Coexistence of circulating HBsAg and anti-HBs antibodies in chronic hepatitis B carriers is not a simple analytical artifact and does not influence HBsAg quantification
| العنوان: | Coexistence of circulating HBsAg and anti-HBs antibodies in chronic hepatitis B carriers is not a simple analytical artifact and does not influence HBsAg quantification |
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| المؤلفون: | Thierry Poynard, Sepideh Akhavan, Marie Pancher, Coralie Pallier, Vincent Thibault, Yen Ngo, Nathalie Désiré |
| المساهمون: | CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire de Physiopathologie hépatique (PH), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Service de virologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse, Centre d'Immunologie et de Maladies Infectieuses (CIMI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Administateur, HAL Sorbonne Université, Service de virologie [CHU Pitié-Salpêtrière], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP], Laboratoire de Physiopathologie hépatique ( PH ), Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ), Service d'Hépato-Gastro-Entérologie, CHU Pitié-Salpêtrière [APHP], Assistance Publique - Hôpitaux de Paris, Assistance publique - Hôpitaux de Paris (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Paul Brousse, Centre d'Immunologie et de Maladies Infectieuses ( CIMI ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) |
| المصدر: | Journal of Clinical Virology Journal of Clinical Virology, 2015, 62, pp.32-37. ⟨10.1016/j.jcv.2014.11.015⟩ Journal of Clinical Virology, Elsevier, 2015, 62, pp.32-37. ⟨10.1016/j.jcv.2014.11.015⟩ Journal of Clinical Virology, Elsevier, 2015, 62, pp.32-37. 〈10.1016/j.jcv.2014.11.015〉 |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Anti hbs, HBsAg, Human immunodeficiency virus (HIV), medicine.disease_cause, Serology, 0302 clinical medicine, Genotype, Hbv genotype, Medicine, Aged, 80 and over, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Immune complexes, 0303 health sciences, biology, virus diseases, Middle Aged, 3. Good health, Infectious Diseases, Carrier State, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, 030211 gastroenterology & hepatology, Antibody, Artifacts, Adult, Hepatitis B virus, [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Sensitivity and Specificity, Young Adult, 03 medical and health sciences, Hepatitis B, Chronic, Chronic hepatitis, Virology, Humans, Hepatitis B Antibodies, Aged, 030304 developmental biology, Hepatitis B Surface Antigens, business.industry, Reproducibility of Results, HBsAg quantification, HBsAg variant, digestive system diseases, Immunology, biology.protein, Reagent Kits, Diagnostic, business, Interference |
| الوصف: | International audience; Background : Presence at the same time of HBsAg and anti-HBs antibodies (HBsAg/Ab) is an entity sometimes encountered in chronic hepatitis B (CHB) carriers.Objectives : This study was designed to characterize such serological profiles and to assess the reliability of serological marker quantification by three commercially available assays in this setting.Study design : Among 2578 CHB identified patients, 129 (5%) had an HBsAg/Ab profile as determined by Abbott Architect. After exclusion of co-infections (HIV, HCV, HDV), HBV reactivation or HBIg treatment, 101 samples from 62 patients were tested for HBsAg and anti-HBs quantification using Architect, DiaSorin Liaison-XL and Roche Modular-Cobas. Influence of genotype and HBsAg variants was studied in 31 samples with HBV replication.Results : HBsAg detection was confirmed with the 3 techniques for 98% (n = 99) of the samples while the HBsAg/Ab profile was concordant between all techniques for 65% of them. The overall correlation between the 3 HBsAg quantification techniques was good (R2: 0.94–0.97). The median HBsAg concentration was comparable for the 99 samples whatever the used technique but a bias of −0.11 and 0.02 log IU/mL were noticed for DiaSorin and Roche compared to Abbott, respectively. Anti-HBs quantifications were poorly correlated between techniques with major discrepancies observed. Genotype and substitutions within the “a” determinant showed an impact on HBsAg quantification.Conclusions : The double HBsAg/Ab profile is not an analytical artifact and is confirmed on all commercially available techniques. While such profile does not influence HBsAg quantification, differences of HBsAg quantification were noticed according to HBV genotype or HBsAg variant. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1386-6532 1873-5967 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::306fd07e28eeaf0e84130cb4ed34912bTest https://hal.sorbonne-universite.fr/hal-01102734Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....306fd07e28eeaf0e84130cb4ed34912b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13866532 18735967 |
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