Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments
| العنوان: | Altered germination and subcellular localization patterns for PUB44/SAUL1 in response to stress and phytohormone treatments |
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| المؤلفون: | Jennifer N. Salt, Wolfgang Moeder, Keiko Yoshioka, Daphne R. Goring |
| المصدر: | PLoS ONE, Vol 6, Iss 6, p e21321 (2011) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | 0106 biological sciences, Programmed cell death, Ubiquitin-Protein Ligases, Arabidopsis Thaliana, Plant Cell Biology, Mutant, Arabidopsis, lcsh:Medicine, Germination, Plant Science, Plant Genetics, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Model Organisms, Plant Growth Regulators, Gene Expression Regulation, Plant, Plant and Algal Models, Arabidopsis thaliana, lcsh:Science, Biology, Abscisic acid, 030304 developmental biology, Plant Growth and Development, 0303 health sciences, Multidisciplinary, biology, Arabidopsis Proteins, lcsh:R, Plants, Genetically Modified, biology.organism_classification, Subcellular localization, chemistry, Biochemistry, Cytoplasm, Seedling, lcsh:Q, Abscisic Acid, Research Article, 010606 plant biology & botany |
| الوصف: | Background In plants, the ubiquitin-proteasome system is emerging as a significant regulatory system throughout the plant lifecycle. The ubiquitination of a target protein requires the sequential actions of the E1, E2 and E3 enzymes, with the latter E3 enzyme conferring target selection in this process. There are a large number of predicted E3 enzymes in plant genomes, and very little is known about the functions of many of these predicted genes. Here we report here an analysis of two closely-related members of the Arabidopsis Plant U-box (PUB) family of E3 ubiquitin ligases, PUB43 and PUB44. Principal Findings Homozygous pub44/pub44 mutant seedlings were found displayed a seedling lethal phenotype and this corresponded with widespread cell death lesions throughout the cotyledons and roots. Interestingly, heterozygous PUB44/pub44 seedlings were wild-type in appearance yet displayed intermediate levels of cell death lesions in comparison to pub44/pub44 seedlings. In contrast, homozygous pub43/pub43 mutants were viable and did not show any signs of cell death despite the PUB43 gene being more highly expressed than PUB44. The PUB44 mutants are not classical lesion mimic mutants as they did not have increased resistance to plant pathogens. We also observed increased germination rates in mutant seeds for both PUB44 and PUB43 under inhibitory concentrations of abscisic acid. Finally, the subcellular localization of PUB44 was investigated with transient expression assays in BY-2 cells. Under varying conditions, PUB44 was observed to be localized to the cytoplasm, plasma membrane, or nucleus. Conclusions Based on mutant plant analyses, the Arabidopsis PUB43 and PUB44 genes are proposed to function during seed germination and early seedling growth. Given PUB44's ability to shuttle from the nucleus to the plasma membrane, PUB44 may be active in different subcellular compartments as part of these biological functions. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc940e7b922c072a63104e8f4eb4d14Test http://europepmc.org/articles/PMC3124495?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebc940e7b922c072a63104e8f4eb4d14 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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