رسالة جامعية
Eficácia da interleucina-12 (il-12) no tratamento adjuvante da tricosporonose murina experimental e caracterização in vitro de fatores associados à virulência em trichosporon spp
| العنوان: | Eficácia da interleucina-12 (il-12) no tratamento adjuvante da tricosporonose murina experimental e caracterização in vitro de fatores associados à virulência em trichosporon spp |
|---|---|
| المؤلفون: | Bentubo, Henri Donnarumma Levy UNIFESP |
| المساهمون: | Gompertz, Olga Fischman Gompertz UNIFESP, Universidade Federal de São Paulo (UNIFESP) |
| بيانات النشر: | Universidade Federal de São Paulo (UNIFESP) |
| سنة النشر: | 2014 |
| المجموعة: | Universidade Federal de São Paulo (UNIFESP): Repositório Institucional |
| مصطلحات موضوعية: | tricosporonose experimental murina, interleucina-12 |
| الوصف: | Trichosporon yeasts have been associated with invasive and severe opportunistic infections in immunocompromised patients. In spite of recent scientific and technological advances, are still frequent reports of treatment failure and mortality rates are quite high. The concern of the scientific community with the patient of the new century stimulated the search for more effective methods of diagnosis and new therapeutic regimens. The objective of this research was to evaluate the efficacy of interleukin -12 as an adjuvant of voriconazole in the treatment of experimental murine trichosporonosis; verify the sensitivity to antifungal agents by comparing the E-test™ with the microdilution method, to investigate the influence that the incubation temperature over the expression of proteasis , phospholipasis , lipasis and DNasis in Trichosporon species through the use of specific substrates , following the classical recommendations in the literature and characterize the production of polymers compatible with melanin in the cell wall of Trichosporon spp. by growing isolates on media with different sources of diphenols . The use of IL -12 in the adjuvant treatment of experimental murine trichosporonosis highest efficiency in controlling the growth of the fungus and minimized general clinical signs of BALB/c mice immunosuppressed and inoculated with standard strain of T. asahii (CBS 2479). We conclude that IL - 12 is an effective alternative for the treatment of trichosporonosis and future studies will evaluate its use in humans. Elevated MICs for fluconazole were observed in sensitivity tests with eminent discriminatory capacity of the E-test™ in identifying isolates with resistance trend. Comparative tests for MICs presented by E-test™ and microdilution indicated a low correlation between the methods. Lipases were the main enzymes produced by Trichosporon spp. and its activity showed enhanced at 25 °C, although all enzymes studied have been detected , albeit at varying intensities. Although the enzymatic expression was ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | 196 p. |
| اللغة: | Portuguese |
| العلاقة: | https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=771228Test; BENTUBO, Henri Donnarumma Levy. Eficácia da interleucina-12 (il-12) no tratamento adjuvante da tricosporonose murina experimental e caracterização in vitro de fatores associados à virulência em trichosporon spp. 2014. 196 f. Tese (Doutorado) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2014.; http://repositorio.unifesp.br/handle/11600/49012Test; 2014-0692.pdf |
| الإتاحة: | http://repositorio.unifesp.br/handle/11600/49012Test https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=771228Test |
| حقوق: | Acesso restrito |
| رقم الانضمام: | edsbas.722D759A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |