مورد إلكتروني
Vaccinia Virus Strain MVA Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Glycoprotein Induces Robust Protection and Prevents Brain Infection in Mouse and Hamster Models
| العنوان: | Vaccinia Virus Strain MVA Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Glycoprotein Induces Robust Protection and Prevents Brain Infection in Mouse and Hamster Models |
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| بيانات النشر: | Multidisciplinary Digital Publishing Institute 2023-05-21 |
| تفاصيل مُضافة: | Instituto de Salud Carlos III Agencia Estatal de Investigación (España) European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) San Antonio Medical Foundation Texas Biomedical Forum Lorenzo, Maria M. [0000-0001-7588-673X] Marín-López, A. [0000-0003-2840-1722] Chiem, Kevin [0000-0002-3892-5944] Jiménez-Cabello, Luis [0000-0001-8085-5823] Utrilla-Trigo, S. [0000-0002-7672-7658] Calvo Pinilla, Eva María [0000-0002-4667-4081] Lorenzo, Gema [0000-0003-1869-9051] Moreno, Sandra [0000-0002-3548-037X] Ye, Chengjin [0000-0002-1934-9494] Matía, Alejandro [0000-0002-4246-5135] Brun Torres, Alejandro [0000-0001-7865-538X] Sánchez-Puig Eyre, Juana María [0000-0002-3966-3499] Nogales, Aitor [0000-0002-2424-7900] Mothes, Walther [0000-0002-3367-7240] Uchil, Pradeep D. [0000-0002-7236-858X] Kumar, Priti [0000-0002-6901-5601] Ortego, Javier [0000-0002-4275-7277] Fikrig, Erol [0000-0002-5884-6047] Martínez-Sobrido, Luis [0000-0001-7084-0804] Blasco Lozano, Rafael [0000-0002-8819-5767] Lorenzo, Maria M. Marín-López, A. Chiem, Kevin Jiménez-Cabello, Luis Ullah, Irfan Utrilla-Trigo, S. Calvo Pinilla, Eva María Lorenzo, Gema Moreno, Sandra Ye, Chengjin Park, Jun-Gyu Matía, Alejandro Brun Torres, Alejandro Sánchez-Puig Eyre, Juana María Nogales, Aitor Mothes, Walther Uchil, Pradeep D. Kumar, Priti Ortego, Javier Fikrig, Erol Martínez-Sobrido, Luis Blasco Lozano, Rafael |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The COVID-19 pandemic has underscored the importance of swift responses and the necessity of dependable technologies for vaccine development. Our team previously developed a fast cloning system for the modified vaccinia virus Ankara (MVA) vaccine platform. In this study, we reported on the construction and preclinical testing of a recombinant MVA vaccine obtained using this system. We obtained recombinant MVA expressing the unmodified full-length SARS-CoV-2 spike (S) protein containing the D614G amino-acid substitution (MVA-Sdg) and a version expressing a modified S protein containing amino-acid substitutions designed to stabilize the protein a in a pre-fusion conformation (MVA-Spf). S protein expressed by MVA-Sdg was found to be expressed and was correctly processed and transported to the cell surface, where it efficiently produced cell-cell fusion. Version Spf, however, was not proteolytically processed, and despite being transported to the plasma membrane, it failed to induce cell-cell fusion. We assessed both vaccine candidates in prime-boost regimens in the susceptible transgenic K18-human angiotensin-converting enzyme 2 (K18-hACE2) in mice and in golden Syrian hamsters. Robust immunity and protection from disease was induced with either vaccine in both animal models. Remarkably, the MVA-Spf vaccine candidate produced higher levels of antibodies, a stronger T cell response, and a higher degree of protection from challenge. In addition, the level of SARS-CoV-2 in the brain of MVA-Spf inoculated mice was decreased to undetectable levels. Those results add to our current experience and range of vaccine vectors and technologies for developing a safe and effective COVID-19 vaccine. |
| مصطلحات الفهرس: | COVID-19, SARS-CoV-2, Immunization, Modified vaccinia virus Ankara, Poxvirus, Recombinant viral vectors, Vaccine, artículo |
| URL: | Centro de Investigación en Sanidad Animal (CISA) Publisher's version Sí info:eu-repo/grantAgreement/AEI//PID2021-128466OR-I00 |
| الإتاحة: | Open access content. Open access content https://creativecommons.org/licenses/by/4.0Test openAccess |
| ملاحظة: | English |
| أرقام أخرى: | CTK oai:digital.csic.es:10261/331347 Vaccines 11(5): e1006 (2023) 10.3390/vaccines11051006 2076-393X 37243110 2-s2.0-85160305767 1395213299 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1395213299 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |