مؤتمر
Characterization of Arabidopsis GLN1;5 knockout mutant
| العنوان: | Characterization of Arabidopsis GLN1;5 knockout mutant |
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| المؤلفون: | Ćuković, Katarina, Dragićević, Milan, Zdravković-Korać, Snežana, Simonović, Ana, Bogdanović, Milica, Todorović, Slađana |
| المساهمون: | Uzelac, Branka |
| المصدر: | 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade |
| بيانات النشر: | Serbian Plant Physiology Society |
| سنة النشر: | 2018 |
| المجموعة: | RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" / РАДаР - Репозиторијум Архивираних Дигиталних Радова Институт за биолошка истраживања "Синиша Станковић" |
| مصطلحات موضوعية: | Arabidopsis, glutamine synthetase, knockout mutant, phenotype, germination |
| الوصف: | Glutamine synthetase is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis genome encodes one chloroplastic (GLN2) and five cytosolic isoforms, GLN1;1 through GLN1;5, with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remained unknown. To elucidate the GLN1;5 function, we have studied a GLN1;5 knockout line (GLN1;5KO), homozygous for T-DNA insertion within the GLN1;5. The GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds is 73.3% of WT seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% mutant seeds germinated in water within 10 days, in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in increasing NH4Cl concentrations potentiates the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation within the seed, as well as assimilation and/or detoxification of ammonium from the environment. ; Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 18. |
| نوع الوثيقة: | conference object |
| اللغة: | English |
| ردمك: | 978-86-912591-4-3 86-912591-4-0 |
| العلاقة: | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS//; https://radar.ibiss.bg.ac.rs/handle/123456789/4333Test; 264415756; https://radar.ibiss.bg.ac.rs/bitstream/id/8838/3-IntConfPlantBiology_2018-18.pdfTest; https://hdl.handle.net/21.15107/rcub_ibiss_4333Test |
| الإتاحة: | https://radar.ibiss.bg.ac.rs/handle/123456789/4333Test https://radar.ibiss.bg.ac.rs/bitstream/id/8838/3-IntConfPlantBiology_2018-18.pdfTest https://hdl.handle.net/21.15107/rcub_ibiss_4333Test |
| حقوق: | openAccess ; https://creativecommons.org/licenses/by/4.0Test/ ; ARR ; © 2018 by the Serbian Plant Physiology Society |
| رقم الانضمام: | edsbas.41040236 |
| قاعدة البيانات: | BASE |
| ردمك: | 9788691259143 8691259140 |
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