رسالة جامعية
Multimodal imaging probes and delivery systems for cancer nanomedicine
| العنوان: | Multimodal imaging probes and delivery systems for cancer nanomedicine |
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| المؤلفون: | Prabhakar, Neeraj |
| المساهمون: | Professor Matthias Nees, SRH Fernhochschule, Germany, Professor Jessica M. Rosenholm, Åbo Akademi University, Finland, Professor Pekka E. Hänninen, University of Turku, Finland, Åbo Akademi University, Faculty of Science and Engineering, Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering |
| بيانات النشر: | Åbo Akademi - Åbo Akademi University |
| سنة النشر: | 2018 |
| المجموعة: | Doria (National Library of Finland / |
| مصطلحات موضوعية: | 221 Nanoteknology, 317 Pharmacy, Pharmacy, Farmasi |
| الوصف: | Nanoparticles have emerged as one of the most promising tools for addressing central challenges in cancer diagnostics and therapy. This thesis presents the design, surface functionalization, biocompatibility, intracellular interactions and applicability of nanoparticles as potential tools for cancer diagnosis and RNAi therapeutics. The thesis is divided into two parts; 1) cancer cell imaging and 2) siRNA delivery. In part 1, studies were performed with inherently fluorescent carbon-based nanoparticles (nanodiamonds, NDs, and nanographene oxide, nGO) in order to evaluate their suitability for cancer cell imaging (in vivo and in vitro). A novel application of NDs in super resolution correlative light and electron microscopy is presented here, whereby NDs are used as a dual-purpose fluorescent and electron dense probes for correlative multi-modal microscopy. Further, the intracellular interactions of NDs are studied to understand the biocompatibility of nondegradable NDs and the reasons underlying the biocompatibility. Moreover, this thesis elucidates the role of organic surface modifications for enhancing the optical properties of nanographene oxide (nGO) for in vivo imaging. When nGO was surface functionalized with the organic polymer PEG-PEI (polyethyleneimine – a polyethylene glycol co-polymer), and attached to the cancer cell affinity ligand FA (folic acid), its dispersibility, cellular internalization and quantum efficiency were improved compared to the non-modified nGO. The surface functionalized nGOs were further applied as optical markers for the non-invasive in vivo imaging of cancer cells in a model organism. The nGOs were found to be well suited for detecting cancer cells over the studied 1-week period. In part 2, systems for the efficient delivery of therapeutic cargo to cancer cells were studied using a nanodiamond (ND) - silica (MSN) composite (ND@MSN) and mesoporous silica nanoparticles (MSN) with redox responsive linkers. ND@MSN, a novel composite material was synthesized by taking advantage of the ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| ردمك: | 978-952-12-3674-7 978-952-12-3675-4 952-12-3674-4 952-12-3675-2 |
| العلاقة: | http://www.doria.fi/handle/10024/149454Test; URN:ISBN:978-952-12-3675-4 |
| الإتاحة: | http://www.doria.fi/handle/10024/149454Test |
| حقوق: | This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. ; Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. ; Publikationen är skyddad av upphovsrätten. Den får läsas och skrivas ut för personligt bruk. Användning i kommersiellt syfte är förbjuden. |
| رقم الانضمام: | edsbas.D8E9721F |
| قاعدة البيانات: | BASE |
| ردمك: | 9789521236747 9789521236754 9521236744 9521236752 |
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