دورية أكاديمية
One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography
| العنوان: | One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography |
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| المؤلفون: | Shi, Junyou, Li, Guoliang, Wang, Honglun, Zheng, Jie, Suo, Yourui, You, Jinmao, Liu, Yongjun |
| سنة النشر: | 2011 |
| المجموعة: | Northwest Institute of Plateau Biology: NWIPB OpenIR (Chinese Academy of Sciences) / 中国科学院西北高原生物研究所机构知识库 |
| مصطلحات موضوعية: | High-speed Counter-current Chromatography, Poacynum Hendersonii, Isoquercitrin, Quercetin-3-o-sophoroside, Quercetin-3-o-(6 ''-o-malonyl)-beta-d-glucoside, Science & Technology, Life Sciences & Biomedicine, Physical Sciences, APOCYNUM-VENETUM LEAVES, AQUEOUS EXTRACTS, NELUMBO-NUCIFERA, PURIFICATION, RATS, CONSTITUENTS, Biochemistry & Molecular Biology, Plant Sciences, Chemistry, Biochemical Research Methods, Analytical |
| الوصف: | Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. Objective - To develop an efficient method for the preparative isolation and purification of flavonoids from the leaves of Poacynum hendersonii by high-speed counter-current chromatography (HSCCC). Methodology - Powdered Poacynum hendersonii lead was extracted three times with 75% ethanol at 60 degrees C for 3 h. The distribution constant (K(D)) was measured to select an optimal two-phase solvent system for HSCCC separation. The purities of the target compounds were tested using HPLC and their structures were identified by (1)H-NMR and (13)C-NMR. Results - Using a two-phase solvent system composed of n-butanol-petroleum ether-0.5% acetic acid (5:3:5, v/v), three main flavonoids, i.e. isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside, were separated from 240 mg crude sample in a one-step separation by using HSCCC method. After further purification with a Sepdex-LH20 column, 5.7 mg isoquercitrin (LC purity 98.72%), 4.9 mg quercetin-3-O-sophoroside (LC purity 99.06%) and 7.4 mg quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside (LC purity 99.31%) were obtained, respectively. Conclusion - The optimised high-speed counter-current chromatography method is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii. Copyright (C) 2011 John Wiley & Sons, Ltd. ; Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. |
| نوع الوثيقة: | article in journal/newspaper report |
| اللغة: | English |
| العلاقة: | PHYTOCHEMICAL ANALYSIS; Shi, JY; Li, GL; Wang, HL; Zheng, J; Suo, YR; You, JM; Liu, YJ.One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2011,22(5):450-454; http://ir.nwipb.ac.cn//handle/363003/1512Test |
| الإتاحة: | http://ir.nwipb.ac.cn//handle/363003/1512Test |
| رقم الانضمام: | edsbas.5731DD69 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |