Effect of water availability on changes in root amino acids and associated rhizosphere on root exudation of amino acids in Pisum sativum L
| العنوان: | Effect of water availability on changes in root amino acids and associated rhizosphere on root exudation of amino acids in Pisum sativum L |
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| المؤلفون: | Anis M. Limami, Caroline Cukier, Richard J. Robins, Joëlle Fustec, Hélène Bobille |
| المساهمون: | Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), USC LEVA - SFR 4207 QUASAV, Ecole supérieure d'Agricultures d'Angers (ESA), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), French Region Pays-de-la-Loire, project 'RHIZOSFER' (2012-09695 ), French Region Pays-de-la-Loire, project 'PLAISIR' Association for International Structure on 'Isotopomic Research' French Regional International Strategy Grant |
| المصدر: | Phytochemistry Phytochemistry, 2019, 161, pp.75-85. ⟨10.1016/j.phytochem.2019.01.015⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Water stress, Plant Science, Horticulture, Plant Roots, 01 natural sciences, Biochemistry, Pisum, Leguminoseae, Sativum, Botany, [CHIM]Chemical Sciences, 15N-labelling, Molecular Biology, 2. Zero hunger, Abiotic component, chemistry.chemical_classification, Rhizobium leguminosarum, Rhizosphere, biology, 010405 organic chemistry, Chemistry, Peas, Water, food and beverages, General Medicine, Metabolism, 15. Life on land, biology.organism_classification, 6. Clean water, 0104 chemical sciences, Amino acid, Shoot, Amino acids, Rhizobium, CG-MS, 010606 plant biology & botany |
| الوصف: | Root exudation is considered to regulate the abundance of the microbial community. It may vary both qualitatively and quantitatively in response to the environment in which the plant is growing. A part of exuded N derives from amino acids (AAs). This, in turn, may help plants to cope with abiotic stresses by favouring positive interactions with the rhizosphere environment, thus playing a potential role in maintaining healthy plants. In this respect, an under-investigated area is the effect of stress due to water deficit (WD). It is proposed that the AA profile in the rhizosphere may be altered by WD, reflecting a modulation of root AA exudation linked to a physiological response of the plant to water stress. To investigate this, Pisum sativum L. plants, grown in unsterilised Rhizobium leguminosarum-enriched soil, were stem-labelled with N-15-urea for 96 h, and then subjected/not subjected to 72 h of WD. The concentrations and abundance of N-15-labelling in individual AAs were determined in both roots and the associated rhizosphere at 24,48 and 72 h after stress application. It was found that both AAs metabolism in the pea root and AAs exudation were strongly modified in WD conditions. After 24 h of WD, the concentrations of all measured AAs increased in the roots, accompanied by a dramatic stress-related increase in the N-15-labelling of some AAs. Furthermore, after 48-72 h of WD, the concentrations of Pro, Ala and Glu increased significantly within the rhizosphere, notably with a concomitant increase in N-15-enrichment in Pro, Ser, Asn, Asp, Thr and Ile. These results support the concept that, in response to WD, substantial amounts of recently assimilated N are rapidly translocated from the shoots to the roots, a portion of which is exuded as AAs. This leads to the rhizosphere being relatively augmented by specific AAs (notably HSer, Pro and Ala) in WD conditions, with a potential impact on soil water retention. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f09b8d9473529d38cb2a85dd4581257Test https://hal.archives-ouvertes.fr/hal-02140854Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2f09b8d9473529d38cb2a85dd4581257 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |