Molecular epidemiology of dengue viruses in three provinces of Lao PDR, 2006-2010
| العنوان: | Molecular epidemiology of dengue viruses in three provinces of Lao PDR, 2006-2010 |
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| المؤلفون: | Saythong Inthalath, Audrey Dubot-Pérès, Phouthalavanh Souvannasing, Koukeo Phommasone, Manivanh Vongsouvath, Géraldine Piorkowski, Raphaëlle Klitting, Paul N. Newton, Cécile Baronti, Mayfong Mayxay, Onanong Sengvilaipaseuth, Bountoy Sibounheuang, Phouvieng Douangdala, Anisone Chanthongthip, Soulignasack Thongpaseuth, Xavier de Lamballerie, Josée Castonguay-Vanier |
| المساهمون: | Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahidol University [Bangkok]-Mahosot Hospital, Nuffield Department of Clinical Medicine [Oxford], University of Oxford, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Health Sciences [Vientiane, Laos] (UHS), Luang Namtha Provincial Hospital, Salavan Provincial Hospital, University of Oxford [Oxford], HAL AMU, Administrateur, Graduate School, AII - Infectious diseases, APH - Global Health, APH - Methodology |
| المصدر: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, 2018, 12 (1), ⟨10.1371/journal.pntd.0006203⟩ PLoS Neglected Tropical Diseases, Public Library of Science, 2018, 12 (1), ⟨10.1371/journal.pntd.0006203⟩ PLoS Neglected Tropical Diseases, Vol 12, Iss 1, p e0006203 (2018) PLoS neglected tropical diseases, 12(1):e0006203. Public Library of Science |
| بيانات النشر: | HAL CCSD, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | RNA viruses, 0301 basic medicine, Serotype, Viral Diseases, Veterinary medicine, viruses, Artificial Gene Amplification and Extension, Dengue virus, Pathology and Laboratory Medicine, medicine.disease_cause, Polymerase Chain Reaction, Dengue Fever, Dengue fever, Dengue, Database and Informatics Methods, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Epidemiology, Medicine and Health Sciences, Ethnicities, Child, Data Management, Molecular Epidemiology, Denv serotypes, lcsh:Public aspects of medicine, virus diseases, Phylogenetic Analysis, Middle Aged, 3. Good health, Phylogenetics, Phylogeography, Infectious Diseases, Laos, Medical Microbiology, Child, Preschool, Viral Pathogens, Epidemiological Monitoring, Viruses, Lao People, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Topography, Medical, Seasons, Pathogens, Sequence Analysis, Research Article, Neglected Tropical Diseases, Adult, Computer and Information Sciences, medicine.medical_specialty, lcsh:Arctic medicine. Tropical medicine, Adolescent, Bioinformatics, lcsh:RC955-962, 030231 tropical medicine, Sequence Databases, Biology, Serogroup, Research and Analysis Methods, Microbiology, Young Adult, 03 medical and health sciences, Extraction techniques, medicine, Humans, Evolutionary Systematics, Molecular Biology Techniques, Microbial Pathogens, Molecular Biology, Taxonomy, Evolutionary Biology, Biology and life sciences, Flaviviruses, Molecular epidemiology, Organisms, Public Health, Environmental and Occupational Health, Outbreak, lcsh:RA1-1270, Reverse Transcriptase-Polymerase Chain Reaction, Dengue Virus, biochemical phenomena, metabolism, and nutrition, Tropical Diseases, medicine.disease, RNA extraction, Biological Databases, 030104 developmental biology, People and Places, Population Groupings, Sequence Alignment |
| الوصف: | Few data on dengue epidemiology are available for Lao PDR. Here, we provide information on the complexity of dengue epidemiology in the country, demonstrating dynamic circulation that varies over space and time, according to serotype. We recruited 1,912 consenting patients presenting with WHO dengue criteria at Mahosot Hospital, Vientiane (central Laos), between 2006 and 2010. Between 2008 and 2010, 1,413 patients with undifferentiated fever were also recruited at Luang Namtha (LNT) Provincial Hospital (northern Laos) and 555 at Salavan (SV) Provincial Hospital (southern Laos). We report significant variations in Dengue virus (DENV) circulation between the three sites. Peaks of DENV infection were observed in the rainy seasons, although 11% of confirmed cases in the provinces and 4.6% in the capital were detected during the dry and cool seasons (between December and February). Four DENV serotypes were detected among the 867 RT-PCR positive patients: 76.9% DENV-1, 9.6% DENV-2, 7.7% DENV-4 and 5.3% DENV-3. DENV-1 was the predominant serotype throughout the study except in LNT in 2008 and 2009 when it was DENV-2. Before July 2009, DENV-2 was not detected in SV and only rarely detected in Vientiane. DENV-3 and DENV-4 were commonly detected in Vientiane, before 2008 for DENV-4 and after 2009 for DENV-3. The phylogenetic analyses of DENV envelope sequences suggest concurrent multiple introductions of new strains as well as active DENV circulation throughout Laos and with neighboring countries. It is therefore of great importance to develop and strengthen a year-round nation-wide surveillance network in order to collect data that would allow anticipation of public health issues caused by the occurrence of large dengue outbreaks. Author summary Dengue is a mosquito-borne disease that can be caused by 4 viruses. It is a flu-like disease but can sometime be more severe and cause hemorrhage or death. An estimated 390 million people are infected every year, mainly in the Western Pacific and in South-East Asia. In Laos, where our study was conducted from 2006 to 2010, little was known on the circulation of the different dengue viruses. A total of 1,912 patients were recruited at Mahosot Hospital, Vientiane (central Laos), 1,413 patients at Luang Namtha Provincial Hospital (northern Laos) and 555 at Salavan Provincial Hospital (southern Laos). Although most Dengue virus infections were detected during rainy seasons (where mosquitoes are the most active) in all three provinces, some patients were also infected during the dryer months. All 4 dengue viruses were detected with different distributions: mostly type 1 in the capital and in the South, and type 1 and 2 in equal proportion in the north. Type 3 and 4 were not detected in Luang Namtha and rarely in Salavan. Comparison of Dengue virus sequences from Laos with sequences collected worldwide showed an active year-round circulation of dengue within Laos and with neighboring countries. It is hence of great importance to develop and strengthen a year-round nation-wide surveillance network in order to collect data that would allow anticipation of public health issues caused by the occurrence of large dengue outbreaks. |
| وصف الملف: | application/pdf; text/pdf |
| اللغة: | English |
| تدمد: | 1935-2727 1935-2735 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57e83746fd48facd9e1112f95108120cTest https://hal.science/hal-01765838Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57e83746fd48facd9e1112f95108120c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19352727 19352735 |
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