Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism
| العنوان: | Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism |
|---|---|
| المؤلفون: | Alan Ling Yang, Vanessa Guo, Hsi Chien Huang, Steven Ting-Yu Chou, Yen Ju Chen, Anthony Yan-Tang Wu, Charles P. Lai, Chantal Hoi Yin Cheung, Hsueh Fen Juan, Syuan Wu, Meng-Ru Ho, Koji Ueda, John J. Ko, Yunching Chen, Ju Chen Chuang, Maria Ericsson, Yun Chieh Sung, Jasper Che-Yung Chien, Wan-Wan Lin |
| المصدر: | Advanced Science, Vol 7, Iss 19, Pp n/a-n/a (2020) Advanced Science |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Biodistribution, General Chemical Engineering, General Physics and Astronomy, Medicine (miscellaneous), exosomes, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), In vivo, redirected tropism, Extracellular, Bioluminescence, General Materials Science, lcsh:Science, biodistribution, Tropism, Full Paper, General Engineering, Full Papers, 021001 nanoscience & nanotechnology, Microvesicles, 0104 chemical sciences, Membrane protein, bioluminescence resonance energy transfer, Biophysics, exomeres, lcsh:Q, extracellular vesicles, 0210 nano-technology, microvesicles, Intracellular |
| الوصف: | Extracellular particles (EPs) including extracellular vesicles (EVs) and exomeres play significant roles in diseases and therapeutic applications. However, their spatiotemporal dynamics in vivo have remained largely unresolved in detail due to the lack of a suitable method. Therefore, a bioluminescence resonance energy transfer (BRET)‐based reporter, PalmGRET, is created to enable pan‐EP labeling ranging from exomeres (200 nm) EVs. PalmGRET emits robust, sustained signals and allows the visualization, tracking, and quantification of the EPs from whole animal to nanoscopic resolutions under different imaging modalities, including bioluminescence, BRET, and fluorescence. Using PalmGRET, it is shown that EPs released by lung metastatic hepatocellular carcinoma (HCC) exhibit lung tropism with varying distributions to other major organs in immunocompetent mice. It is further demonstrated that gene knockdown of lung‐tropic membrane proteins, solute carrier organic anion transporter family member 2A1, alanine aminopeptidase/Cd13, and chloride intracellular channel 1 decreases HCC‐EP distribution to the lungs and yields distinct biodistribution profiles. It is anticipated that EP‐specific imaging, quantitative assays, and detailed in vivo characterization are a starting point for more accurate and comprehensive in vivo models of EP biology and therapeutic design. PalmGRET, a bioluminescence resonance energy transfer (BRET)‐based reporter for extracellular particles (EPs), enables pan‐EP labeling, including extracellular vesicles and exomeres. PalmGRET allows accurate visualization, tracking, and quantification of EPs from whole animal to nanoscopic resolutions under different imaging modalities, including bioluminescence, BRET, and fluorescence. Using PalmGRET, lung‐tropic EP proteins are identified and dynamically altered biodistributions are revealed under redirected tropism. |
| تدمد: | 2198-3844 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ecbd26ea23805ac1557b063ae46aeccTest https://doi.org/10.1002/advs.202001467Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7ecbd26ea23805ac1557b063ae46aecc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21983844 |
|---|