-
1
المصدر: PLoS ONE, Vol 16, Iss 7, p e0254076 (2021)
PLoS ONEمصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Chlorophyll, Pigments, Salinity, Chloroplasts, Agricultural Irrigation, Physiology, Fungal Physiology, Colony Count, Microbial, Plant Science, 01 natural sciences, Physical Chemistry, Salt Stress, law.invention, chemistry.chemical_compound, Electrolytes, law, Plant Products, Microbial Physiology, Mycorrhizae, Medicinal Plants, Mycorrhiza, Chlorophyll fluorescence, Materials, Principal Component Analysis, Multidisciplinary, biology, Essential Oils, Eukaryota, Agriculture, Mentha piperita, Phosphorus, Plants, Photochemical Processes, Droughts, Horticulture, Chemistry, Plant Physiology, Physical Sciences, Metabolome, Medicine, Piriformospora, Cellular Structures and Organelles, Cellular Types, Research Article, Plant Cell Biology, Science, Materials Science, Mycology, Microbiology, Plant use of endophytic fungi in defense, Fluorescence, 03 medical and health sciences, Plant Cells, Oils, Volatile, Seawater, Symbiosis, Essential oil, Analysis of Variance, Organic Pigments, Terpenes, Basidiomycota, Sodium, fungi, Organisms, Fungi, Biology and Life Sciences, Photosystem II Protein Complex, Cell Biology, biology.organism_classification, Menthone, Agronomy, Plant Leaves, Species Interactions, 030104 developmental biology, chemistry, Chemical Properties, Potassium, Quantum Theory, Caspian Sea, 010606 plant biology & botany, Crop Science
الوصف: Symbiotic associations with endophytic fungi are ecologically important for medicinal and aromatic plants. Endophytic fungi highly affect the quantity and quality of herbal products. In this study, a pot experiment was carried out in the greenhouse to investigate the interactive effects of Piriformospora indica and arbuscular mycorrhizal (AMF) inoculation on the chlorophyll fluorescence, essential oil composition, and antioxidant enzymes of peppermint under saline condition. The results showed that Fo, YNPQ, YNO, and NPQ values were obviously increased under salinity conditions, while essential oil content, chlorophyll a and b, gs, Fm, Fv, ETR, ФPSII and Fv/Fm ratio decreased by increasing salinity. In addition, salt induced the excess Na+ uptake, whereas the opposite trend was observed for P and K+. The synergistic association of P. indica and AMF caused a considerable increase in the antioxidant ability, essential oil content, Fv/Fm ratio, ФPSII, and amount of P and K+ uptake in salt-stressed plants. The main peppermint oil constituents, menthol, menthone, and 1,8-cineole increased considerably in inoculated plants. Besides, the applied endophytic fungi positively enhanced the ability of peppermint to alleviate the negative effect of the salinity stress.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6da99b68420af723487cc6f1678a78caTest
https://doaj.org/article/d66df93bb61043e7814c9f2a6c64ee28Test -
2
المصدر: PLoS ONE, Vol 16, Iss 3, p e0247882 (2021)
PLoS ONEمصطلحات موضوعية: Chlorophyll, Pigments, 0106 biological sciences, 0301 basic medicine, Leaves, Chloroplasts, Light, Physiology, Plant Science, Biochemistry, 01 natural sciences, Vegetables, Photosynthesis, Vines, Materials, Multidisciplinary, biology, Plant Biochemistry, Chemistry, Plant Anatomy, Physics, Electromagnetic Radiation, Eukaryota, Plants, Horticulture, Physiological Parameters, Physical Sciences, Darkness, Medicine, Cellular Structures and Organelles, Cellular Types, Cucumis, Research Article, Plant Cell Biology, Science, Preservation, Biological, Materials Science, 03 medical and health sciences, Plant Cells, Transplanting, Lighting, Cucumber, Organic Pigments, Body Weight, Organisms, Biology and Life Sciences, Cell Biology, biology.organism_classification, Light intensity, 030104 developmental biology, Seedlings, Seedling, Cucumis sativus, Intensity (heat transfer), Photosynthetic photon flux, 010606 plant biology & botany
