المؤلفون: Tebeje, Biniam, Harvie, Marina, You, Hong, Loukas, Alex, McManus, Donald

مصطلحات موضوعية: Schistosoma mansoni, Schistosoma haematobium, Schistosoma japonicum, Immune response, Schistosomiasis, Vaccine, Immune protection, Antigen discovery

الوصف: Schistosomiasis, caused mainly by S. mansoni , S. haematobium and S. japonicum, continues to be a serious tropical disease and public health problem resulting in an unacceptably high level of morbidity in countries where it is endemic. Praziquantel, the only drug currently available for treatment, is unable to kill developing schistosomes, it does not prevent re-infection and its continued extensive use may result in the future emergence of drug-resistant parasites. This scenario provides impetus for the development and deployment of anti-schistosome vaccines to be used as part of an integrated approach for the prevention, control and eventual elimination of schistosomiasis. This review considers the present status of candidate vaccines for schistosomiasis, and provides some insight on future vaccine discovery and design.

العلاقة: http://www.parasitesandvectors.com/content/9/1/528Test

الإتاحة: http://www.parasitesandvectors.com/content/9/1/528Test

المؤلفون: Driguez, Patrick, Doolan, Denise L, Loukas, Alex, Felgner, Philip L, McManus, Donald P

الوصف: The recent publication of the Schistosoma japonicum and S. mansoni genomes has expanded greatly the opportunities for post-genomic schistosomiasis vaccine research. Immunomics protein microarrays provide an excellent application of this new schistosome sequence information, having been utilised successfully for vaccine antigen discovery with a range of bacterial and viral pathogens, and malaria. Accordingly, we have designed and manufactured a Schistosoma immunomics protein microarray as a vaccine discovery tool. The microarray protein selection combined previously published data and in silico screening of available sequences for potential immunogens based on protein location, homology to known protective antigens, and high specificity to schistosome species. Following cloning, selected sequences were expressed cell-free and contact-printed onto nitrocellulose microarrays. The reactivity of microarray proteins with antisera from schistosomiasis-exposed/resistant animals or human patients can be measured with labelled secondary antibodies and a laser microarray scanner; highly reactive proteins can be further assessed as putative vaccines. This highly innovative technology has the potential to transform vaccine research for schistosomiasis and other parasitic diseases of humans and animals.

العلاقة: http://www.parasitesandvectors.com/content/3/1/4Test

الإتاحة: http://www.parasitesandvectors.com/content/3/1/4Test