دورية أكاديمية
Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum
| العنوان: | Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum |
|---|---|
| المؤلفون: | Jabeena, C. A., Govindaraju, Gayathri, Rawat, Mukul, Gopi, Soundhararajan, Sethumadhavan, Devadathan Valiyamangalath, Jaleel, Abdul, Sasankan, Dhakshmi, Karmodiya, Krishanpal, Rajavelu, Arumugam |
| المصدر: | Jabeena , C A , Govindaraju , G , Rawat , M , Gopi , S , Sethumadhavan , D V , Jaleel , A , Sasankan , D , Karmodiya , K & Rajavelu , A 2021 , ' Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum ' , Journal of Biological Chemistry , vol. 296 , 100614 . https://doi.org/10.1016/j.jbc.2021.100614Test |
| سنة النشر: | 2021 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | /dk/atira/pure/subjectarea/asjc/1300/1303, name=Biochemistry, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1307, name=Cell Biology |
| الوصف: | Epigenetic modifications have emerged as critical regulators of virulence genes and stage-specific gene expression in Plasmodium falciparum. However, the specific roles of histone core epigenetic modifications in regulating the stage-specific gene expression are not well understood. In this study, we report an unconventional trimethylation at lysine 64 on histone 3 (H3K64me3) and characterize its functional relevance in P. falciparum. We show that PfSET4 and PfSET5 proteins of P. falciparum methylate H3K64 and that they prefer the nucleosome as a substrate over free histone 3 proteins. Structural analysis of PfSET5 revealed that it interacts with the nucleosome as a dimer. The H3K64me3 mark is dynamic, being enriched in the ring and trophozoite stages and drastically reduced in the schizont stages. Stage-specific global chromatin immunoprecipitation -sequencing analysis of the H3K64me3 mark revealed the selective enrichment of this methyl mark on the genes of exported family proteins in the ring and trophozoite stages and a significant reduction of the same in the schizont stages. Collectively, our data identify a novel epigenetic mark that is associated with the subset of genes encoding for exported proteins, which may regulate their expression in different stages of P. falciparum. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/f42253e5-c81d-436c-a490-9af39d796766Test |
| DOI: | 10.1016/j.jbc.2021.100614 |
| الإتاحة: | https://doi.org/10.1016/j.jbc.2021.100614Test https://discovery.dundee.ac.uk/en/publications/f42253e5-c81d-436c-a490-9af39d796766Test https://discovery.dundee.ac.uk/ws/files/115069538/PIIS0021925821003951.pdfTest http://www.scopus.com/inward/record.url?scp=85104656047&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D907BDED |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jbc.2021.100614 |
|---|