مورد إلكتروني
Immunobiology of fibrin-based engineered heart tissue.
| العنوان: | Immunobiology of fibrin-based engineered heart tissue. |
|---|---|
| المؤلفون: | Conradi, Lenard |
| المصدر: | Stem cells translational medicine; vol 4, iss 6, 625-631; 2157-6564 |
| بيانات النشر: | eScholarship, University of California 2015-06-01 |
| تفاصيل مُضافة: | Conradi, Lenard Schmidt, Stephanie Neofytou, Evgenios Deuse, Tobias Peters, Laura Eder, Alexandra Hua, Xiaoqin Hansen, Arne Robbins, Robert C Beygui, Ramin E Reichenspurner, Hermann Eschenhagen, Thomas Schrepfer, Sonja |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | UnlabelledDifferent tissue-engineering approaches have been developed to induce and promote cardiac regeneration; however, the impact of the immune system and its responses to the various scaffold components of the engineered grafts remains unclear. Fibrin-based engineered heart tissue (EHT) was generated from neonatal Lewis (Lew) rat heart cells and transplanted onto the left ventricular surface of three different rat strains: syngeneic Lew, allogeneic Brown Norway, and immunodeficient Rowett Nude rats. Interferon spot frequency assay results showed similar degrees of systemic immune activation in the syngeneic and allogeneic groups, whereas no systemic immune response was detectable in the immunodeficient group (p < .001 vs. syngeneic and allogeneic). Histological analysis revealed much higher local infiltration of CD3- and CD68-positive cells in syngeneic and allogeneic rats than in immunodeficient animals. Enzyme-linked immunospot and immunofluorescence experiments revealed matrix-directed TH1-based rejection in syngeneic recipients without collateral impairment of heart cell survival. Bioluminescence imaging was used for in vivo longitudinal monitoring of transplanted luciferase-positive EHT constructs. Survival was documented in syngeneic and immunodeficient recipients for a period of up to 110 days after transplant, whereas in the allogeneic setting, graft survival was limited to only 14 ± 1 days. EHT strategies using autologous cells are promising approaches for cardiac repair applications. Although fibrin-based scaffold components elicited an immune response in our studies, syngeneic cells carried in the EHT were relatively unaffected.SignificanceAn initial insight into immunological consequences after transplantation of engineered heart tissue was gained through this study. Most important, this study was able to demonstrate cell survival despite rejection of matrix components. Generation of syngeneic human engineered heart tissue, possibly using human i |
| مصطلحات الفهرس: | Myocardium, Th1 Cells, Animals, Rats, Fibrin, Transplantation, Isogeneic, Tissue Engineering, Graft Rejection, Graft Survival, Tissue Scaffolds, Allografts, Bioluminescence imaging, Engineered heart tissue, Immune response, Rat, Rejection, Scaffold, Heart Disease, Cardiovascular, Stem Cell Research, Regenerative Medicine, Bioengineering, 5.2 Cellular and gene therapies, Development of treatments and therapeutic interventions, Biochemistry and Cell Biology, Medical Biotechnology, Clinical Sciences, article |
| URL: | |
| الإتاحة: | Open access content. Open access content public |
| ملاحظة: | application/pdf Stem cells translational medicine vol 4, iss 6, 625-631 2157-6564 |
| أرقام أخرى: | CDLER oai:escholarship.org:ark:/13030/qt1zw499xz qt1zw499xz https://escholarship.org/uc/item/1zw499xzTest https://escholarship.orgTest/ 1378689811 |
| المصدر المساهم: | UC MASS DIGITIZATION From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1378689811 |
| قاعدة البيانات: | OAIster |
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