رسالة جامعية
Role of CD169 macrophages in cellular adaptive immune responses ; Rôle des macrophages CD169 dans les réponses immunitaires adaptatives cellulaires
| العنوان: | Role of CD169 macrophages in cellular adaptive immune responses ; Rôle des macrophages CD169 dans les réponses immunitaires adaptatives cellulaires |
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| المؤلفون: | Mauvais, Francois-Xavier |
| المساهمون: | Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité, Peter Van Endert |
| المصدر: | https://theses.hal.science/tel-04515492Test ; Immunology. Université Sorbonne Paris Cité, 2016. English. ⟨NNT : 2016USPCB259⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | CD169 macrophages, Dendritic cells, Endosome, Phagosome, MHC-I, Adaptive immunity, Antigen cross-presentation, Spleen, Macrophages CD169, Cellules dendritiques, Immunité adaptative, Présentation croisée, Rate, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | Protective immunity against infections requires the initiation of adaptive immune responses, including the generation of cytotoxic T lymphocytes specific of antigenic motifs expressed by microbes. These processes have to be tightly regulated to avoid the persistence of inflammation and the development of autoimmunity. In this context, dendritic cells play a major role and can efficiently present exogenous antigenic material for loading onto the major histocompatibility class II molecules (MHC-II), but also MHC-I molecules in a process referred to as cross-presentation. However, it is not clear if other phagocytes have similar capacities. For example, the ability of CD169+ macrophages to present antigens to T cells is controversial: in the lymph nodes they generate protective immunity against tumors by cross-presenting tumor derived antigens, whereas in the spleen they may act as an antigen transfer platform for cross-presenting dendritic cells. The crucial barrier to determining their precise role in adaptive immunity is the difficulty to isolate them. The objectives of this work are to: clarify the contribution of splenic CD169+ macrophages in inducing adaptive immune responses; identify the molecular and cellular factors involved in the regulation of innate and adaptive immunity by phagocyte subsets. By developing a unique strategy that combines density gradients and cell sorting by flow cytometry, we showed that CD169+ cells have a molecular and protein signatures close to macrophages. We place the splenic CD169+ macrophages together with dendritic cells, in a group of professional antigen presenting cells capable of efficient cross-presentation. Following antigen targeting to splenic CD169+ macrophages, specific naïve CD8+ T cells form long-standing contacts with macrophages resulting in their activation and differentiation into effector and memory cells. Surprisingly, we found that CD169+ macrophages exclusively use a unique vacuolar antigen cross-presentation pathway to generate antigenic peptides for ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| العلاقة: | NNT: 2016USPCB259; tel-04515492; https://theses.hal.science/tel-04515492Test; https://theses.hal.science/tel-04515492/documentTest; https://theses.hal.science/tel-04515492/file/va_Mauvais_Francois-Xavier.pdfTest |
| الإتاحة: | https://theses.hal.science/tel-04515492Test https://theses.hal.science/tel-04515492/documentTest https://theses.hal.science/tel-04515492/file/va_Mauvais_Francois-Xavier.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.181A9B0F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |