المؤلفون: Antonio Brante, Pascal Riera, Cédric Leroux, Thierry Comtet, Fanny Leroy

المساهمون: Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), DIVersité et COnnectivité dans le paysage marin côtier (DIVCO), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB), Ministere de la Recherche et de l'Enseignement Superieur, INSU, IFREMER [2009/10371755]

المصدر: Journal Of Molluscan Studies (0260-1230) (Oxford Univ Press), 2012-02, Vol. 78, N. 1, P. 100-104
Journal of Molluscan Studies
Journal of Molluscan Studies, Oxford University Press (OUP), 2012, 78 (1), pp.100-104. ⟨10.1093/mollus/eyr044⟩
Journal of Molluscan Studies, 2012, 78 (1), pp.100-104. ⟨10.1093/mollus/eyr044⟩

مصطلحات موضوعية: 0106 biological sciences, chemistry.chemical_classification, animal structures, food.ingredient, [SDV]Life Sciences [q-bio], 010604 marine biology & hydrobiology, Embryogenesis, Capsule, Embryo, Aquatic Science, Biology, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Organic molecules, Amino acid, food, Biochemistry, chemistry, Yolk, [SDE]Environmental Sciences, embryonic structures, Dissolved organic carbon, Animal Science and Zoology, Crepidula

الوصف: International audience; Many marine gastropod species brood their embryos in thin-walled capsules to protect them during development. Despite its beneficial effects, encapsulation has two major constraints, nutrition and oxygen supply, which affect embryo development and larval survival. Developing embryos usually rely on intracapsular food sources provided by the mother, in the form of yolk, nurse eggs and intracapsular fluid. However, it is still not clear if they are able to feed on extracapsular sources that may cross the capsule wall. We investigated this possibility in the calyptraeid species Crepidula fornicata. In this species, the internal capsule wall thickness sharply decreases during embryonic development, which might change wall permeability to small organic molecules, thus providing embryos with external dissolved organic matter. To test this hypothesis, encapsulated and excapsulated embryos of C. fornicata were placed for 48 h in a C-13-enriched amino acid (L-alanine) solution. Excapsulated embryos were enriched in C-13 (+5.75%), which suggested that they were able to assimilate the labelled amino acid. In contrast, encapsulated embryos were weakly enriched (+0.75%), suggesting that encapsulation greatly reduces the potential for the use of extracapsular amino acids and that encapsulated embryos mainly rely on maternal food.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5da25cdf425fcab9a800dcbc13170aabTest
https://doi.org/10.1093/mollus/eyr044Test

المؤلفون: Fanny Leroy, Pascal Riera, Thierry Comtet, Tarik Meziane

المساهمون: Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), DIVersité et COnnectivité dans le paysage marin côtier (DIVCO), Ministere de la Recherche et de l'Enseignement Superieur, INSU [2009/10371755]

المصدر: PLoS ONE
PLoS ONE, 2013, 8 (9), pp.e75316. ⟨10.1371/journal.pone.0075316⟩
PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e75316. ⟨10.1371/journal.pone.0075316⟩
PLoS ONE, Vol 8, Iss 9, p e75316 (2013)
Plos One (1932-6203) (Public Library Science), 2013-09, Vol. 8, N. 9, P. e75316 (1-19)

مصطلحات موضوعية: 0106 biological sciences, Embryo, Nonmammalian, animal structures, Offspring, Acclimatization, Gastropoda, lcsh:Medicine, Zoology, Biology, 010603 evolutionary biology, 01 natural sciences, Animals, Juvenile, 14. Life underwater, Crepidula, Maternal Behavior, Muscle, Skeletal, lcsh:Science, chemistry.chemical_classification, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, Larva, Multidisciplinary, Ecology, 010604 marine biology & hydrobiology, Fatty Acids, lcsh:R, Fatty acid, Maternal Nutritional Physiological Phenomena, Marine invertebrates, biology.organism_classification, Viscera, chemistry, Female, lcsh:Q, France, Seasons, Research Article, Polyunsaturated fatty acid

الوصف: International audience; Recruitment success of marine invertebrate populations not only depends on the number of recruits but also on their quality which affects their survival. In species characterized by a mixed development (encapsulated embryonic development and release of planktotrophic larvae), the offspring quality depends on both maternal provisioning and larval feeding. Here, we investigated potential changes of maternal provisioning over the whole reproductive period in a gastropod with a mixed development: Crepidula fornicata. In its introduction area, C. fornicata reproduces from February to October, which implies that both adults and larvae are exposed to different food availabilities. Maternal provisioning was assessed by measuring the fatty acid (FA) composition of females, encapsulated embryos and larvae, in February, May, July and September 2009. FA are essential resources for the development of embryos and larvae, and are key biomarkers of offspring quality. Our results showed differences in FA composition between muscles, visceral masses, and encapsulated embryos. In particular, FA composition of embryos was similar to that of the visceral mass. Seasonal variations in FA composition were observed: in the middle of the reproductive season (May and July), female tissues and embryos showed a higher proportion of polyunsaturated fatty acids and especially.3, as compared to the beginning and end of the reproductive season (February and September). This showed that through maternal provisioning the quality of C. fornicata offspring was higher in the middle of the reproductive season. Whether this would result in an increase of recruitment success and juvenile performance would require further investigations.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ec9a0eeb7774c422552a41104038d3dTest
https://hal.science/hal-01218825/documentTest

المؤلفون: Christian Jeanthon, Pascal Riera, Frédérique Edmond, Cédric Leroux, Thierry Comtet, Fanny Leroy

المساهمون: Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecogéochimie et Fonctionnement des Ecosystèmes Benthiques (EFEB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Procaryotes Phototrophes Marins = MArine Phototrophic Prokaryotes (MAPP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), DIVersité et COnnectivité dans le paysage marin côtier (DIVCO), Ministere de la Recherche et de l'Enseignement superieur, INSU [2009/10371755], INTERREG, MArine Phototrophic Prokaryotes (MAPP)

المصدر: Journal of Sea Research (JSR)
Journal of Sea Research (JSR), 2012, 70, pp.23-31. ⟨10.1016/j.seares.2012.02.006⟩
Journal of Sea Research (JSR), Elsevier, 2012, 70, pp.23-31. ⟨10.1016/j.seares.2012.02.006⟩

مصطلحات موضوعية: 0106 biological sciences, [SDV]Life Sciences [q-bio], Aquatic Science, Oceanography, 010603 evolutionary biology, 01 natural sciences, Botany, Phytoplankton, 14. Life underwater, Crepidula, Crepidula fornicata, Ecology, Evolution, Behavior and Systematics, Invertebrate, Diatoms, Larva, biology, Bacteria, Stable isotope ratio, 010604 marine biology & hydrobiology, fungi, Larval Feeding, Pelagic zone, biology.organism_classification, Diatom, C-13 and N-15, [SDE]Environmental Sciences, Stable Isotope Labeling

الوصف: International audience; In Europe, the gastropod Crepidula fornicata is an invasive species characterized by a long reproductive period (from February to November). Thus, its larvae are exposed to variations in available food sources (in terms of quantity and quality). We aimed to investigate if bacteria could contribute to larval food both in presence or absence of phytoplankton, and to compare these results to seasonal variations of bacteria and phytoplankton abundances at a coastal site in the English Channel. First, ingestion of fluorescent beads of 0.5 to 2 um diameter, showed that larvae were able to ingest particles of typical bacterial size. Then we used a dual stable isotope labeling approach which consisted in labeling a bacterial pelagic community with N-15 and a diatom (Chaetoceros gracilis) culture with C-13, and supplying larvae with N-15-labeled bacteria, C-13-labeled diatoms, and both labeled sources. This technique has, to our knowledge, never been applied to invertebrate larvae. After 24 h of experiment, larvae were significantly enriched in all treatments: +21.5 parts per thousand (Delta delta C-13) when supplied with diatoms, +1364 parts per thousand (Delta delta N-15) when supplied with bacteria, and +24 parts per thousand (Delta delta C-13) and +135 parts per thousand (Delta delta N-15) when supplied with the two mixed sources. These results indicated that bacteria can contribute to the larval nutrition in C. fornicata, even in the presence of phytoplankton. Our results however suggested that larvae of C. fornicata preferentially used diatoms and showed that the supply of free bacteria did not alter the uptake of diatoms. Considering the seasonal variations of bacteria and phytoplankton abundances at the study site, these results suggested that bacteria may constitute a complementary resource for the larvae of C. fornicata when phytoplankton is abundant and may become a substitute resource when phytoplankton is less available. This approach offers promising perspectives to trace food sources and assess nitrogen and carbon fluxes between planktotrophic larvae and their preys. (C) 2012 Elsevier B.V. All rights reserved.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04d1f0b9e9c25bd4e23e48f40cff9784Test
https://hal.science/hal-01218827Test