المؤلفون: Jacob L. Gorenflos López, Tatyana Grayfer, Antri Georgiou, Chien-Chi Hsiao, Simon Sieber, Karl Gademann, Leo Eberl, Aurélien Bailly

المساهمون: University of Zurich, Eberl, Leo, Bailly, Aurélien

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Scientific Reports

مصطلحات موضوعية: 10120 Department of Chemistry, 0106 biological sciences, Arabidopsis, 580 Plants (Botany), 01 natural sciences, Pheromones, 10126 Department of Plant and Microbial Biology, Plant symbiosis, Phylogeny, 2. Zero hunger, 0303 health sciences, Multidisciplinary, biology, Cell wall, Plant morphogenesis, Lettuce, Cell biology, Seeds, Medicine, Root apical meristem, Allelopathy, Mustard Plant, Burkholderia, Science, Meristem, Germination, Plant cell biology, Article, 03 medical and health sciences, Plant development, Psychotria, 10211 Zurich-Basel Plant Science Center, Symbiosis, 030304 developmental biology, 1000 Multidisciplinary, Obligate, Plant Extracts, Cyclohexanols, biology.organism_classification, Plant cell, Plant Leaves, Cell wall organization, Seedlings, Function (biology), 010606 plant biology & botany

الوصف: After a century of investigations, the function of the obligate betaproteobacterial endosymbionts accommodated in leaf nodules of tropical Rubiaceae remained enigmatic. We report that the α-d-glucose analogue (+)-streptol, systemically supplied by mature Ca.Burkholderia kirkii nodules to their Psychotria hosts, exhibits potent and selective root growth inhibiting activity. We provide compelling evidence that (+)-streptol specifically affects meristematic root cells transitioning to anisotropic elongation by disrupting cell wall organization in a mechanism of action that is distinct from canonical cellulose biosynthesis inhibitors. We observed no inhibitory or cytotoxic effects on organisms other than seed plants, further suggesting (+)-streptol as a bona fide allelochemical. We propose that the suppression of growth of plant competitors is a major driver of the formation and maintenance of the Psychotria–Burkholderia association. In addition to potential agricultural applications as a herbicidal agent, (+)-streptol might also prove useful to dissect plant cell and organ growth processes.

وصف الملف: s41598-021-01867-2.pdf - application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c43d82b37c8c32043e69917164643876Test
https://doaj.org/article/fa73c84b271f49648365643bfb033395Test

المؤلفون: Shingo Nagawa, Lihong Wang, Zhenbiao Yang, Xiaohua Huang, Qing Zhou, Hui Li, Daiyin Chao, Mengzhu Cheng, Li Tan, Zhenhong Gu, Jiazhi Zhang, Ding He

المصدر: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Nature Communications

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Cell signaling, Science, Rare earth, Green Fluorescent Proteins, Arabidopsis, General Physics and Astronomy, chemistry.chemical_element, Plant cell biology, Cyclopentanes, Endocytosis, 01 natural sciences, Plant Roots, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Lanthanum, Plant Cells, Oxylipins, chemistry.chemical_classification, Reactive oxygen species, Minerals, Multidisciplinary, Growth promoting, biology, Arabidopsis Proteins, Jasmonic acid, fungi, NADPH Oxidases, food and beverages, General Chemistry, biology.organism_classification, Plants, Genetically Modified, Cell biology, Plant Leaves, 030104 developmental biology, chemistry, 010606 plant biology & botany, Cell signalling, Signal Transduction

الوصف: Trivalent rare earth elements (REEs) are widely used in agriculture. Aerially applied REEs enter leaf epidermal cells by endocytosis and act systemically to improve the growth of the whole plant. The mechanistic basis of their systemic activity is unclear. Here, we show that treatment of Arabidopsis leaves with trivalent lanthanum [La(III)], a representative of REEs, triggers systemic endocytosis from leaves to roots. La(III)-induced systemic endocytosis requires AtrbohD-mediated reactive oxygen species production and jasmonic acid. Systemic endocytosis impacts the accumulation of mineral elements and the development of roots consistent with the growth promoting effects induced by aerially applied REEs. These findings provide insights into the mechanistic basis of REE activity in plants.
Rare earth elements are widely used in agriculture to promote plant growth. Here the authors show that aerial application of a rare earth element to the leaves triggers a graft-transmissible, RbohD- and jasmonic acid-dependent systemic signal that triggers endocytosis and mineral nutrient uptake in roots.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48663230c105a1527109e05856737f6eTest
https://doaj.org/article/963581461b2945c296ea48eeb78e8b12Test