الوصف: Continuous darkness decreases seedling quality during storage, whereas appropriate light quality and intensity can overcome these negative effects. In this study, we determined the light intensity, storage time (ST), and storage temperature suitable for cucumber (Cucumis sativa L.) seedlings. We stored cucumber seedlings under four different photosynthetic photon flux densities (PPFDs; 0, 15, 30, and 45 μmol·m-2·s-1) at 12°C, and examined how the morphological, physiological, and photosynthetic changes in seedlings during storage affected their ability to recover after transplanting. Our results indicated that at least 15 μmol·m-2·s-1 PPFD was needed for cucumber seedlings stored in the dark for 2 or 4 d, and at least 30 μmol·m-2·s-1 PPFD was needed when the ST was extended to 6 d. Overall, our results showed that cucumber seedlings require light-emitting diode (LED) illumination during storage to maintain their quality and recovery ability.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7635c03d0fcbe59a8ace8a0f0a03d3eTest
https://doaj.org/article/1a4979cff68541e5bb7b9eb63607b8d0Test -
3
المؤلفون: Llamas, Ernesto, Pulido, Pablo, Rodriguez-Concepcion, Manuel
المساهمون: Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Consejo Nacional de Ciencia y Tecnología (México)
المصدر: Recercat. Dipósit de la Recerca de Catalunya
instname
PLoS Genetics, Vol 13, Iss 9, p e1007022 (2017)
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Digital.CSIC. Repositorio Institucional del CSIC
PLoS Geneticsمصطلحات موضوعية: Pigments, 0106 biological sciences, 0301 basic medicine, Chlorophyll, Cancer Research, Chloroplasts, Arabidopsis thaliana, Arabidopsis, Gene Expression, Plant Science, Biochemistry, 01 natural sciences, Heat Shock Response, Heat Shock Transcription Factors, Gene Expression Regulation, Plant, Photosynthesis, Heat-Shock Proteins, Genetics (clinical), Cellular Stress Responses, Plant Proteins, Regulation of gene expression, Heat shock response, Eukaryota, Endopeptidase Clp, Proteases, Plants, Plants, Genetically Modified, Enzymes, Lincomycin, DNA-Binding Proteins, Experimental Organism Systems, Chloroplast DNA, Cell Processes, Physical Sciences, Protein folding, Cellular Structures and Organelles, Cellular Types, Genome, Plant, Research Article, Signal Transduction, lcsh:QH426-470, Plant Cell Biology, Arabidopsis Thaliana, Materials Science, Brassica, Biology, Research and Analysis Methods, 03 medical and health sciences, Model Organisms, Plant and Algal Models, Plant Cells, Heat shock protein, Genetics, Transcription factors, Gene Regulation, HSP70 Heat-Shock Proteins, Heat shock, Molecular Biology, Materials by Attribute, Ecology, Evolution, Behavior and Systematics, Organic Pigments, Arabidopsis Proteins, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Regulatory Proteins, lcsh:Genetics, 030104 developmental biology, Seedlings, Enzymology, Unfolded Protein Response, Unfolded protein response, Gene expression, Transcription Factors, Molecular Chaperones, 010606 plant biology & botany
الوصف: Disruption of protein homeostasis in chloroplasts impairs the correct functioning of essential metabolic pathways, including the methylerythritol 4-phosphate (MEP) pathway for the production of plastidial isoprenoids involved in photosynthesis and growth. We previously found that misfolded and aggregated forms of the first enzyme of the MEP pathway are degraded by the Clp protease with the involvement of Hsp70 and Hsp100/ClpC1 chaperones in Arabidopsis thaliana. By contrast, the combined unfolding and disaggregating actions of Hsp70 and Hsp100/ClpB3 chaperones allow solubilization and hence reactivation of the enzyme. The repair pathway is promoted when the levels of ClpB3 proteins increase upon reduction of Clp protease activity in mutants or wild-type plants treated with the chloroplast protein synthesis inhibitor lincomycin (LIN). Here we show that LIN treatment rapidly increases the levels of aggregated proteins in the chloroplast, unleashing a specific retrograde signaling pathway that up-regulates expression of ClpB3 and other nuclear genes encoding plastidial chaperones. As a consequence, folding capacity is increased to restore protein homeostasis. This sort of chloroplast unfolded protein response (cpUPR) mechanism appears to be mediated by the heat shock transcription factor HsfA2. Expression of HsfA2 and cpUPR-related target genes is independent of GUN1, a central integrator of retrograde signaling pathways. However, double mutants defective in both GUN1 and plastome gene expression (or Clp protease activity) are seedling lethal, confirming that the GUN1 protein is essential for protein homeostasis in chloroplasts.
