مورد إلكتروني
A new transcriptome and transcriptome profiling of adult and larval tissue in the box jellyfish Alatina alata: an emerging model for studying venom, vision and sex
| العنوان: | A new transcriptome and transcriptome profiling of adult and larval tissue in the box jellyfish Alatina alata: an emerging model for studying venom, vision and sex |
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| المؤلفون: | Ames, Cheryl Lewis |
| بيانات النشر: | Springer Nature 2016-08-17; College of Computer, Mathematical & Physical Sciences; Digital Repository at the University of Maryland; Biology |
| تفاصيل مُضافة: | Ames, Cheryl Lewis Ryan, Joseph F. Bely, Alexandra E. Cartwright, Paulyn Collins, Allen G. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Cubozoans (box jellyfish) are cnidarians that have evolved a number of distinguishing features. Many cubozoans have a particularly potent sting, effected by stinging structures called nematocysts; cubozoans have well-developed light sensation, possessing both image-forming lens eyes and light-sensitive eye spots; and some cubozoans have complex mating behaviors, including aggregations, copulation and internal fertilization. The cubozoan Alatina alata is emerging as a cnidarian model because it forms predictable monthly nearshore breeding aggregations in tropical to subtropical waters worldwide, making both adult and larval material reliably accessible. To develop resources for A. alata, this study generated a functionally annotated transcriptome of adult and larval tissue, applying preliminary differential expression analyses to identify candidate genes involved in nematogenesis and venom production, vision and extraocular sensory perception, and sexual reproduction, which for brevity we refer to as “venom”, “vision” and “sex”. We assembled a transcriptome de novo from RNA-Seq data pooled from multiple body parts (gastric cirri, ovaries, tentacle (with pedalium base) and rhopalium) of an adult female A. alata medusa and larval planulae. Our transcriptome comprises ~32 K transcripts, after filtering, and provides a basis for analyzing patterns of gene expression in adult and larval box jellyfish tissues. Furthermore, we annotated a large set of candidate genes putatively involved in venom, vision and sex, providing an initial molecular characterization of these complex features in cubozoans. Expression profiles and gene tree reconstruction provided a number of preliminary insights into the putative sites of nematogenesis and venom production, regions of phototransduction activity and fertilization dynamics in A. alata. Our Alatina alata transcriptome significantly adds to the genomic resources for this emerging cubozoan model. This study provides the first annotated |
| مصطلحات الفهرس: | Cubozoa, Pedalium, Sting, Embryo, Gametogenesis, Planulae, Eye, Spawning aggregations, Sperm, Article |
| URL: | College of Computer, Mathematical & Physical Sciences Digital Repository at the University of Maryland Biology University of Maryland (College Park, MD) |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | en_US |
| أرقام أخرى: | UMC oai:drum.lib.umd.edu:1903/27578 Lewis Ames, C., Ryan, J.F., Bely, A.E. et al. A new transcriptome and transcriptome profiling of adult and larval tissue in the box jellyfish Alatina alata: an emerging model for studying venom, vision and sex. BMC Genomics 17, 650 (2016). 1277282831 |
| المصدر المساهم: | UNIV OF MARYLAND, COL PARK From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1277282831 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |