-
1صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: Chosen phages were validated for specificity by indirect ELISA using individual IgG samples purified from OHC (n = 10) and NT1 serum (n = 10). Error bars indicate mean +/- SD, the paired t-test p values has been used to assess statistical significance.
الإتاحة: https://doi.org/10.1371/journal.pone.0297625.g004Test
https://figshare.com/articles/figure/Binding_of_each_phage_clone_toward_individual_serum_IgG_from_10_NT1_and_10_OHC_/25344322Test -
2صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: A. The white bars indicate the input number of random peptide-displayed phage particles in each round of panning, which is persistently kept at 3x10 9 PFU. The black bars show phage particle titration at the ends of each round before they were amplified for the next round of input. B. Increased phage affinity against IgG OHC and NT1 pooled serum after each round of panning. The binding capacity of enriched phage pools to patient IgGs was measured by phage Elisa. The presenting data are mean ± SD (n = 2).
الإتاحة: https://doi.org/10.1371/journal.pone.0297625.g002Test
https://figshare.com/articles/figure/The_persistence_of_phage_output_after_each_round_of_panning_/25344316Test -
3صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: The positive selection (the 1st, 2nd, 3 rd , and 5th rounds) and negative selection (the 4th round) were performed against NT1 and OHC pooled serum IgG, respectively.
الإتاحة: https://doi.org/10.1371/journal.pone.0297625.g001Test
https://figshare.com/articles/figure/Schematic_representation_of_the_phage_display_panning_procedure_/25344313Test -
4صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: After five rounds of positive and negative biopanning, 101 randomly selected phage clones were evaluated for binding to IgG from pooled OHC and NT1 serum via phage ELISA (n = 3). The binding of phage with OHC pooled serum-derived and NT1 pooled serum-derived IgG are analyzed by multiple unpaired t-tests with p<0.05.
العلاقة: https://figshare.com/articles/figure/The_binding_of_101_phages_with_OHC_and_NT1_sera_/25344319Test
الإتاحة: https://doi.org/10.1371/journal.pone.0297625.g003Test
https://figshare.com/articles/figure/The_binding_of_101_phages_with_OHC_and_NT1_sera_/25344319Test -
5صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: Each dot represents an individual sample from the eight patients with other hypersomnolence complaints (OHC) and twenty-six patients with Narcolepsy type I (NT1). Multiple t-test p values are reported for the group comparison between OHC and NT1.
الإتاحة: https://doi.org/10.1371/journal.pone.0297625.g005Test
https://figshare.com/articles/figure/Reactivity_of_serum_antibody_against_the_selected_peptides_assessed_by_ELISA_/25344325Test -
6صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: After five rounds of positive and negative biopanning, 101 randomly selected phage clones were evaluated for binding to IgG from pooled OHC and NT1 serum via phage ELISA (n = 3). The binding of phage with OHC pooled serum-derived and NT1 pooled serum-derived IgG are analyzed by multiple unpaired t-tests with p<0.05.
العلاقة: https://figshare.com/articles/figure/The_binding_of_101_phages_with_OHC_and_NT1_sera_/25344319Test
-
7صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: Chosen phages were validated for specificity by indirect ELISA using individual IgG samples purified from OHC (n = 10) and NT1 serum (n = 10). Error bars indicate mean +/- SD, the paired t-test p values has been used to assess statistical significance.
-
8صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: The positive selection (the 1st, 2nd, 3 rd , and 5th rounds) and negative selection (the 4th round) were performed against NT1 and OHC pooled serum IgG, respectively.
-
9صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: Each dot represents an individual sample from the eight patients with other hypersomnolence complaints (OHC) and twenty-six patients with Narcolepsy type I (NT1). Multiple t-test p values are reported for the group comparison between OHC and NT1.
-
10صورة
المؤلفون: Thi-Tuyet Trinh Tran, Thi-Hong Nhung Nguyen, Yves Dauvilliers, Roland Liblau, Xuan-Hung Nguyen
مصطلحات موضوعية: Neuroscience, Ecology, Immunology, Cancer, Infectious Diseases, Virology, neurons producing orexin, narcolepsy type 1, mediated immunity rather, differential binding capacity, also named hypocretin, serum immunoglobin g, nt1 patient cohort, unbiased random peptide, specific antigens targeted, ohc control group, mer peptides derived, nt1 serum igg, targeted methodologies, specific autoantibodies, various peptides, statistically significant, selective loss, selected using, rtn4 proteins, previous studies, orexinergic neuron, ohcs ), lateral hypothalamus, hypersomnolence complaints
الوصف: A. The white bars indicate the input number of random peptide-displayed phage particles in each round of panning, which is persistently kept at 3x10 9 PFU. The black bars show phage particle titration at the ends of each round before they were amplified for the next round of input. B. Increased phage affinity against IgG OHC and NT1 pooled serum after each round of panning. The binding capacity of enriched phage pools to patient IgGs was measured by phage Elisa. The presenting data are mean ± SD (n = 2).