This work was funded by grants from the Spanish Ministry of Economy and Competitiveness (BIO2015-71703-REDT and BIO2014-59092-P) and Generalitat de Catalunya (2014SGR-1434). We also acknowledge the financial support from the Severo Ochoa Programme for Centres of Excellence in R&D 2016-2019 (SEV‐2015‐0533) and the CERCA Programme of the Generalitat de Catalunya. EL was supported by the Mexican CoNaCyT (PhD fellowships 421688 and “beca complemento”).وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60def7af6265844ca1263e9862940c6eTest
http://hdl.handle.net/2072/417132Test -
4
المؤلفون: Anthony J. E. Berndt, Amr Badary, Francis J. Fields, Tressa N Smalley, Vanessa Heredia, Ryan Simkovsky, Stephen P. Mayfield, Ashley E. Sproles, Yasin Torres-Tiji, Bijie Ren
المساهمون: Webber, Andrew
المصدر: PLoS ONE, Vol 16, Iss 11 (2021)
PloS one, vol 16, iss 11
PLoS ONE
PLoS ONE, Vol 16, Iss 11, p e0257089 (2021)مصطلحات موضوعية: RNA viruses, 0106 biological sciences, Chloroplasts, Coronaviruses, Physiology, Chlamydomonas reinhardtii, Plant Science, Biochemistry, 01 natural sciences, law.invention, Binding Analysis, law, Immune Physiology, Cloning, Molecular, Receptor, Lung, Pathology and laboratory medicine, Chlamydomonas Reinhardtii, 0303 health sciences, Immune System Proteins, Multidisciplinary, biology, Chemistry, Eukaryota, Medical microbiology, Plants, Recombinant Proteins, Spike Glycoprotein, Cell biology, Chloroplast, Infectious Diseases, Experimental Organism Systems, Viruses, Spike Glycoprotein, Coronavirus, Recombinant DNA, Pneumonia & Influenza, Medicine, SARS CoV 2, Pathogens, Cellular Structures and Organelles, Cellular Types, Research Article, Biotechnology, SARS coronavirus, Algae, General Science & Technology, Plant Cell Biology, Science, Immunology, Research and Analysis Methods, Microbiology, Antibodies, Vaccine Related, 03 medical and health sciences, Model Organisms, Protein Domains, Antigen, Plant and Algal Models, Plant Cells, Biodefense, Humans, Protein Interaction Domains and Motifs, Receptor Binding Assays, Chemical Characterization, 030304 developmental biology, Medicine and health sciences, Biology and life sciences, Prevention, Chlamydomonas, Organisms, Viral pathogens, Proteins, Molecular, Cell Biology, Pneumonia, biology.organism_classification, Fusion protein, Microbial pathogens, Coronavirus, Emerging Infectious Diseases, Animal Studies, Green algae, Generic health relevance, 010606 plant biology & botany, Cloning
الوصف: Recombinant production of viral proteins can be used to produce vaccine antigens or reagents to identify antibodies in patient serum. Minimally, these proteins must be correctly folded and have appropriate post-translation modifications. Here we report the production of the SARS-CoV-2 spike protein Receptor Binding Domain (RBD) in the green algae Chlamydomonas. RBD fused to a fluorescent reporter protein accumulates as an intact protein when targeted for ER-Golgi retention or secreted from the cell, while a chloroplast localized version is truncated. The ER-retained RBD fusion protein was able to bind the human ACE2 receptor, the host target of SARS-CoV-2, and was specifically out-competed by mammalian cell-produced recombinant RBD, suggesting that the algae produced proteins are sufficiently post-translationally modified to act as authentic SARS-CoV-2 antigens. Because algae can be grown at large scale very inexpensively, this recombinant protein may be a low cost alternative to other expression platforms.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b67f4049d879093e07cfa69a7e251c8cTest
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601568/?tool=EBITest -
5
المؤلفون: Pulido, Pablo, Llamas, Ernesto, Llorente, Briardo, Ventura, Salvador, Wright, Louwrance P., Rodríguez Concepción, Manuel, Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina \\'Vicent Villar Palasí\\'
المساهمون: European Commission, Ministerio de Economía y Competitividad (España), Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo, Generalitat de Catalunya, Consejo Nacional de Ciencia y Tecnología (México)
المصدر: Recercat. Dipósit de la Recerca de Catalunya
instname
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
PLoS Genetics, Vol 12, Iss 1, p e1005824 (2016)
PLoS Genetics
Digital.CSIC. Repositorio Institucional del CSICمصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Protein Folding, Cancer Research, Chloroplasts, Mutant, Arabidopsis, Plant Science, Biochemistry, 01 natural sciences, Plastids, Enzyme Chemistry, Heat-Shock Proteins, Genetics (clinical), biology, ATP synthase, Endopeptidase Clp, Proteases, Plants, Enzymes, 3. Good health, Immunoblot Analysis, Protein folding, Cellular Structures and Organelles, Cellular Types, Metabolic Networks and Pathways, Research Article, lcsh:QH426-470, Plant Cell Biology, Arabidopsis Thaliana, Molecular Probe Techniques, Brassica, Research and Analysis Methods, Enzyme Regulation, 03 medical and health sciences, Model Organisms, Transferases, Plant and Algal Models, Plant Cells, Heat shock protein, Genetics, Molecular Biology Techniques, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Arabidopsis Proteins, Terpenes, Organisms, Biology and Life Sciences, Proteins, Cell Biology, biology.organism_classification, Chaperone Proteins, lcsh:Genetics, 030104 developmental biology, Chaperone (protein), Proteolysis, Enzymology, biology.protein, Molecular Chaperones, 010606 plant biology & botany
الوصف: The lifespan and activity of proteins depend on protein quality control systems formed by chaperones and proteases that ensure correct protein folding and prevent the formation of toxic aggregates. We previously found that the Arabidopsis thaliana J-protein J20 delivers inactive (misfolded) forms of the plastidial enzyme deoxyxylulose 5-phosphate synthase (DXS) to the Hsp70 chaperone for either proper folding or degradation. Here we show that the fate of Hsp70-bound DXS depends on pathways involving specific Hsp100 chaperones. Analysis of individual mutants for the four Hsp100 chaperones present in Arabidopsis chloroplasts showed increased levels of DXS proteins (but not transcripts) only in those defective in ClpC1 or ClpB3. However, the accumulated enzyme was active in the clpc1 mutant but inactive in clpb3 plants. Genetic evidence indicated that ClpC chaperones might be required for the unfolding of J20-delivered DXS protein coupled to degradation by the Clp protease. By contrast, biochemical and genetic approaches confirmed that Hsp70 and ClpB3 chaperones interact to collaborate in the refolding and activation of DXS. We conclude that specific J-proteins and Hsp100 chaperones act together with Hsp70 to recognize and deliver DXS to either reactivation (via ClpB3) or removal (via ClpC1) depending on the physiological status of the plastid.
This work was funded by grants from the European Commission FP7 (TiMet and CarotenActors), Ministerio de Economia y Competitividad (BIO2011-23680 and BIO2014-59092-P), Programa iberoamericano de ciencia y tecnología para el desarrollo. (Ibercarot), and Generalitat de Catalunya (2014SGR-1434 and XRB) to MRC, and PhD fellowships from the Mexican Consejo Nacional de Ciencia y Tecnologia (421688 and beca-complemento) to EL.وصف الملف: application/pdf; application/zip
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0173b1698a7af685b08b79d436325957Test
http://hdl.handle.net/2072/415937Test -
6
المؤلفون: Allah Ditta, Luqman Riaz, Abdur Rehim, Muhammad Amjad Bashir, Ghulam Yasin, Muhammad Shah Jahan, Shafeeq Ur Rahman, Umbreen Shahzad, Zhenjie Du, Adnan Noor Shah, Qi Xuebin
المصدر: PLoS ONE, Vol 16, Iss 7, p e0253798 (2021)
PLoS ONEمصطلحات موضوعية: Chlorophyll, Pigments, 0106 biological sciences, Leaves, Chloroplasts, Plant Science, 010501 environmental sciences, Toxicology, Pathology and Laboratory Medicine, Heavy Metals, Biochemistry, 01 natural sciences, Antioxidants, Soil, chemistry.chemical_compound, Nutrient, Hydroponics, Medicine and Health Sciences, Toxins, Soil Pollutants, Materials, Triticum, Cadmium, Multidisciplinary, Plant Anatomy, Eukaryota, food and beverages, Plants, Hydrogen-Ion Concentration, Chemistry, Wheat, Physical Sciences, Toxicity, Shoot, Medicine, Cellular Structures and Organelles, Cellular Types, Research Article, Chemical Elements, Plant Cell Biology, Science, Materials Science, Toxic Agents, chemistry.chemical_element, Animal science, Plant Cells, Grasses, Proline, 0105 earth and related environmental sciences, Organic Pigments, Organisms, Biology and Life Sciences, Cell Biology, Hydrogen Peroxide, Carotenoids, Bioavailability, chemistry, Seedlings, Osmoprotectant, 010606 plant biology & botany
الوصف: Anthropogenic activities such as mining, manufacturing, and application of fertilizers release substantial quantities of cadmium (Cd) into the environment. In the natural environment, varying pH may play an important role in the absorption and accumulation of Cd in plants, which can cause toxicity and increase the risk to humans. We conducted a hydroponic experiment to examine the impact of pH on cadmium (Cd) solubility and bioavailability in winter wheat (Triticum aestivum L.) under controlled environmental conditions. The results showed that Cd concentration was significantly reduced in wheat with an increase in pH from 5 to 7, while it was dramatically increased at pH ranging from 7 to 9. However, in both cases, a significant reduction in physiological traits was observed. The addition of Cd (20, 50, and 200 μmol L-1) at all pH levels caused a substantial decline in wheat growth, chlorophyll and carotenoids contents, nutrient availability, while elevated cell membrane damage was observed in terms of electrolytic leakage (EL), osmoprotectants, and antioxidants activity. In our findings, the negative effects of acidic pH (5) on wheat growth and development were more pronounced in the presence of Cd toxicities. For instance, Cd concentration with 20, 50, and 200 μmol L-1 at acidic pH (5) reduced shoot dry biomass by 45%, 53%, and 79%, total chlorophyll contents by 26%, 41%, 56% while increased CAT activity in shoot by 109%, 175%, and 221%, SOD activity in shoot by 122%, 135%, and 167%, POD activity in shoot by 137%, 250%, and 265%, MDA contents in shoot by 51%, 83%, and 150%, H2O2 contents in shoot by 175%, 219%, and 292%, EL in shoot by 108%, 165%, and 230%, proline contents in shoot by 235%, 280%, and 393%, respectively as compared to neutral pH without Cd toxicities. On the other hand, neutral pH with Cd toxicities alleviated the negative effects of Cd toxicity on wheat plants by limiting Cd uptake, reduced reactive oxygen species (ROS) formation, and increased nutrient availability. In conclusion, neutral pH minimized the adverse effects of Cd stress by minimizing its uptake and accumulation in wheat plants.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::658f5be84c12fb2f49ee66ff819cf24bTest
https://doaj.org/article/a7728e805ba041808c124ab7e5413fc9Test -
7
المؤلفون: Chunxia He, Jun Gao, Qirui Wang, Jinsong Zhang
المصدر: PLoS ONE, Vol 16, Iss 7, p e0243954 (2021)
