صورة
Image_1_The PolS-PolR Two-Component System Regulates Genes Involved in Poly-P Metabolism and Phosphate Transport in Microlunatus phosphovorus.JPEG
| العنوان: | Image_1_The PolS-PolR Two-Component System Regulates Genes Involved in Poly-P Metabolism and Phosphate Transport in Microlunatus phosphovorus.JPEG |
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| المؤلفون: | Chuanqing Zhong, Peipei Zhang, Cheng Liu, Meng Liu, Wenbing Chen, Jiafang Fu, Xiaoyu Qi, Guangxiang Cao |
| سنة النشر: | 2019 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Microbiology, Microbial Genetics, Microbial Ecology, Mycology, polyphosphate metabolism, two-component system, gene regulation, Microlunatus phosphovorus, phosphate transport |
| الوصف: | Microlunatus phosphovorus NM-1 is a polyphosphate (poly-P)-accumulating bacterium that accumulates poly-P under aerobic conditions and degrades poly-P under anaerobic conditions. In this study, the two-component system (TCS) PolS-PolR was identified in NM-1, and the response regulator PolR was found to directly bind to the promoters of genes related to phosphate transport (MLP_RS00235, MLP_RS23035, and MLP_RS24590); poly-P catabolism (MLP_RS12905) and poly-P synthesis (MLP_RS23025). RT-qPCR assays showed that ppgk (MLP_RS12905), ppk (MLP_RS23025), pstS (MLP_RS23035), and pit (MLP_RS24590) were down-regulated during the aerobic-anaerobic shift. The sequence GTTCACnnnnnGTTCaC was identified as a recognition sequence for PolR by MEME analysis and DNase I footprinting. EMSAs and ChIP-qPCR assays indicated that PolR binds to the promoters of pit (MLP_RS00235), ppgk (MLP_RS12905), ppk (MLP_RS23025), pstS (MLP_RS23035) and pit (MLP_RS24590), and ChIP-qPCR further suggested that the binding affinity of PolR was lower under anaerobic conditions than under aerobic conditions in vivo. These findings indicate that the PolS-PolR TCS in M. phosphovorus may be involved in the regulation of poly-P metabolism in response to levels of dissolved oxygen in the environment, and our results provide insights into new approaches for understanding the mechanisms of phosphorus accumulation and release. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/Image_1_The_PolS-PolR_Two-Component_System_Regulates_Genes_Involved_in_Poly-P_Metabolism_and_Phosphate_Transport_in_Microlunatus_phosphovorus_JPEG/9825266Test |
| DOI: | 10.3389/fmicb.2019.02127.s001 |
| الإتاحة: | https://doi.org/10.3389/fmicb.2019.02127.s001Test https://figshare.com/articles/figure/Image_1_The_PolS-PolR_Two-Component_System_Regulates_Genes_Involved_in_Poly-P_Metabolism_and_Phosphate_Transport_in_Microlunatus_phosphovorus_JPEG/9825266Test |
| حقوق: | CC BY 4.0 |
| رقم الانضمام: | edsbas.224E0646 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2019.02127.s001 |
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