دورية
Absence of imprinting of HLA class Ia genes leads to co-expression of biparental alleles on term human trophoblast cells upon IFN-γ induction
| العنوان: | Absence of imprinting of HLA class Ia genes leads to co-expression of biparental alleles on term human trophoblast cells upon IFN-γ induction |
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| المؤلفون: | Lenfant, Françoise, Fort, Marylise, Rodriguez, Anne-Marie, Campan, Agnès, Aguerre-Girr, Maryse, Sommer, Evelyne, Abbal, Michel, Ohayon, Elie, Le Bouteiller, Philippe |
| المصدر: | Immunogenetics; 19980223, Vol. 47 Issue: 4 p297-304, 8p |
| مستخلص: | Abstract: Human trophoblast cells have developed various efficient regulatory mechanisms to prevent cell surface expression of the classical HLA-A, -B, and (but not always) -C class I molecules. This allows them to escape maternal alloimmune attack during pregnancy. However, recent results have demonstrated that such a lack of expression could be reversed in villous cytotrophoblast cells purified from term placenta by in vitro IFN-γ treatment. In this context, we investigated whether both maternal and paternal HLA class Ia antigens were co-dominantly expressed in such trophoblast cells. Using polymerase chain reaction sequence-specific primers for HLA-A and HLA-C alleles, we detected transcripts of both paternal and maternal origins, showing that these genes were not affected by genomic imprinting, at least in term placenta. After in vitro IFN-γ treatment, the polymorphic HLA-A and HLA-B antigens of both parental origins become detectable at the cell surface, as assessed by flow cytometry and/or complement-dependent microtoxicity test. Appearance of paternal antigens on trophoblast cells upon IFN-γ induction raises the question of the in vivo biological consequences of this phenomena, in term of materno-fetal tolerance and in particular of a potential allogeneic cytotoxic immune response. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00937711 14321211 |
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| DOI: | 10.1007/s002510050361 |