دورية أكاديمية
The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
| العنوان: | The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used. |
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| المؤلفون: | Bosseloir, A., Bouzahzah, F., Defrance, T. H., Heinen, Ernst, Simar, L. J. |
| المصدر: | Scandinavian Journal of Immunology, 43 (1), 23-30 (1996) |
| بيانات النشر: | Blackwell Publishing, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Antibiotics, Antineoplastic/pharmacology, Antibodies, Monoclonal/pharmacology, Antigens, CD40/immunology, B-Lymphocytes/immunology, Cell Division, Cell Separation, Cells, Cultured, Child, Child, Preschool, DNA Replication, Dendritic Cells/immunology, Humans, Immunoglobulin G/immunology, Lymphocyte Activation/drug effects, Mitogens/pharmacology, Mitomycin/pharmacology, Palatine Tonsil/cytology, Staphylococcus aureus/immunology, Transferrin/immunology, Life sciences, Anatomy (cytology, histology, embryology...) & physiology, Sciences du vivant, Anatomie (cytologie, histologie, embryologie...) & physiologie |
| الوصف: | Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501Test article |
| اللغة: | English |
| العلاقة: | urn:issn:0300-9475; urn:issn:1365-3083 |
| DOI: | 10.1046/j.1365-3083.1996.d01-7.x |
| الوصول الحر: | https://orbi.uliege.be/handle/2268/23312Test |
| رقم الانضمام: | edsorb.23312 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1046/j.1365-3083.1996.d01-7.x |
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