المؤلفون: Satoru Fujimoto, Hiroshi Kimura, Takuya Sakamoto, Mayu Yoshikawa, Satoshi Uchino, Tamako Yamaoka, Shusuke Kasamatsu, Yuko Sato, Sachihiro Matsunaga, Takamasa Suzuki, Mio Shibuta, Noriyoshi Yagi

المصدر: Communications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
Communications Biology

مصطلحات موضوعية: 0106 biological sciences, Fluorescence-lifetime imaging microscopy, QH301-705.5, Cell, Arabidopsis, Medicine (miscellaneous), RNA polymerase II, Plant cell biology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Article, Fluorescence imaging, 03 medical and health sciences, Spatio-Temporal Analysis, Transcription (biology), Live cell imaging, medicine, Arabidopsis thaliana, Phosphorylation, Biology (General), Mitosis, 030304 developmental biology, 0303 health sciences, biology, fungi, food and beverages, biology.organism_classification, Cell biology, Molecular Imaging, medicine.anatomical_structure, biology.protein, RNA Polymerase II, General Agricultural and Biological Sciences, Protein Processing, Post-Translational, 010606 plant biology & botany

الوصف: Spatiotemporal changes in general transcription levels play a vital role in the dynamic regulation of various critical activities. Phosphorylation levels at Ser2 in heptad repeats within the C-terminal domain of RNA polymerase II, representing the elongation form, is an indicator of transcription. However, rapid transcriptional changes during tissue development and cellular phenomena are difficult to capture in living organisms. We introduced a genetically encoded system termed modification-specific intracellular antibody (mintbody) into Arabidopsis thaliana. We developed a protein processing- and 2A peptide-mediated two-component system for real-time quantitative measurement of endogenous modification level. This system enables quantitative tracking of the spatiotemporal dynamics of transcription. Using this method, we observed that the transcription level varies among tissues in the root and changes dynamically during the mitotic phase. The approach is effective for achieving live visualization of the transcription level in a single cell and facilitates an improved understanding of spatiotemporal transcription dynamics.
Shibuta et al develop a live imaging system that allows them to track the spatiotemporal dynamics of RNA polymerase II modification in single cells in Arabidopsis thaliana. This approach potentially enables a more detailed understanding of transcriptional dynamics in plants.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54517ecacdd6aee25473723ebc57c702Test
https://doaj.org/article/14ed3675f57349db90dd2bb3b8b9edd3Test

المؤلفون: Kiminori Toyooka, Yoko Ito, Takashi Ueda, Mayuko Sato, Emi Ito, Junpei Takagi, Akihiko Nakano, Tomohiro Uemura, Yamato Komatsu, Kazuo Kurokawa, Yutaro Shimizu, Yumi Goto, Kazuo Ebine

المصدر: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature Communications

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Science, Arabidopsis, General Physics and Astronomy, Plant cell biology, macromolecular substances, 01 natural sciences, Clathrin, environment and public health, General Biochemistry, Genetics and Molecular Biology, Article, law.invention, R-SNARE Proteins, 03 medical and health sciences, symbols.namesake, Microscopy, Electron, Transmission, Live cell imaging, Confocal microscopy, law, Golgi, Super-resolution microscopy, Multidisciplinary, Microscopy, Confocal, biology, Arabidopsis Proteins, Signal transducing adaptor protein, General Chemistry, Golgi apparatus, biology.organism_classification, Plants, Genetically Modified, Transport protein, Cell biology, Luminescent Proteins, Protein Transport, 030104 developmental biology, Vacuoles, biology.protein, symbols, biological phenomena, cell phenomena, and immunity, 010606 plant biology & botany, trans-Golgi Network

الوصف: The trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different destinies still remains elusive. Here, we examined 3D localization and 4D dynamics of TGN-localized proteins of Arabidopsis thaliana that are involved in either secretory or vacuolar trafficking from the TGN, by a multicolor high-speed and high-resolution spinning-disk confocal microscopy approach that we developed. We demonstrate that TGN-localized proteins exhibit spatially and temporally distinct distribution. VAMP721 (R-SNARE), AP (adaptor protein complex)−1, and clathrin which are involved in secretory trafficking compose an exclusive subregion, whereas VAMP727 (R-SNARE) and AP-4 involved in vacuolar trafficking compose another subregion on the same TGN. Based on these findings, we propose that the single TGN has at least two subregions, or “zones”, responsible for distinct cargo sorting: the secretory-trafficking zone and the vacuolar-trafficking zone.
The trans-Golgi network (TGN) serves as a platform to sort and transport proteins to their final destinations. Here the authors show that the TGN of Arabidopsis consists of spatially and temporally distinct subregions and propose that these zones may sort cargo to different destinations.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a65134dfb2822656fc9964af1051767dTest
https://doaj.org/article/6244cab227394f20aa4c417a7cec0acdTest

المؤلفون: Brad Day, Sheng Yang He, Pai Li, Masaki Shimono, Takumi Higaki, Yi-Ju Lu, Alex Corrion

المصدر: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Nature Communications

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Cytoskeleton organization, animal diseases, Science, Arabidopsis, Pseudomonas syringae, General Physics and Astronomy, Plant cell biology, chemical and pharmacologic phenomena, macromolecular substances, 01 natural sciences, Article, General Biochemistry, Genetics and Molecular Biology, Actin cytoskeleton organization, 03 medical and health sciences, Plant immunity, Immunity, Amino Acid Sequence, Actin, Plant Diseases, Microscopy, Confocal, Multidisciplinary, Innate immune system, Sequence Homology, Amino Acid, biology, Arabidopsis Proteins, Effector, General Chemistry, biochemical phenomena, metabolism, and nutrition, Plants, Genetically Modified, biology.organism_classification, Cell biology, Actin Cytoskeleton, Effectors in plant pathology, 030104 developmental biology, Actin Depolymerizing Factors, Calcium-Calmodulin-Dependent Protein Kinases, Mutation, Phosphorylation, bacteria, 010606 plant biology & botany

الوصف: Pattern-triggered immunity and effector-triggered immunity are two primary forms of innate immunity in land plants. The molecular components and connecting nodes of pattern-triggered immunity and effector-triggered immunity are not fully understood. Here, we report that the Arabidopsis calcium-dependent protein kinase CPK3 is a key regulator of both pattern-triggered immunity and effector-triggered immunity. In vitro and in vivo phosphorylation assays, coupled with genetic and cell biology-based analyses, show that actin-depolymerization factor 4 (ADF4) is a physiological substrate of CPK3, and that phosphorylation of ADF4 by CPK3 governs actin cytoskeletal organization associated with pattern-triggered immunity. CPK3 regulates stomatal closure induced by flg22 and is required for resistance to Pst DC3000. Our data further demonstrates that CPK3 is required for resistance to Pst DC3000 carrying the effector AvrPphB. These results suggest that CPK3 is a missing link between cytoskeleton organization, pattern-triggered immunity and effector-triggered immunity.
Remodelling of the actin cytoskeleton occurs during plant immune responses to pathogens. Here Lu et al. show that this process requires the calcium-dependent kinase CPK3 which phosphorylates actin depolymerizing factor 4 and is required for both PAMP and effector-triggered immunity in Arabidopsis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::808808a001e791e6da412937750011c3Test
https://doaj.org/article/21196fb1f4894aee94540cebb52bf0ccTest

المؤلفون: Carsten Hille, Corné Swart, Youjun Zhang, Alexander Graf, Lee J. Sweetlove, Alisdair R. Fernie, Sandra Mae-Lin Kerber, Arun Sampathkumar, Kumar Seerangan

