مؤتمر
Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study ; Le Cortisol et la DHEA comme marqueurs de la placentite chez la jument gestante : une étude préliminaire
| العنوان: | Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study ; Le Cortisol et la DHEA comme marqueurs de la placentite chez la jument gestante : une étude préliminaire |
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| المؤلفون: | Ledeck, Joy, Peeters, Stéphanie, Le Goff, Caroline, Deleuze, Stefan, Cavalier, Etienne, Ponthier, Jérôme |
| المساهمون: | FARAH. Médecine vétérinaire comparée - ULiège, FARAH - Fundamental and Applied Research for Animals and Health - ULiège |
| المصدر: | Journal of Equine Veterinary Science, 125 (2023-06); International Symposium On Equine Reproduction, Foz do Iguaçu, Brazil [BR], 10-14 juillet 2023 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Cortisol, DHEA, Placentitis, Mares, Liquid chromatography coupled to mass spectrometry, Biomarkers, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | peer reviewed ; Placentitis in mares is a subclinical cause of abortion, high- lighting the importance of early detection for successful treatment. Sexual steroids could serve as biomarkers, but cross-reactivity and a limited detection range of immunoassays may hinder ac- curate measures. This study evaluated some steroids as poten- tial markers of placentitis using liquid chromatography coupled to mass spectrometry (LC-MS), known to improve detection and quantification of steroids with good selectivity, specificity, and allowing multiplexing capabilities (Conley et al., Reproduction. 2019;158:197-208; Ledeck et al., Theriogenology. 2022;189:86- 91). From the 7th month of pregnancy, mares were transrectally scanned to measure the combined thickness of the uterus and placenta (CTUP), and blood samples were collected at a fixed time of day. Ten healthy mares (HM) and nine mares diagnosed with non-experimentally-induced placentitis (PM) between 8 and 10 months were enrolled, based on ultrasonographic placentitis signs: heterogeneous echogenicity and/or thickened CTUP. Post- partum allantochorion examination confirmed the diagnosis. Sub- sequently, PM were further excluded from the study as they re- ceived treatments for placentitis. Serum concentrations of pro- gesterone, 17α-hydroxyprogesterone (17αOHP), dehydroepiandros- terone (DHEA), and cortisol were assayed using LC-MS. Non- normally distributed data were presented as medians, and groups were compared at the same month of pregnancy using the Mann- Whitney test. There were no significant differences in 17αOHP and progesterone concentrations between groups at any month. DHEA concentrations were significantly higher (p=0.0297) at 8 months in PM (3.552μg/L) compared to HM (2.240μg/L). A sim- ilar trend was observed at 9 months, with DHEA concentra- tions of 2.126μg/L and 1.445μg/L in PM and HM, respectively (p=0.0604). In contrast, HM had significantly higher (p=0.022) cortisol concentrations at 7 months (71.75μg/L) compared to PM (38.62μg/L). This difference ... |
| نوع الوثيقة: | conference object report |
| اللغة: | English |
| تدمد: | 0737-0806 1542-7412 |
| العلاقة: | Cortisol and DHEA as markers of placentitis in pregnant mares: a preliminary study; urn:issn:0737-0806; urn:issn:1542-7412; https://orbi.uliege.be/handle/2268/305667Test; info:hdl:2268/305667; https://orbi.uliege.be/bitstream/2268/305667/1/Ledeck%20Joy%20ISER%20XIII.pdfTest |
| DOI: | 10.1016/j.jevs.2023.104773 |
| الإتاحة: | https://doi.org/10.1016/j.jevs.2023.104773Test https://orbi.uliege.be/handle/2268/305667Test https://orbi.uliege.be/bitstream/2268/305667/1/Ledeck%20Joy%20ISER%20XIII.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DD741A8C |
| قاعدة البيانات: | BASE |
| تدمد: | 07370806 15427412 |
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| DOI: | 10.1016/j.jevs.2023.104773 |