التفاصيل البيبلوغرافية
| العنوان: |
基于液相色谱 - 串联质谱技术（LC-MS/MS）检测癫痫小鼠毛发中内源性 大麻素（2-AG、AEA）的含量. |
| العنوان البديل: |
Liquid Chromatography and Tandem Mass Spectrometry Determined the Content (2-AG, AEA) Endocenic Cannabinoids in Epileptic Mice. |
| المؤلفون: |
马富鑫1, 任 盼1, 阮 哲2, 陈建梅3, 王 莉3 wlsx127@163.com |
| المصدر: |
Progress in Modern Biomedicine. Mart2023, Vol. 23 Issue 6, p1006-1009. 4p. |
| مصطلحات موضوعية: |
*LIQUID chromatography-mass spectrometry, *SOLID phase extraction, *TANDEM mass spectrometry, *CANNABINOIDS, *EPILEPSY |
| مصطلحات جغرافية: |
TRIPOLI (Libya) |
| الملخص (بالإنجليزية): |
Objective: The content of endocannabinoids（2-AG, AEA) was examined based on liquid chromatography and tandem mass spectrometry (LC-MS/MS） in epileptic mice. Methods: The mouse epilepsy model was established by pilocarpine peritoneal injection, methanol incubation and solid phase extraction column extraction, qualitatively tested under atmospheric chemical ionizing positive ion mode and multi-reaction monitoring, quantitatively tested 2-AG and AEA content by liquid chromatography and series mass spectrometry, compared the differences in 2-AG and AEA content of different seizure grades of epileptic mice. Result: The retention times of AEA and 2-AG in the standard solution were 14.3 min, 18.1 min, and those of AEA and 2-AG in the hair samples were 14.0min, 17.7 min, respectively. Hair AEA, 2-AG detection limits were 0.7 pg/mg and 3.2 pg/mg, quantitative limits of 2.1 pg/mg and 10.9pg/mg, respectively, the recovery rates were 97.9% and 99.3%, respectively, and the day and daytime coefficient of variation was less than 15%. AEA and 2-AG were increased in grade seizures, grade 1, grade 3, grade 4, and grade 2, and AEA and 2-AG were compared in hair samples of different seizure grades（P＜0.05）. Conclusion: The determination of endocannabinoids（2-AG, AEA） used by LC-MS/MS has the advantages of higher sensitivity and less sample use, and is conducive to large-scale sample research. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：基于液相色谱 - 串联质谱技术 (LC-MS/MS) 对癫痫小鼠毛发中内源性大麻素 (2-AG、AEA) 进行含量检测。方法：匹鲁 卡品腹腔注射方法建立小鼠癫痫模型，采用 LC-MS/MS 检测毛发中 2-AG、AEA 的含量，对比分析不同癫痫发作分级小鼠 2-AG、AEA 含量差异。结果：标准品溶液中 AEA、2-AG 的保留时间分别为 14.3 min，18.1 min，毛发样品中 AEA、2-AG 的保留 时间分别为 14.0 min，17.7 min。毛发样品中 AEA 的检测限、定量限、回收率为 0.7 pg/mg、2.1 pg/mg 和 97.9%，毛发样品中 2-AG 的检测限、定量限、回收率为 3.2 pg/mg、10.9 pg/mg 和 99.3%，且二者的日内、日间变异系数均低于 15%。癫痫发作 1 级、2 级、3 级、4 级、5 级这五个分级癫痫小鼠的 AEA 含量和 2-AG 含量依次升高，不同癫痫发作分级小鼠毛发样品中 AEA 含量和 2-AG 含量比较 (P 均 <0.05) 。结论：LC-MS/MS 测定癫痫小鼠毛发中 2-AG、AEA 的表达量，具有灵敏度更高，样品使用量更少 等优点，可大规模样本研究。 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
