Chyme reinfusion restores the regulatory bile salt-FGF19 axis in intestinal failure patients
| العنوان: | Chyme reinfusion restores the regulatory bile salt-FGF19 axis in intestinal failure patients |
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| المؤلفون: | Koelfat, Kiran V K, Picot, Denis, Chang, Xinwei, Desille, Mireille, van Eijk, Hans M, van Kuijk, Sander M J, Lenicek, Martin, Layec, Sabrina, Carsin, Marie, Dussaulx, Laurence, Seynhaeve, Eloi, Trivin, Florence, Lacaze, Laurence, Thibault, Ronan, Schaap, Frank G, Olde Damink, Steven W M |
| المساهمون: | School of Nutrition and Translational Research in Metabolism [Maastricht] (NUTRIM), Maastricht University [Maastricht], Clinique Saint-Yves [Rennes], Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Maastricht University Medical Centre (MUMC), Charles University [Prague] (CU), CHU Pontchaillou [Rennes], Universitätsklinikum RWTH Aachen - University Hospital Aachen [Aachen, Germany] (UKA), RWTH Aachen University, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), 2015‐16, 022.003.011, 2014‐15, Jonchère, Laurent |
| المصدر: | Hepatology Hepatology, Wiley-Blackwell, 2021, 74 (5), pp.2670-2683. ⟨10.1002/hep.32017⟩ Hepatology, Wiley-Blackwell, 2021, ⟨10.1002/hep.32017⟩ Hepatology, 2021, 74 (5), pp.2670-2683. ⟨10.1002/hep.32017⟩ |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | [SDV] Life Sciences [q-bio], gut-liver axis, intestinal failure, [SDV]Life Sciences [q-bio], bile salts, FGF19 |
| الوصف: | International audience; Background and aims - Automated chyme reinfusion (CR) in patients with intestinal failure (IF) and a temporary double enterostomy (TDE) restores intestinal function and protects against liver injury, but the mechanisms are incompletely understood. The aim was to investigate whether the beneficial effects of CR relate to functional recovery of enterohepatic signaling through the bile salt-FGF19 axis. Approach and results - Blood samples were collected from 12 patients, 3 days before, at start, and 1, 3, 5, and 7 weeks after CR initiation. Plasma FGF19, total bile salts (TBS), 7-α-hydroxy-4-cholesten-3-one (C4; a marker of bile salt synthesis), citrulline (CIT), bile salt composition, liver tests, and nutritional risk indices were determined. Paired small bowel biopsies prior to CR and after 21 days were taken, and genes related to bile salt homeostasis and enterocyte function were assessed. CR induced an increase in plasma FGF19 and decreased C4 levels, indicating restored regulation of bile salt synthesis through endocrine FGF19 action. TBS remained unaltered during CR. Intestinal farnesoid X receptor was up-regulated after 21 days of CR. Secondary and deconjugated bile salt fractions were increased after CR, reflecting restored microbial metabolism of host bile salts. Furthermore, CIT and albumin levels gradually rose after CR, while abnormal serum liver tests normalized after CR, indicating restored intestinal function, improved nutritional status, and amelioration of liver injury. CR increased gene transcripts related to enterocyte number, carbohydrate handling, and bile salt homeostasis. Finally, the reciprocal FGF19/C4 response after 7 days predicted the plasma CIT time course. Conclusions - CR in patients with IF-TDE restored bile salt-FGF19 signaling and improved gut-liver function. Beneficial effects of CR are partly mediated by recovery of the bile salt-FGF19 axis and subsequent homeostatic regulation of bile salt synthesis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0270-9139 1527-3350 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::bf5d108189ae334b0e717b62ed05de18Test https://hal.archives-ouvertes.fr/hal-03282843/documentTest |
| رقم الانضمام: | edsair.dedup.wf.001..bf5d108189ae334b0e717b62ed05de18 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02709139 15273350 |
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