المصدر: Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
Nature Communications, 11, S. 4509
Nature Communications

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Chloroplasts, Plant molecular biology, Science, Pyruvate Kinase, Enolase, Arabidopsis, General Physics and Astronomy, Plant cell biology, Context (language use), Mitochondrion, 01 natural sciences, Article, General Biochemistry, Genetics and Molecular Biology, Phosphoglycerate mutase, 03 medical and health sciences, chemistry.chemical_compound, Biosynthesis, ddc:570, Protein Interaction Mapping, Glycolysis, Protein Interaction Maps, Photosynthesis, lcsh:Science, Phosphoglycerate Mutase, Multidisciplinary, Arabidopsis Proteins, food and beverages, General Chemistry, Plants, Genetically Modified, Mitochondria, 030104 developmental biology, Biochemistry, chemistry, Phosphopyruvate Hydratase, Mutation, lcsh:Q, Metabolon, Energy Metabolism, Pyruvate kinase, 010606 plant biology & botany

الوصف: Glycolysis is one of the primordial pathways of metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine the wider protein-protein interactions of plant glycolytic enzymes and reveal a moonlighting role for specific glycolytic enzymes in mediating the co-localization of mitochondria and chloroplasts. Knockout mutation of phosphoglycerate mutase or enolase resulted in a significantly reduced association of the two organelles. We provide evidence that phosphoglycerate mutase and enolase form a substrate-channelling metabolon which is part of a larger complex of proteins including pyruvate kinase. These results alongside a range of genetic complementation experiments are discussed in the context of our current understanding of chloroplast-mitochondrial interactions within photosynthetic eukaryotes.
Protein-protein interactions are thought to channel substrates between consecutive enzymes during glycolysis. Here the authors show that Arabidopsis phosphoglycerate mutase and enolase can form a substrate-channelling metabolon and also play a moonlighting role in promoting colocalization of chloroplasts and mitochondria.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6723c7b00c0e144148cb414514dd2af9Test
https://doaj.org/article/137e330c2bad4291b755d37b97898257Test

المؤلفون: Nico De Storme, Danny Geelen

المصدر: Communications Biology, Vol 3, Iss 1, Pp 1-15 (2020)
Communications Biology
COMMUNICATIONS BIOLOGY

مصطلحات موضوعية: Life Sciences & Biomedicine - Other Topics, 0106 biological sciences, 0301 basic medicine, DNA End-Joining Repair, Hot Temperature, Arabidopsis, RECOMBINATION, Medicine (miscellaneous), Interference (genetic), 01 natural sciences, Chromosomal crossover, Cell Wall, Gene Expression Regulation, Plant, Crossing Over, Genetic, lcsh:QH301-705.5, Synapsis, MEIOSIS, Plants, Genetically Modified, Cell biology, Multidisciplinary Sciences, Meiosis, Synaptonemal complex, Science & Technology - Other Topics, Pollen, General Agricultural and Biological Sciences, Life Sciences & Biomedicine, HEAT-STRESS, Karyotype, POLYPLOIDIZATION, Plant cell biology, ORGANIZATION, Biology, Chromosomes, Plant, Article, General Biochemistry, Genetics and Molecular Biology, MECHANISMS, Evolution, Molecular, Plant reproduction, 03 medical and health sciences, SYNAPTONEMAL COMPLEX, Sister chromatids, VISUAL ASSAY, Telophase, Science & Technology, Biology and Life Sciences, biology.organism_classification, CHROMOSOME SYNAPSIS, 030104 developmental biology, lcsh:Biology (General), Plant stress responses, CELLS, Heat-Shock Response, 010606 plant biology & botany

الوصف: Plant fertility is highly sensitive to elevated temperature. Here, we report that hot spells induce the formation of dyads and triads by disrupting the biogenesis or stability of the radial microtubule arrays (RMAs) at telophase II. Heat-induced meiotic restitution in Arabidopsis is predominantly SDR-type (Second Division Restitution) indicating specific interference with RMAs formed between separated sister chromatids. In addition, elevated temperatures caused distinct deviations in cross-over formation in male meiosis. Synapsis at pachytene was impaired and the obligate cross-over per chromosome was discarded, resulting in partial univalency in meiosis I (MI). At diakinesis, interconnections between non-homologous chromosomes tied separate bivalents together, suggesting heat induces ectopic events of non-homologous recombination. Summarized, heat interferes with male meiotic cross-over designation and cell wall formation, providing a mechanistic basis for plant karyotype change and genome evolution under high temperature conditions.
de Storme and Geelen show that heat stress has pleiotropic effects on male meiosis in Arabidopsis, causing deviations in cross-over formations, reproduction, and fertility. They show that heat also affects cell wall formation, providing mechanistic insights into karyotype change under high temperatures.

وصف الملف: Electronic; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93ccf637d19d60306255da59c05bb694Test
http://link.springer.com/article/10.1038/s42003-020-0897-1Test

المؤلفون: Xu Wang, Kristine Hill, Malcolm J. Bennett, Byung Chun Yoo, Ross Sager, Thu M. Tran, Jeffery L. Caplan, Alex Nedo, Jung-Youn Lee

المصدر: Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Science, Regulator, Arabidopsis, General Physics and Astronomy, Plant cell biology, Plasmodesma, 01 natural sciences, Plant Roots, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Auxin, Gene Expression Regulation, Plant, Negative feedback, Plant development, Plant hormones, Arabidopsis thaliana, Primordium, heterocyclic compounds, lcsh:Science, chemistry.chemical_classification, Regulation of gene expression, Multidisciplinary, biology, Indoleacetic Acids, Arabidopsis Proteins, Lateral root, fungi, Plasmodesmata, Membrane Proteins, food and beverages, General Chemistry, biology.organism_classification, Cell biology, 030104 developmental biology, chemistry, Plant signalling, lipids (amino acids, peptides, and proteins), lcsh:Q, Plant Shoots, 010606 plant biology & botany, Signal Transduction

الوصف: Lateral roots originate from initial cells deep within the main root and must emerge through several overlying layers. Lateral root emergence requires the outgrowth of the new primordium (LRP) to coincide with the timely separation of overlying root cells, a developmental program coordinated by the hormone auxin. Here, we report that in Arabidopsis thaliana roots, auxin controls the spatiotemporal expression of the plasmodesmal regulator PDLP5 in cells overlying LRP, creating a negative feedback loop. PDLP5, which functions to restrict the cell-to-cell movement of signals via plasmodesmata, is induced by auxin in cells overlying LRP in a progressive manner. PDLP5 localizes to plasmodesmata in these cells and negatively impacts organ emergence as well as overall root branching. We present a model, incorporating the spatiotemporal expression of PDLP5 in LRP-overlying cells into known auxin-regulated LRP-overlying cell separation pathways, and speculate how PDLP5 may function to negatively regulate the lateral root emergence process.
Auxin promotes lateral root emergence from pericycle cells in the root vasculature. Here the authors show that induction of the plasmodesmal regulator PDLP5 during lateral root emergence restricts the spatial scope of auxin signaling to the cells overlying the primordia.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6031c788c1a07b9df44ea4319c9a57f6Test
http://link.springer.com/article/10.1038/s41467-019-14226-7Test

المؤلفون: Wei Zhang, Lin Shi, Ying Jing, Jianwei Gao, Lilong He, Xin Li, Han Zheng, Mei Wang

المصدر: Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Salinity, Plant molecular biology, Plant physiology, Mutant, Arabidopsis, lcsh:Medicine, Plant cell biology, 01 natural sciences, Article, Transcriptome, 03 medical and health sciences, Gene Expression Regulation, Plant, Stress, Physiological, lcsh:Science, Gene, Regulation of gene expression, Multidisciplinary, biology, Arabidopsis Proteins, Chemistry, Gene Expression Profiling, lcsh:R, High-Throughput Nucleotide Sequencing, Reproducibility of Results, Salt Tolerance, biology.organism_classification, Cell biology, DNA-Binding Proteins, Gene expression profiling, Phenotype, 030104 developmental biology, Cytoplasm, Plant stress responses, Gene chip analysis, lcsh:Q, Transcription Factors, 010606 plant biology & botany

