Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility
| العنوان: | Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility |
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| المؤلفون: | Robert A. Nicholas, Frédérique Vernel-Pauillac, Cyrille Goarant, Sobhan Nandi |
| المساهمون: | Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Department of Pharmacology, University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC)-University of North Carolina System (UNC) |
| المصدر: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2008, 52 (9), pp.3293-300. ⟨10.1128/AAC.00020-08⟩ |
| بيانات النشر: | HAL CCSD, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Sexually transmitted disease, Male, MESH: Sequence Analysis, DNA, Antibiotics, medicine.disease_cause, Polymerase Chain Reaction, MESH: Genotype, Gonorrhea, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Genotype, Pharmacology (medical), MESH: Bacterial Proteins, Antibacterial agent, 0303 health sciences, MESH: Microbial Sensitivity Tests, 3. Good health, Anti-Bacterial Agents, Infectious Diseases, MESH: Neisseria gonorrhoeae, MESH: Repressor Proteins, Female, medicine.drug, medicine.drug_class, Penicillin Resistance, Porins, Microbial Sensitivity Tests, Penicillins, Biology, Microbiology, 03 medical and health sciences, Antibiotic resistance, Bacterial Proteins, New Caledonia, MESH: Penicillins, Mechanisms of Resistance, MESH: Anti-Bacterial Agents, medicine, Humans, Genotyping, 030304 developmental biology, Pharmacology, MESH: Porins, MESH: Humans, 030306 microbiology, MESH: Gonorrhea, MESH: Polymerase Chain Reaction, Sequence Analysis, DNA, MESH: Penicillin Resistance, MESH: New Caledonia, Virology, Neisseria gonorrhoeae, MESH: Male, Penicillin, Repressor Proteins, MESH: Female |
| الوصف: | Antibiotic resistance in Neisseria gonorrhoeae continues to be a major concern in public health. Resistance of N. gonorrhoeae bacteria to penicillin G is widespread in most developed countries, which has necessitated a change to newer drugs for treatment of gonococcal infections. Recent reports indicate that resistance to these newer drugs is increasing, highlighting the need for accurate therapeutic recommendations. In some countries or communities, however, N. gonorrhoeae isolates are still susceptible to penicillin, so the use of this antibiotic for single-dose treatments of medically under-resourced patients is beneficial. In order to evaluate the adequacy and sustainability of this treatment approach, we explored the presence and prevalence of chromosomally mediated resistance determinants in N. gonorrhoeae isolates collected from 2005 to 2007 in New Caledonia. We developed two new real-time PCR assays targeting the penB and mtrR determinants, to be used together with a previously described duplex assay targeting the penA and ponA determinants. The results of this study provided evidence that neither the most-common mtrR determinants nor the most-resistance-associated penB alleles are currently circulating in New Caledonia, suggesting that penicillin should still be considered a valuable treatment strategy. Additionally, using our genotyping assay, we observed an unexpected penB genotype at a relatively high frequency that was associated with a decreased susceptibility to penicillin (average MIC, 0.15 μg/ml). Sequencing revealed that this genotype corresponded to an A102S mutation in the penB gene. The molecular tools developed in this study can be used successfully for prospective epidemiological monitoring and surveillance of penicillin susceptibility. |
| اللغة: | English |
| تدمد: | 0066-4804 1098-6596 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ca426df00d0dbe0bc88b7fdd07fc91dTest https://hal-riip.archives-ouvertes.fr/pasteur-00451014Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0ca426df00d0dbe0bc88b7fdd07fc91d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00664804 10986596 |
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