PLoS ONEمصطلحات موضوعية: Chlorophyll, Pigments, 0106 biological sciences, Leaves, Chloroplasts, Light, Plant Science, Plant Roots, 01 natural sciences, Trees, Quercus, chemistry.chemical_compound, Biomass, Materials, Light Pulses, Multidisciplinary, biology, Physics, Electromagnetic Radiation, Plant Anatomy, Eukaryota, 04 agricultural and veterinary sciences, Plants, Wood, Nitrogen, Horticulture, Distilled water, Physical Sciences, Sunlight, Medicine, Solar Radiation, Quercus variabilis, Cellular Structures and Organelles, Cellular Types, Cork, Cycling, Research Article, Plant Cell Biology, Science, Materials Science, chemistry.chemical_element, Oaks, Plant Cells, Glutamine synthetase, Nitrogen cycle, Nitrogen Isotopes, Organic Pigments, Organisms, Biology and Life Sciences, Cell Biology, biology.organism_classification, Plant Leaves, chemistry, Seedlings, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Shading, 010606 plant biology & botany
الوصف: Light spectra of sunlight transmittance can generate an interactive effect with deposited nitrogen (N) on regenerated plants across varied shading conditions. Total N content in understory plants can be accounted for by both exogeneous and endogenous sources of derived N, but knowledge about the response of inner N cycling to interactive light and N input effects is unclear. We conducted a bioassay on Chinese cork oak (Quercus variabilis Blume) seedlings subjected to five-month N pulsing with 15NH4Cl (10.39 atom %) at 120 mg 15N plant-1 under the blue (48.5% blue, 33.7% green, and 17.8% red), red (14.6% blue, 71.7% red, 13.7% green), and green (17.4% blue, 26.2% red, 56.4% green) lighting-spectra. Half of the seedlings were fed twice a week using a 250 ppm N solution with micro-nutrients, while the other half just received distilled water. Two factors showed no interaction and neither affected growth and morphology. Compared to the red-light spectrum, that in blue light increased chlorophyll and soluble protein contents and glutamine synthetase (GS) activity, root N concentration, and N derived from the pulses. The green-light spectrum induced more biomass allocation to roots and a higher percentage of N derived from internal reserves compared to the red-light spectrum. The 15N pulses reduced the reliance on N remobilization from acorns but strengthened shoot biomass, chlorophyll content, GS activity, and N concentration. In conclusion, light spectrum imposed an independent force from external N pulse to modify the proportion of N derived from internal sources in total N content in juvenile Q. variabilis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e57da0e1ca0617b6ce3a0719c3aeec05Test
https://doaj.org/article/aabde8e5fced4e96a292c42869582597Test -
8
المؤلفون: Jia Tan, Siri Larsen, Raeann Goering, Irina Makarevitch, James Whelan
المصدر: PLoS ONE, Vol 16, Iss 7, p e0254437 (2021)
PLoS ONEمصطلحات موضوعية: Chlorophyll, Pigments, 0106 biological sciences, 0301 basic medicine, Germplasm, Leaves, Candidate gene, Chloroplasts, Inbred Strains, Plant Science, 01 natural sciences, Inbred strain, Materials, education.field_of_study, Multidisciplinary, biology, Plant Anatomy, Physics, Temperature, Eukaryota, Classical Mechanics, food and beverages, Agriculture, Plants, Horticulture, Experimental Organism Systems, Germination, Physical Sciences, Mechanical Stress, Medicine, Cellular Structures and Organelles, Cellular Types, Research Article, Plant Cell Biology, Science, Quantitative Trait Loci, Materials Science, Population, Crops, Quantitative trait locus, Research and Analysis Methods, Zea mays, 03 medical and health sciences, Model Organisms, Plant and Algal Models, Plant Cells, Genetics, Grasses, education, Organic Pigments, Organisms, Biology and Life Sciences, Cell Biology, biology.organism_classification, Genetic architecture, Maize, Thermal Stresses, 030104 developmental biology, Seedlings, Genetic Loci, Seedling, Animal Studies, Crop Science, Cereal Crops, 010606 plant biology & botany
