أعرض في EDS

Investigation of putative tumor suppressors on chromosome 16q in nasopharyngeal carcinoma.

التفاصيل البيبلوغرافية
العنوان: Investigation of putative tumor suppressors on chromosome 16q in nasopharyngeal carcinoma.
مرشدي الرسالة: Hui, Wai Ying., Chinese University of Hong Kong Graduate School. Division of Anatomical and Cellular Pathology.
سنة النشر: 2003
المجموعة: The Chinese University of Hong Kong
Original Material: cuhk:324496
مصطلحات موضوعية: Nasopharyngeal Neoplasms, genetics, Chromosomes, Human, Pair 16, Chromosomes, Human, Pair 14, Genes, Tumor Suppressor
الوصف: Hui Wai Ying.
Thesis (M.Phil.)--Chinese University of Hong Kong, 2003.
Includes bibliographical references (leaves 158-189).
in English and Chinese.
Acknowledgements
List of Tables
List of Figures
Table of Contents
Chapter Chapter I: --- Introduction --- p.1
Chapter I. --- Aim of Study --- p.1
Chapter II. --- Literature Review --- p.3
Chapter 1. --- Background --- p.3
Chapter A. --- Epidemiology --- p.3
Chapter B. --- Histopathology --- p.3
Chapter C. --- Etiology --- p.4
Chapter i. --- Environmental Factors --- p.5
Chapter ii. --- Epstein-Barr Virus (EBV) Infection --- p.6
Chapter iii. --- Genetic Factors --- p.9
Chapter 2. --- Molecular Genetics of NPC --- p.11
Chapter A. --- Genome-Wide Studies --- p.11
Chapter i. --- Comparative Genomic Hybridization (CGH) --- p.11
Chapter ii. --- Loss of Heterozygosity (LOH) Studies --- p.12
Chapter iii. --- Homozygous Deletion Study --- p.12
Chapter B. --- NPC-related Oncogenes and Tumor Suppressor Genes --- p.13
Chapter i. --- Oncogenes --- p.13
Chapter ii. --- Tumor Suppressor Genes --- p.14
Chapter 3. --- Chromosome 14q and NPC --- p.19
Chapter A. --- Tumor Suppressor Loci and Cancer-Related Genes on Chromosome14q --- p.20
Chapter i. --- Tumor Suppressor Loci on Chromosome 14q --- p.20
Chapter ii. --- Cancer-Related Genes on Chromosome 14q --- p.26
Chapter 4. --- Chromosome 16q and NPC --- p.28
Chapter A. --- Tumor Suppressor Loci and Candidate Tumor Suppressor genes on Chromosome16q --- p.28
Chapter i. --- Tumor Suppressor Loci on Chromosome 16q --- p.28
Chapter ii. --- Metastasis Suppressor Loci on Chromosome 16q --- p.34
Chapter iii. --- Candidate Tumor Suppressor Genes on Chromosome 16q --- p.34
Chapter Chapter II: --- Materials and Methods --- p.40
Chapter I. --- Cell Lines and Xenografts --- p.40
Chapter 1. --- Cell Lines --- p.40
Chapter 2. --- Xenografts --- p.41
Chapter 3. --- DNA Extraction --- p.42
Chapter II. --- Patients and Biopsy Specimens --- p.44
Chapter 1. --- Manual Microdissection --- p.44
Chapter 2. --- Laser Captured Microdissection (LCM) --- p.46
Chapter 3. --- DNA Extraction --- p.46
Chapter III. --- Comprehensive Screening for Homozygous Deletion Regions on Chromosomes 14q32.12-32.33 and 16q23.1-24.3 in Human Cancers --- p.48
Chapter 1. --- DNA of Human Cancer Cell Lines --- p.48
Chapter 2. --- Sequence-Tagged Sites (STS) Markers --- p.48
Chapter 3. --- Polymerase Chain Reaction (PCR) --- p.49
Chapter IV ---. Investigation of Inactivation of Potential Tumor Suppressor Genes on Chromosome 14q32.12-32.33 and 16q23.1-24.3 --- p.58
Chapter 1. --- Detection of Homozygous Deletion --- p.58
Chapter 2. --- Expression Analysis --- p.58
Chapter A. --- RNA Extraction --- p.58
Chapter B. --- Reverse-Transcription (RT) PCR --- p.61
Chapter i. --- DNase I Digestion --- p.62
Chapter ii. --- First-strand cDNA Synthesis and RNase Digestion --- p.62
Chapter iii. --- Reverse-Transcription (RT)-PCR --- p.63
Chapter C. --- Real-Time RT PCR --- p.63
Chapter 3. --- Methylation Analysis --- p.68
Chapter A. --- Sodium Bisulfite Modification --- p.68
Chapter B. --- Methylation-Specific PCR (MSP) --- p.69
Chapter C. --- Bisulfite Sequencing --- p.70
Chapter D. --- Combined Bisulfite Restriction Analysis (COBRA) --- p.75
Chapter E. --- 5 -aza-2' -deoxycytidine Treatment --- p.76
Chapter ChapterIII： --- Results --- p.78
Chapter I. --- Comprehensive Screening for Homozygous Deletion Regions in Human Cancers --- p.78
Chapter 1. --- Chromosome 14q32.12-3233 --- p.78
Chapter 2. --- Chromosome 16q23.1-243 --- p.79
Chapter II. --- Investigation of Inactivation of Potential Tumor Suppressor Genes in NPC --- p.86
Chapter 1. --- Chromosome 14q --- p.86
Chapter A. --- "WW Domain-Containing Protein, 45-kD （WW45)" --- p.86
Chapter B. --- Apoptosis Stimulating Protein of p53（ASPP1) --- p.88
Chapter 2. --- Chromosome 16q --- p.92
Chapter A. --- WW Domain-Containing Oxidoreductase （WWOX) --- p.92
Chapter i. --- Homozygous Deletion Screening of WWOX --- p.92
Chapter ii. --- Expression of Aberrant Splicing Transcripts of WWOX in NPC --- p.94
Chapter iii. --- Sequencing of WWOX Aberrant Transcripts --- p.95
Chapter iv. --- Quantitative Analysis of WWOX Transcripts in NPC --- p.95
Chapter v. --- Methylation Analysis --- p.99
Chapter B. --- H-Cadherin (CDH13) --- p.102
Chapter i. --- Analysis of H-cadherin Deletion on Cancer Cell Lines and Xenografts --- p.102
Chapter ii. --- Expression Analysis of H-Cadherin by RT-PCR and Real-Time RT-PCR --- p.102
Chapter iii. --- Analysis of Promoter Hypermethylation by Methylation-Specific PCR (MSP) and Bisulfite Sequencing in NPC Cell Lines and Xenografts --- p.104
Chapter iv. --- Demethylation Study of H-Cadherin in C666-1 Cell Line --- p.105
Chapter v. --- Methylation Analysis of H-Cadherin in Primary Tumors --- p.105
Chapter vi. --- Methylation Analysis of H-Cadherin in Human Cancer Cell Lines --- p.106
Chapter C. --- Myeloid Translocation Gene on Chromosome 16 (MTG16) --- p.113
Chapter i. --- Deletion Analysis of MTG16 in Cancer Cell Lines and Xenografts --- p.113
Chapter ii. --- Differential Expression of MTG16a and MTG16b Transcripts in NPC Cell Lines and Xenografts --- p.113
Chapter iii. --- Methylation Analysis of MTG16b in NPC Cell Lines and Xenografts --- p.118
Chapter iv. --- Sequencing of MTGl 6b RT-PCR Products --- p.119
Chapter v. --- Demethylation Study of MTG16b in HK-1 Cell Line --- p.119
Chapter vi. --- Promoter Methylation Analysis of MTG16b by MSP in Primary NPC and Cancer Cell Lines --- p.120
Chapter Chapter IV: --- Discussion --- p.124
Chapter I. --- Comprehensive Homozygous Deletion Screening of Chromosomes 14q32.12-32.33 and 16q23.1-24.3 in Human Cancer Cell Lines and Xenografts --- p.124
Chapter II. --- Investigation of Candidate Tumor Suppressor Genes on Chromosome 14q in NPC --- p.128
Chapter III. --- Alterations of Candidate Tumor Suppressor Genes on Chromosome 16q in NPC --- p.133
Chapter 1. --- Expression of Aberrant Transcripts of WWOX in NPC --- p.133
Chapter 2. --- Methylation-Associated Silencing of H-Cadherin and MTG16b in NPC --- p.140
Chapter Chapter V: --- Conclusion --- p.154
Chapter Chapter VI: --- References --- p.158
Original Identifier: oai:cuhk-dr:cuhk_324496
نوع الوثيقة: Bibliography
وصف الملف: print; 189 leaves : ill. (some col.) ; 30 cm.
اللغة: Chinese
English
الإتاحة: http://library.cuhk.edu.hk/record=b5891615Test
http://repository.lib.cuhk.edu.hk/en/item/cuhk-324496Test
حقوق: Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0Test/)
رقم الانضمام: edsndl.cuhk.edu.hk.oai.cuhk.dr.cuhk.324496
قاعدة البيانات: Networked Digital Library of Theses & Dissertations
الوصف
الوصف غير متاح.