The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology
| العنوان: | The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species—a potential new tool in entomology |
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| المؤلفون: | Joscelyn Sarsby, Tom A. R. Price, Jane L. Hurst, Robert J. Beynon, Natalie I Koch, Sam Jones, Nicola White, Iris Wagner |
| المصدر: | Open Biology, Vol 10, Iss 11 (2020) Open Biology |
| بيانات النشر: | The Royal Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Data Analysis, Male, Entomology, Immunology, species identification, Biology, Mass spectrometry, 010603 evolutionary biology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Biological specimen, Species Specificity, Melanogaster, Animals, Sample preparation, Ionization mass spectrometry, insects, Drosophila, lcsh:QH301-705.5, 030304 developmental biology, mass spectrometry, 0303 health sciences, General Neuroscience, Research, drosophila, biology.organism_classification, lcsh:Biology (General), Evolutionary biology, Mass spectrum, reims, Female, Research Article |
| الوصف: | There is increasing emphasis on the use of new analytical approaches in subject analysis and classification, particularly in respect to minimal sample preparation. Here, we demonstrate that rapid evaporative ionization mass spectrometry (REIMS), a method that captures metabolite mass spectra after rapid combustive degradation of an intact biological specimen, generates informative mass spectra from several arthropods, and more specifically, is capable of discerning differences between species and sex of several adult Drosophila species. A model including five Drosophila species, built using pattern recognition, achieves high correct classification rates (over 90%) using test datasets and is able to resolve closely related species. The ease of discrimination of male and female specimens also demonstrates that sex-specific differences reside in the REIMS metabolite patterns, whether analysed across all five species or specifically for D. melanogaster. Further, the same approach can correctly discriminate and assign Drosophila species at the larval stage, where these are morphologically highly similar or identical. REIMS offers a novel approach to insect typing and analysis, requiring a few seconds of data acquisition per sample and has considerable potential as a new tool for the field biologist. |
| اللغة: | English |
| تدمد: | 2046-2441 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb082bdb2d40d7496df1d3c7e29a2573Test https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200196Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bb082bdb2d40d7496df1d3c7e29a2573 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20462441 |
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