A Palette of Minimally Tagged Sucrose Analogues for Real‐Time Raman Imaging of Intracellular Plant Metabolism
| العنوان: | A Palette of Minimally Tagged Sucrose Analogues for Real‐Time Raman Imaging of Intracellular Plant Metabolism |
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| المؤلفون: | Ailsa Geddis, Karl Oparka, Marc Vendrell, Anke Reinders, Martin Lee, Kirsten Knox, William J. Tipping, Fabio de Moliner, Doireann Gordon, John M. Ward |
| المصدر: | De Moliner, F, Knox, K, Gordon, D, Lee, M, Tipping, W J, Geddis, A, Reinders, A, Ward, J, Oparka, K & Vendrell, M 2021, ' A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism ', Angewandte Chemie International Edition . https://doi.org/10.1002/anie.202016802Test Angewandte Chemie (International Ed. in English) Moliner, F, Knox, K, Gordon, D, Lee, M, Tipping, W J, Geddis, A, Reinders, A, Ward, J M, Oparka, K & Vendrell, M 2021, ' A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism ', Angewandte Chemie International Edition . https://doi.org/10.1002/anie.202016802Test Angewandte Chemie International Edition |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Azides, Sucrose, Cell Membrane Permeability, Imaging Agents, Spectrum Analysis, Raman, 010402 general chemistry, 01 natural sciences, Catalysis, chemistry.chemical_compound, symbols.namesake, Coumarins, Plant Cells, Yeasts, Moiety, QD, Plant Proteins, Microscopy, Chemistry, 010405 organic chemistry, Communication, imaging, Membrane Transport Proteins, Fructose, Transporter, General Chemistry, General Medicine, Plant cell, Communications, 0104 chemical sciences, Kinetics, Biochemistry, sugars, Alkynes, transport, symbols, Metabolome, stimulated Raman scattering, Indicators and Reagents, Raman spectroscopy, probes, Intracellular, Raman scattering |
| الوصف: | Sucrose is the main saccharide used for long‐distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real‐time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne‐tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real‐time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy. We describe a chemical strategy to prepare sucrose derivatives with vibrational tags and the characterisation of the first optical analogue of sucrose able to image real‐time trafficking of sucrose in live plant cells. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1433-7851 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c902c68932dcc4031e0537b71c4677cTest https://hdl.handle.net/20.500.11820/11f99062-c25b-4da7-9f66-ba8afcb2ad27Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4c902c68932dcc4031e0537b71c4677c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14337851 |
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