تقرير
Interrogating Metabolic Interactions Between Skeletal Muscle and Liver Circadian Clocks In Vivo
| العنوان: | Interrogating Metabolic Interactions Between Skeletal Muscle and Liver Circadian Clocks In Vivo |
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| المؤلفون: | Smith, Jacob G., Koronowski, Kevin B., Sato, Tomoki, Greco, Carolina, Petrus, Paul, Verlande, Amandine, Chen, Siwei, Samad, Muntaha, Deyneka, Ekaterina, Mathur, Lavina, Blazev, Ronnie, Molendijk, Jeffrey, Mortimer, Thomas, Kumar, Arun, Deryagin, Oleg, Vaca-Dempere, Mireia, Liu, Peng, Orlando, Valerio, Parker, Benjamin L., Baldi, Pierre, Welz, Patrick-Simon, Jang, Cholsoon, Masri, Selma, Benitah, Salvador Aznar, Muñoz-Cánoves, Pura, Sassone-Corsi, Paolo |
| المساهمون: | King Abdullah University of Science and Technology, KAUST Environmental Epigenetics Research Program, Biological and Environmental Sciences and Engineering Division, Thuwal 23955, Saudi Arabia, Biological and Environmental Science and Engineering (BESE) Division, Bioscience Program |
| بيانات النشر: | Cold Spring Harbor Laboratory |
| سنة النشر: | 2022 |
| المجموعة: | King Abdullah University of Science and Technology: KAUST Repository |
| الوصف: | Expressed throughout the body, the circadian clock system achieves daily metabolic homeostasis at every level of physiology, with clock disruption associated with metabolic disease (1, 2). Molecular clocks present in the brain, liver, adipose, pancreas and skeletal muscle each contribute to glucose homeostasis (3). However, it is unclear; 1) which organ clocks provide the most essential contributions, and 2) if these contributions depend on inter-organ communication. We recently showed that the liver clock alone is insufficient for most aspects of daily liver glucose handling and requires connections with other clocks (4). Considering the pathways that link glucose metabolism between liver and skeletal muscle, we sought to test whether a clock connection along this axis is important. Using our previous published methodology for tissue-specific rescue of Bmal1 in vivo (4, 5), we now show that in the absence of feeding-fasting cycles, liver and muscle clocks are not sufficient for systemic glucose metabolism, nor do they form a functional connection influencing local glucose handling or daily transcriptional rhythms in each tissue. However, the introduction of a daily feeding-fasting rhythm enables a synergistic state between liver and muscle clocks that leads to restoration of systemic glucose tolerance. These findings reveal limited autonomous capabilities of liver and muscle clocks and highlight the need for inter-organ clock communication for glucose homeostasis which involves at least two peripheral metabolic organs. ; This paper is dedicated to the memory of Professor Paolo Sassone-Corsi, a hugely inspiring scientist and mentor and who remains an important influence on our work. We extend special thanks to our animal technician S. Sato and laboratory manager W. Orquiz for valuable help, as well as to all members of the P.S.C. laboratory for support. J.S. was supported by Zymo-CEM Postdoctoral Fellowship (Zymo Research) awarded at the University of California, Irvine. K.B.K. is supported by NIH, NIDDK F32 ... |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | Smith, J. G., Koronowski, K. B., Sato, T., Greco, C., Petrus, P., Verlande, A., Chen, S., Samad, M., Deyneka, E., Mathur, L., Blazev, R., Molendijk, J., Mortimer, T., Kumar, A., Deryagin, O., Vaca-Dempere, M., Liu, P., Orlando, V., Parker, B. L., … Sassone-Corsi, P. (2022). Interrogating Metabolic Interactions Between Skeletal Muscle and Liver Circadian Clocks In Vivo. https://doi.org/10.1101/2022.02.27.482160Test; http://hdl.handle.net/10754/686307Test |
| DOI: | 10.1101/2022.02.27.482160 |
| الإتاحة: | https://doi.org/10.1101/2022.02.27.482160Test http://hdl.handle.net/10754/686307Test |
| حقوق: | This is a preprint version of a paper and has not been peer reviewed. Archived with thanks to Cold Spring Harbor Laboratory. |
| رقم الانضمام: | edsbas.D72D0664 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2022.02.27.482160 |
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