المؤلفون: Hyun Kyung Lee, Daphne R. Goring

المصدر: Journal of experimental botany. 72(4)

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Gynoecium, Physiology, Mutant, Arabidopsis, Plant Science, Flowers, Pollen Tube, Biology, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Pollen, otorhinolaryngologic diseases, medicine, Ovule, Arabidopsis Proteins, fungi, food and beverages, Sperm, Pollen hydration, Sexual reproduction, Cell biology, 030104 developmental biology, Pollen tube, Protein Kinases, 010606 plant biology & botany

الوصف: In flowering plants, cell–cell communication between the compatible pollen grain/growing pollen tube and the pistil is an essential component for successful sexual reproduction. In Arabidopsis thaliana, the later stages of this dialogue are mediated by several peptide ligands and receptors that guide pollen tubes to the ovules for the release of sperm cells. Despite a detailed understanding of these processes, a key gap remains regarding the nature of the regulators that function at the earlier stages which are essential steps leading to fertilization. Here, we report on new functions for A. thaliana Receptor-Like Kinase (RLK) genes belonging to the LRR-II and LRR-VIII-2 RLK subgroups in the female reproductive tract to regulate compatible pollen hydration and the early stages of pollen tube growth. Mutant pistils for the A. thaliana RKF1 gene cluster were observed to support reduced wild-type pollen hydration and, when combined with the SERK1 and SERK3/BAK1 mutations, reduced pollen tube travel distances occurred. As these mutant pistils displayed a wild-type morphology, we propose that the observed altered compatible pollen responses result from an impaired pollen–pistil dialogue at these early stages.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dfc4ec3606f11f247e474df6ef20cd4Test
https://pubmed.ncbi.nlm.nih.gov/33097927Test

المؤلفون: Daphne R. Goring, Hyun Kyung Lee

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, Gynoecium, food and beverages, Biology, medicine.disease_cause, biology.organism_classification, 01 natural sciences, Pollen hydration, Cell biology, Sexual reproduction, 03 medical and health sciences, Pollen, Arabidopsis, medicine, otorhinolaryngologic diseases, Arabidopsis thaliana, Pollen tube, Ovule, 030304 developmental biology, 010606 plant biology & botany

الوصف: SummaryIn flowering plants, continuous cell-cell communication between the compatible male pollen grain/growing pollen tube and the female pistil is required for successful sexual reproduction. In Arabidopsis thaliana, the later stages of this dialogue are mediated by several peptide ligands and receptor kinases that guide pollen tubes to the ovules for the release of sperm cells. Despite a detailed understanding of these processes, a key gap remains on the nature of the regulators that function at the earlier stages. Here, we report on two groups of A. thaliana receptor kinases, the LRR-VIII-2 RK subclass and the SERKs, that function in the female reproductive tract to regulate the compatible pollen grains and early pollen tube growth, both essential steps for the downstream processes leading to fertilization. Multiple A. thaliana LRR-VIII-2 RK and SERK knockout mutant combinations were created, and several phenotypes were observed such as reduced wild-type pollen hydration and reduced pollen tube travel distances. As these mutant pistils displayed a wild-type morphology, the observed altered responses of the wild-type pollen are proposed to result from the loss of these receptor kinases leading to an impaired pollen-pistil dialogue at these early stages. Furthermore, using pollen from related Brassicaceae species, we also discovered that these receptor kinases are required in the female reproductive tract to establish a reproductive barrier to interspecies pollen. Thus, we propose that the LRR-VIII-2 RKs and the SERKs play a dual role in the preferential selection and promotion of intraspecies pollen over interspecies pollen.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d96343009d4787b9b7c6bd867e005caTest

المؤلفون: Moritz K. Nowack, Christina Truong, Anna Daneva, Hyun Kyung Lee, Elizabeth Frank-Webb, Zhen Gao, Jennifer Doucet, Daphne R. Goring

مصطلحات موضوعية: 0106 biological sciences, Gynoecium, Mutant, Arabidopsis, Germination, Plant Science, Flowers, Pollen Tube, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Gene Expression Regulation, Plant, Pollen, medicine, Pollen adhesion, Pollination, Gene, Phylogeny, 030304 developmental biology, Genetics, 0303 health sciences, biology, Arabidopsis Proteins, Reproduction, food and beverages, Cell Biology, biology.organism_classification, Pollen hydration, Organ Specificity, Seedlings, Mutation, Intercellular Signaling Peptides and Proteins, Pollen tube, Transcriptome, 010606 plant biology & botany

