دورية أكاديمية
The RNA-binding protein Mex3b regulates the spatial organization of the Rap1 pathway
| العنوان: | The RNA-binding protein Mex3b regulates the spatial organization of the Rap1 pathway |
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| المؤلفون: | Le Borgne, Maïlys, Chartier, Nicolas, Buchet-Poyau, Karine, Destaing, Olivier, Faurobert, Eva, Thibert, Chantal, Rouault, Jean-Pierre, Courchet, Julien, Nègre, Didier, Bouvard, Daniel, Albiges-Rizo, Corinne, Rousseaux, Sophie, Khochbin, Saadi, Segretain, Dominique, Crepieux, Pascale, Guillou, Florian Jean Louis, Durand, Philippe, Perrard, Marie-Hélène, Billaud, Marc |
| المساهمون: | Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Pôle Information Médicale Evaluation Recherche (IMER), Hospices Civils de Lyon (HCL), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Neuroscience New York, Columbia University New York, Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (Equipe EVIR), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Physiologie de la reproduction et des comportements Nouzilly (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation Saumur -Institut National de la Recherche Agronomique (INRA), Association pour la Recherche sur le Cancer (ARC) 20110603000, a fellowship from ARC (to M.L.B.), and a fellowship from the Fondation pour la Recherche Médicale (FRM) (to N.C.). |
| المصدر: | ISSN: 0951-1991. |
| بيانات النشر: | HAL CCSD Company of Biologists |
| سنة النشر: | 2014 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | mouse, rap1, sertoli cell, rna binding proteins, mex 3, phagocytosis, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] |
| الوصف: | The four related mammalian MEX-3 RNA-binding proteins are evolutionarily conserved molecules for which the in vivo functions have not yet been fully characterized. Here, we report that male mice deficient for the gene encoding Mex3b are subfertile. Seminiferous tubules of Mex3b-deficient mice are obstructed as a consequence of the disrupted phagocytic capacity of somatic Sertoli cells. In addition, both the formation and the integrity of the blood-testis barrier are compromised owing to mislocalization of N-cadherin and connexin 43 at the surface of Sertoli cells. We further establish that Mex3b acts to regulate the cortical level of activated Rap1, a small G protein controlling phagocytosis and cell-cell interaction, through the activation and transport of Rap1GAP. The active form of Rap1 (Rap1-GTP) is abnormally increased at the membrane cortex and chemically restoring Rap1-GTP to physiological levels rescues the phagocytic and adhesion abilities of Sertoli cells. Overall, these findings implicate Mex3b in the spatial organization of the Rap1 pathway that orchestrates Sertoli cell functions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/24803656; hal-01129838; https://hal.archives-ouvertes.fr/hal-01129838Test; PRODINRA: 262134; PUBMED: 24803656; WOS: 000336126400012 |
| DOI: | 10.1242/dev.108514 |
| الإتاحة: | https://doi.org/10.1242/dev.108514Test https://hal.archives-ouvertes.fr/hal-01129838Test |
| رقم الانضمام: | edsbas.6ABBE27A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/dev.108514 |
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