الوصف: Maize is a cold sensitive crop that exhibits severe retardation of growth and development when exposed to cold spells during and right after germination, including the slowdown in development of new leaves and in formation of the photosynthetic apparatus. Improving cold tolerance in maize would allow early sowing to improve crop yield by prolonging a growing season and by decreasing the negative effects of summer drought, diseases, and pests. Two maize inbreds widely incorporated into American maize germplasm, B73 and Mo17, exhibit different levels of tolerance to low temperature exposure at seedling stage. In addition, thirty seven diverse inbred maize lines showed large variation for seedling response to low temperature exposure with lines with extremely low tolerance to seedling exposure to low temperatures falling into stiff stalk, non-stiff stalk, and tropical clades. We employed the maize intermated B73×Mo17 (IBM) recombinant inbred line population (IBM Syn4 RIL) to investigate the genetic architecture of cold stress tolerance at a young seedling stage and to identify quantitative trait loci (QTLs) controlling this variation. A panel of 97 recombinant inbred lines of IBM Syn4 were used to measure, and score based on several traits related to chlorophyll concentration, leaf color, and tissue damage. Our analysis resulted in detection of two QTLs with high additive impact, one on chromosome 1 (bin 1.02) and second on chromosome 5 (bin 5.05). Further investigation of the QTL regions using gene expression data provided a list of the candidate genes likely contributing to the variation in cold stress response. Among the genes located within QTL regions identified in this study and differentially expressed in response to low temperature exposure are the genes with putative functions related to auxin and gibberellin response, as well as general abiotic stress response, and genes coding for proteins with broad regulatory functions.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f935a78e34b29a96fb2aad20eb8a1c6Test
https://doaj.org/article/2c49367dc62a4b84a4412cde207880d5Test -
9
المؤلفون: Zita Berki, Ottó Veisz, Krisztina Balla, Ádám Horváth, Tibor Kiss, P. Bónis, Ildikó Karsai, Tamás Árendás, András Cseh
المصدر: PLoS ONE, Vol 16, Iss 5, p e0252070 (2021)
PLoS ONEمصطلحات موضوعية: Chlorophyll, Pigments, 0106 biological sciences, 0301 basic medicine, Leaves, Chloroplasts, Physiology, Winter wheat, Plant Science, Biochemistry, 01 natural sciences, chemistry.chemical_compound, Plant Resistance to Abiotic Stress, Medicine and Health Sciences, Cultivar, Photosynthesis, Materials, Triticum, Plant stem, Multidisciplinary, Ecology, Plant Biochemistry, Phenology, Physics, Plant Anatomy, Classical Mechanics, Eukaryota, food and beverages, Plants, Heat stress, Horticulture, Process Engineering, Plant Physiology, Physical Sciences, Wheat, Mechanical Stress, Engineering and Technology, Medicine, Anatomy, Cellular Structures and Organelles, Cellular Types, Research Article, Heat Treatment, Chlorophyll content, Plant Cell Biology, Science, Materials Science, Industrial Processes, Biology, 03 medical and health sciences, Plant Cells, Plant-Environment Interactions, Industrial Engineering, Plant Defenses, Grasses, Organic Pigments, Plant Ecology, Ecology and Environmental Sciences, Organisms, Biology and Life Sciences, Cell Biology, Plant Pathology, Thermal Stresses, 030104 developmental biology, Manufacturing Processes, chemistry, Ears, Head, Heat-Shock Response, 010606 plant biology & botany
الوصف: With a possible reference to heat priming and to characterize the extent and variation in the heat stress responses in wheat, the effects of single vs. repeated heat stresses were examined by measuring the changes in morphological and grain yield-related traits and photosynthetic parameters. To achieve these objectives, 51 winter wheat cultivars of various geographic origins were included in two independent experiments covering different phenological stages. In Experiment I, a single heat stress event was applied at stem elongation (SE) and booting (B), and the repeated heat stress was applied at both of these stages (SE+B). In Experiment II, the single heat stress was applied at stem elongation (SE) and full heading (CH), while the repeated heat stress was applied at both stages (SE+CH). While genotype was a more important factor for determining the morphological and yield-related traits, it was the treatment effect that mostly influenced the photosynthetic parameters, with the exception of the chlorophyll content. The heading stage was more sensitive to heat stress than the booting stage, which was primarily due to the larger decrease in the average seed number. The importance of biomass in contributing to grain yield intensified with the heat stress treatments. There was a large variation between the wheat cultivars not only in yielding abilities under control conditions but also in sensitivities to the various heat stresses, based on which 7 distinct groups with specific response profiles could be identified at a highly significant level. The 7 wheat groups were also characterized by their reaction patterns of different magnitudes and directions in their responses to single vs. repeated heat stresses, which depended on the phenological phases during the second cycle of heat stress. The possible association between these findings and heat priming is discussed.