الوصف: In Arabidopsis, successful pollen-stigma interactions are dependent on rapid recognition of compatible pollen by the stigmatic papillae located on the surface of the pistil and the subsequent regulation of pollen hydration and germination, and followed by the growth of pollen tubes through the stigma surface. Here we have described the function of a novel gene, E6-like 1 (E6L1), that was identified through the analysis of transcriptome datasets, as one of highest-expressed genes in the stigma, and furthermore, its expression was largely restricted to the stigma and trichomes. The first E6 gene was initially identified as a highly-expressed gene during cotton fiber development, and related E6-like predicted proteins are found throughout the Angiosperms. To date, no orthologous genes have been assigned a biological function. Both the Arabidopsis E6L1 and cotton E6 proteins are predicted to be secreted, and this was confirmed using an E6L1:RFP fusion construct. To further investigate E6L1’s function, one T-DNA and two independent CRISPR-generated mutants were analyzed for compatible pollen-stigma interactions, and pollen hydration, pollen adhesion and seed set were mildly impaired for the e6l1 mutants. This work identifies E6L1 as a novel stigmatic factor that plays a role during the early post-pollination stages in Arabidopsis.Key MessageWe describe a function for a novel Arabidopsis gene, E6-like 1 (E6L1), that was identified as a highly-expressed gene in the stigma and plays a role in early post-pollination stages.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91448bca86e1a1e5f3bae67e65981355Test
https://doi.org/10.1101/623686Test

المؤلفون: Daphne R. Goring

المصدر: Journal of experimental botany. 69(1)

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Gynoecium, Pollination, Physiology, Arabidopsis, Vesicular Transport Proteins, Exocyst, Plant Science, Brassica, Pollen Tube, Biology, medicine.disease_cause, Exosomes, 01 natural sciences, 03 medical and health sciences, Pollen, Botany, otorhinolaryngologic diseases, medicine, Autophagy, Secretion, Secretory Pathway, food and beverages, biology.organism_classification, Pollen hydration, Cell biology, Protein Transport, 030104 developmental biology, Pollen tube, 010606 plant biology & botany

الوصف: Brassicaceae pollen-stigma interactions have been extensively studied in Brassica and Arabidopsis species to identify cellular events triggered in the stigmatic papillae by pollen contact. Compatible pollinations are linked to the activation of basal cellular responses in the stigmatic papillae, which include calcium gradients, actin networks, and polarized secretion. The occurrence of these cellular events in stigmatic papillae coincides with the stages of pollen hydration and pollen tube entry into the stigmatic papillar cell wall. However, the form of the vesicle trafficking appears to differ between species, with vesicle-like structures detected in Arabidopsis species while exosomes were found to be secreted in Brassica species. Around the same timeframe, self-incompatible pollen recognition leads altered cellular responses in the stigmatic papillae to interfere with basal compatible pollen responses and disrupt regulated secretion, causing self-pollen rejection. Here, the literature on the changing cellular dynamics in the stigmatic papillae following pollination is reviewed and discussed in the context of other well-characterized examples of polarized secretion in plants.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40ddde18b58afb5024a13e4de6e10339Test
https://pubmed.ncbi.nlm.nih.gov/29036428Test

المؤلفون: Darya Safavian, Daphne R. Goring, Emily Indriolo, Abdalla Ahmed, Laura A. Chapman, Yara Zayed

المصدر: Plant Physiology. :00635.2015

مصطلحات موضوعية: 0106 biological sciences, Pollination, Physiology, Protein subunit, Arabidopsis, Exocyst, Flowers, Pollen Tube, Plant Science, Biology, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Pollen, Botany, otorhinolaryngologic diseases, Genetics, medicine, 030304 developmental biology, 0303 health sciences, Arabidopsis Proteins, Cell Membrane, food and beverages, Articles, biology.organism_classification, Pollen hydration, Cell biology, RNA silencing, RNA Interference, Pollen tube, 010606 plant biology & botany

الوصف: Initial pollen-pistil interactions in the Brassicaceae are regulated by rapid communication between pollen grains and stigmatic papillae and are fundamentally important, as they are the first step toward successful fertilization. The goal of this study was to examine the requirement of exocyst subunits, which function in docking secretory vesicles to sites of polarized secretion, in the context of pollen-pistil interactions. One of the exocyst subunit genes, EXO70A1, was previously identified as an essential factor in the stigma for the acceptance of compatible pollen in Arabidopsis (Arabidopsis thaliana) and Brassica napus. We hypothesized that EXO70A1, along with other exocyst subunits, functions in the Brassicaceae dry stigma to deliver cargo-bearing secretory vesicles to the stigmatic papillar plasma membrane, under the pollen attachment site, for pollen hydration and pollen tube entry. Here, we investigated the functions of exocyst complex genes encoding the remaining seven subunits, SECRETORY3 (SEC3), SEC5, SEC6, SEC8, SEC10, SEC15, and EXO84, in Arabidopsis stigmas following compatible pollinations. Stigma-specific RNA-silencing constructs were used to suppress the expression of each exocyst subunit individually. The early postpollination stages of pollen grain adhesion, pollen hydration, pollen tube penetration, seed set, and overall fertility were analyzed in the transgenic lines to evaluate the requirement of each exocyst subunit. Our findings provide comprehensive evidence that all eight exocyst subunits are necessary in the stigma for the acceptance of compatible pollen. Thus, this work implicates a fully functional exocyst complex as a component of the compatible pollen response pathway to promote pollen acceptance.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17264d9062948ae19d653fa0bad30451Test
https://doi.org/10.1104/pp.15.00635Test