رسالة جامعية
The functions of growth factors in pituitary tumors and their effect on IGF1 receptor regulation ; Die Funktionen von Wachstumsfaktoren in Hypophysentumoren und ihr Einfluss auf die Regulierung des IGF1 Rezeptors
| العنوان: | The functions of growth factors in pituitary tumors and their effect on IGF1 receptor regulation ; Die Funktionen von Wachstumsfaktoren in Hypophysentumoren und ihr Einfluss auf die Regulierung des IGF1 Rezeptors |
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| المؤلفون: | Drost, Adriana |
| المساهمون: | n, Prof. Dr. Hans-Jürgen Quabbe, Prof. Dr. Annette Grüters-Kieslich |
| سنة النشر: | 2002 |
| المجموعة: | FU Berlin: Refubium |
| مصطلحات موضوعية: | Pituitary adenoma, GH3 cells, growth factors, cytokines, apoptosis, IGF1 receptor, ddc:610 |
| الوصف: | The functions of growth factors in pituitary tumors and their effect on IGF1 receptor regulation TITLE AND CONTENTS 1\. INTRODUCTION 14 1.1. Pituitary tumor etiology 1.2. Growth factor actions in the normal and tumorous pituitary 1.3. Primary aim of the project 1.4. Structure and function of IGF1 and its receptor 1.5. Secondary aim of the project 2\. METHODS 18 2.1.Patient Information 2.2. Tumor preparation 2.3. Cell culture 2.4. Bioassays 2.5. Hormone measurements 2.6. Flow cytometry 2.7. I-IGF1 competitive binding assay 3\. RESULTS 30 3.1. Assay Optimization 3.2. Effect of growth factors on H-thymidine incorporation, apoptosis and hormone secretion in GH cells 3.3. Detection of growth and secretion promoting activities in human pituitary tumor fractions 3.4. Functional effects of growth factors on primary human pituitary cells 3.5. IGF1R studies in PP 4\. DISCUSSION 72 4.1. Major aims and results 4.2. Discussion of effects of growth factors on proliferation and apoptosis in pituitary tumor cells 4.3. Discussion of effects of growth factors on hormone secretion in pituitary tumor cells 4.4. Discussion of the demonstration of growth factor-like activities in human pituitary tumors 4.5. Discussion of findings on human pituitary tumor fractions as related to clinical and histological diagnoses 4.6. Comparison of apoptosis induction in GH cells and PP by serum deprivation 4.7. Discussion of the new method to detect cell surface IGF1 receptors by flow cytometry 4.8. Discussion of IGF1R regulation in PP 5\. SUMMARY 85 6\. REFERENCES 87 ; The functions of growth factors in pituitary tumors and their effect on IGF1 receptor regulation This study examined the role of growth factors and one of their receptors in pituitary tumor growth, as assessed in a rat pituitary cells line (GH3 cells) and/or in primary human pituitary adenoma cells (PP). Tthe following findings were revealed: · 3H-thymidine incorporation of GH3 cells was decreased by bFGF, IGF1 and IL2, and increased by EGF and IL6, suggesting a role of these growth ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://refubium.fu-berlin.de/handle/fub188/10736Test; http://dx.doi.org/10.17169/refubium-14934Test; urn:nbn:de:kobv:188-2002002868 |
| DOI: | 10.17169/refubium-14934 |
| الإتاحة: | https://doi.org/10.17169/refubium-14934Test https://refubium.fu-berlin.de/handle/fub188/10736Test https://nbn-resolving.org/urn:nbn:de:kobv:188-2002002868Test |
| حقوق: | http://www.fu-berlin.de/sites/refubium/rechtliches/NutzungsbedingungenTest |
| رقم الانضمام: | edsbas.83406027 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17169/refubium-14934 |
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