (chemistry), 02 engineering and technology, General Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Michaelis–Menten kinetics, 0104 chemical sciences, Catalysis, chemistry.chemical_compound, chemistry, biology.protein, 0210 nano-technology, Hydrogen peroxide, Peroxidase, Nuclear chemistry |
| الوصف: | In this study, palladium nanoparticles loaded graphdiyne oxide (Pd/GDYO) nanocomposite were fabricated by in-situ reduction of palladium chloride in the dispersion of GDYO, and characterized by Raman spectra, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The synthesized Pd/GDYO was first found to have catalytic activities similar to those of the peroxidase enzyme, which can catalyze the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine(TMB) in the presence of hydrogen peroxide(H2O2). Steady-state kinetic studies showed that the catalytic reaction of Pd/GDYO follows a ping-pong mechanism, and Pd/GDYO has a stronger activity to TMB with a Michaelis constant(Km) value of 5.32×10−4 mmol/L. Based on the shielding effect of glutathione(GSH) on the Pd/GDYO-H2O2-TMB reaction system, a colorimetric detection method for GSH was developed with a wide linear range from 0.1 µmol/L to 40 µmol/L and a limit of detection of 0.1 µmol/L. The method was successfully applied for fast and accurate detection of GSH in injection powder drugs. It was expected that this peroxidase-like Pd/GDYO nanocomposite would have wide applications in the fields of biomedicine and environmental chemistry. |
| تدمد: | 2210-3171 1005-9040 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::1d6efb5d719924d810be7c67381cf10cTest https://doi.org/10.1007/s40242-021-1038-1Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........1d6efb5d719924d810be7c67381cf10c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22103171 10059040 |
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