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d65e46b710a4f7d2147cc9a0fbd90c1dTest
https://doaj.org/article/fcd101ae6ca547129620eef89bc9626dTest -
10
المؤلفون: Shirzad Kamali, Ahmad Mehraban
المصدر: PLoS ONE
PLoS ONE, Vol 15, Iss 12, p e0243824 (2020)مصطلحات موضوعية: 0106 biological sciences, Chlorophyll, Pigments, Leaves, Chloroplasts, Agricultural Irrigation, Meteorological Concepts, Physiology, Evaporation, Plant Science, 01 natural sciences, Biochemistry, chemistry.chemical_compound, Electrolytes, Natural Resources, Plant Resistance to Abiotic Stress, Mycorrhizae, Amino Acids, Water content, Materials, Glomus, Plant Proteins, Multidisciplinary, biology, Vaporization, Ecology, Organic Compounds, Physics, Plant Anatomy, food and beverages, Eukaryota, 04 agricultural and veterinary sciences, Plants, Spores, Fungal, Condensed Matter Physics, Droughts, Chemistry, Plant Physiology, Physical Sciences, Seeds, Water Resources, Medicine, Seasons, Cellular Structures and Organelles, Cellular Types, Phase Transitions, Research Article, Inflorescences, Proline, Science, Plant Cell Biology, Materials Science, Quantitative Trait, Heritable, Stress, Physiological, Plant Cells, Plant-Environment Interactions, Plant Defenses, Grasses, Panicles, Water-use efficiency, Fertilizers, Microbial inoculant, Sorghum, Panicle, Analysis of Variance, Azotobacter, Organic Pigments, Plant Ecology, fungi, Ecology and Environmental Sciences, Organic Chemistry, Organisms, Chemical Compounds, Biology and Life Sciences, Proteins, Water, Cyclic Amino Acids, Cell Biology, Plant Pathology, biology.organism_classification, Plant Leaves, chemistry, Agronomy, Solubility, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, 010606 plant biology & botany
الوصف: The use of bio-fertilizers in agro-ecosystems is considered to have the potential to improve plant growth in extreme environments featuring water shortages. However, while arbuscular mycorrhizal fungi (AMF) and bacteria bio-fertilizers have been used in other plants to enhance stress tolerance, little is known about their symbiotic effect on sorghum (Sorghum bicolor L.) growth under drought stress conditions. Therefore the aim of this study was to investigate the inoculation of sorghum with Nitroxin and Glomus mosseae and their interaction effects on the agro-physiological characteristics and grain yield of sorghum under drought stress conditions. Nitroxin is a bio-fertilizer that consists of a mixture of Azospirillum and Azotobacter bacteria. The results showed that co-inoculation of sorghum seeds with Nitroxin and AMF improved the chlorophyll (a, b and total) content, soluble proteins, water use efficiency) WUE(, relative water content (RWC), nitrogen (N) content in the plant, AMF spore density, proline content, grain yield, panicle length, the number of panicles per plant, grain number per panicle, 1000-grain weight and decreased the electrolyte leakage and water saturation deficit (WSD) in drought stress and non-stress conditions. Under drought stress conditions, there was a 27% increase in grain yield under the synergistic effects of bacteria and fungi compared to the non-application of these microorganisms. The results of this experiment show that Nitroxin and AMF bio-fertilizers can mitigate the negative effects of stress on plants in drought stress conditions by increasing the amount of photosynthetic pigments, soluble proteins and osmotic regulation and decreasing electrolyte leakage. We found that the combination of bacteria and AMF for sorghum growth and yield increment is a promising method to cope with the stress caused by drought.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32ebe72bee4d82f98e38c5519538aa92Test
http://europepmc.org/articles/PMC7769463Test
