رسالة جامعية
EQUINE LEPTOSPIROSIS: A STUDY OF LEPTOSPIRA INTERROGANS SEROVAR BRATISLAVA PATHOGENICITY IN HORSES AND DEVELOPMENT OF A DIAGNOSTIC TEST USING REVERSE VACCINOLOGY
| العنوان: | EQUINE LEPTOSPIROSIS: A STUDY OF LEPTOSPIRA INTERROGANS SEROVAR BRATISLAVA PATHOGENICITY IN HORSES AND DEVELOPMENT OF A DIAGNOSTIC TEST USING REVERSE VACCINOLOGY |
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| المؤلفون: | Zilch, Tiago Jaquel |
| المساهمون: | Chang, Yung-Fu, McDonough, Sean, Mohammed, Hussni |
| سنة النشر: | 2020 |
| المجموعة: | Cornell University: eCommons@Cornell |
| مصطلحات موضوعية: | Diagnostic test, ELISA, Equine leptospirosis, Leptospirosis, Reverse vaccinology |
| الوصف: | 115 pages ; Supplemental file(s) description: Supplementary Material S3: Protein data from antigenic selection approach and BLAST results., Supplementary Material S2: Histopathological photomicrographs., Supplementary Material S1: Excel spreadsheet with supplementary information from Chapter 2. This file contains: Temperature and Clinical chemistries at the individual level, and pangenomic analysis data. ; Leptospirosis is a neglected worldwide zoonotic disease caused by pathogenic gram-negative spirochetes of the genus Leptospira. It can be life threatening for animals and humans. Leptospira can cause livestock losses among pigs, cattle, horses, goats and sheep, thereby inflicting significant losses on the livestock agronomy. This is the first time an analysis using L. interrogans serovar Bratislava, which is believed to be pathogenic for horses, is presented. Six young female foals were challenged with 1 x 109 L. interrogans serovar Bratislava strain PigK151 and observed over a period of 30 days. Temperature, blood and urine, as well as post-mortem samples were evaluated. No pyrexia was noted. PCR was negative from all plasma, urine and tissue samples. No Leptospires were recovered from either plasma or urine culture. All challenged foals developed antibodies against L. interrogans serovar Bratislava as determined by the gold standard microscopic agglutination test (MAT), beginning on day 3 until the last day of the study while all the control animals remained serologically negative. Cross-reactivity on the MAT test results and DNA analysis helped to formulate a reasonable answer for the question about L. interrogans serovar Bratislava pathogenicity in horses. Cross-reactivity among serovars used on the MAT test shows that a better diagnostic test is necessary. Reverse vaccinology has been broadly used to screen for surface-exposed proteins and antigens of important pathogens, like outer membrane proteins and extracellular proteins used as potential vaccine candidates. In order to develop a test that can serve ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf; application/vnd.ms-excel |
| اللغة: | English |
| العلاقة: | Zilch_cornell_0058O_10862; http://dissertations.umi.com/cornell:10862Test; https://hdl.handle.net/1813/70263Test; https://doi.org/10.7298/xdh6-z694Test |
| DOI: | 10.7298/xdh6-z694 |
| الإتاحة: | https://doi.org/10.7298/xdh6-z694Test https://hdl.handle.net/1813/70263Test http://dissertations.umi.com/cornell:10862Test |
| رقم الانضمام: | edsbas.5778DD2C |
| قاعدة البيانات: | BASE |
| DOI: | 10.7298/xdh6-z694 |
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