الوصف: Salt stress is one of the abiotic stresses affecting crop growth and yield. The functional screening and mechanism investigation of the genes in response to salt stress are essential for the development of salt-tolerant crops. Here, we found that OXIDATIVE STRESS 2 (OXS2) was a salinity-induced gene, and the mutant oxs2-1 was hypersensitive to salt stress during seed germination and root elongation processes. In the absence of stress, OXS2 was predominantly localized in the cytoplasm; when the plants were treated with salt, OXS2 entered the nuclear. Further RNA-seq analysis and qPCR identification showed that, in the presence of salt stress, a large number of differentially expressed genes (DEGs) were activated, which contain BOXS2 motifs previously identified as the binding element for AtOXS2. Further ChIP analysis revealed that, under salt stress, OXS2 associated with CA1 and Araport11 directly through binding the BOXS2 containing fragments in the promoter regions. In conclusion, our results indicate that OXS2 is required for salt tolerance in Arabidopsis mainly through associating with the downstream CA1 and Araport11 directly.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3649695d59ab5603aa7bf3c454180e2Test
http://link.springer.com/article/10.1038/s41598-019-56456-1Test

المؤلفون: Kalyan Mahapatra, Sujit Roy

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-26 (2021)

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Programmed cell death, Cell cycle checkpoint, Plant molecular biology, DNA, Plant, DNA damage, Science, Arabidopsis, Plant cell biology, Cell Cycle Proteins, Ataxia Telangiectasia Mutated Proteins, Cyclin B, Protein Serine-Threonine Kinases, Sodium Chloride, 01 natural sciences, Salt Stress, Article, Polyploidy, 03 medical and health sciences, Gene Expression Regulation, Plant, Plant Cells, Plant development, Endoreduplication, Transcription factor, Cell Size, Multidisciplinary, biology, Arabidopsis Proteins, Salt Tolerance, Cell cycle, biology.organism_classification, Cyclin-Dependent Kinases, Cell biology, E2F Transcription Factors, G2 Phase Cell Cycle Checkpoints, Wee1, 030104 developmental biology, biology.protein, Medicine, 010606 plant biology & botany, Signal Transduction, Transcription Factors

الوصف: As like in mammalian system, the DNA damage responsive cell cycle checkpoint functions play crucial role for maintenance of genome stability in plants through repairing of damages in DNA and induction of programmed cell death or endoreduplication by extensive regulation of progression of cell cycle. ATM and ATR (ATAXIA-TELANGIECTASIA-MUTATED and -RAD3-RELATED) function as sensor kinases and play key role in the transmission of DNA damage signals to the downstream components of cell cycle regulatory network. The plant-specific NAC domain family transcription factor SOG1 (SUPPRESSOR OF GAMMA RESPONSE 1) plays crucial role in transducing signals from both ATM and ATR in presence of double strand breaks (DSBs) in the genome and found to play crucial role in the regulation of key genes involved in cell cycle progression, DNA damage repair, endoreduplication and programmed cell death. Here we report that Arabidopsis exposed to high salinity shows generation of oxidative stress induced DSBs along with the concomitant induction of endoreduplication, displaying increased cell size and DNA ploidy level without any change in chromosome number. These responses were significantly prominent in SOG1 overexpression line than wild-type Arabidopsis, while sog1 mutant lines showed much compromised induction of endoreduplication under salinity stress. We have found that both ATM-SOG1 and ATR-SOG1 pathways are involved in the salinity mediated induction of endoreduplication. SOG1was found to promote G2-M phase arrest in Arabidopsis under salinity stress by downregulating the expression of the key cell cycle regulators, including CDKB1;1, CDKB2;1, and CYCB1;1, while upregulating the expression of WEE1 kinase, CCS52A and E2Fa, which act as important regulators for induction of endoreduplication. Our results suggest that Arabidopsis undergoes endoreduplicative cycle in response to salinity induced DSBs, showcasing an adaptive response in plants under salinity stress.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27dcfa00b075eacc19ea923a10ac1036Test
http://europepmc.org/articles/PMC8172935Test