دورية
In vivo fluorescence kinetics and localisation of aluminium phthalocyanine disulphonate in an autologous tumour model
| العنوان: | In vivo fluorescence kinetics and localisation of aluminium phthalocyanine disulphonate in an autologous tumour model |
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| المؤلفون: | Witjes, MJH, Speelman, OC, Nikkels, PGJ, Nooren, CAAM, Nauta, JM, van der Holt, B, van Leengoed, HLLM, Star, WM, Roodenburg, JLN |
| المصدر: | British Journal of Cancer; March 1996, Vol. 73 Issue: 5 p573-580, 8p |
| مستخلص: | Sulphonated phthalocyanines are studied as photosensitizers for photodynamic therapy of cancer. Their strong fluorescence and tumour-localising properties make them also potentially useful for detection of cancer by fluorescence. For this purpose, we have studied the fluorescence kinetics and localisation of aluminum phthalocyanine disulphonate (AlPcS2) in 4-nitroquinoline 1-oxide (4NQO)-induced dysplasia and invasive cancer of the oral mucosa of the hard palate in Wistar albino rats. Twenty-two rats were divided into six groups. Five groups were subjected to a 4NQO application period of 8, 12, 16, 20 or 26 weeks and one was a control group. The dysplasia varied from slight to severe and was correlated with the duration of the application period. All animals received a dose of 1 micromol/kg AlPcS2 i.v. Fluorescence images were recorded via a specially designed 'palatoscope' with excitation at 460 +/- 20 nm for autofluorescence, 610 +/- 15 nm for AlPcS2 fluorescence and detection of emission at 675 +/- 15 nm. After subtraction of the two images the specific AlPcS2 fluorescence remained. AlPcS2-mediated fluorescence increased significantly when the severity of dysplasia increased (P<0.04). Also the phenomenon of strong fluorescent spots on the fluorescence images was observed. This always occurred within the first 10 h after injection of AlPcS2. Histological analysis showed a local alteration to a mucosa in 67% of these spots, which was either invasive cancer (29%) or inflammation (38%). These results suggest two different mechanisms of AlPcS2 uptake in tissue, one associated with the presence of generalised dysplasia and another associated with local changes of the epithelial/connective tissue, which is not necessarily specific for tumours. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00070920 15321827 |
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| DOI: | 10.1038/bjc.1996.100 |