كتاب
Immunolocalization and RT-PCR for the detection and quantification of growth factor and receptor gene expressi•on
| العنوان: | Immunolocalization and RT-PCR for the detection and quantification of growth factor and receptor gene expressi•on |
|---|---|
| المؤلفون: | Myers, Simon R, Navsaria, Harshad A |
| المصدر: | Growth Factors and Receptors ; page 149-174 ; ISBN 9780199636471 9781383049305 |
| بيانات النشر: | Oxford University PressOxford |
| سنة النشر: | 1998 |
| الوصف: | In many research fields that impact directly on clinical medicine (e.g. wound healing) a new emphasis on the examination of human tissue has emerged facilitated by the availability of more sophisticated laboratory techniques. This immediately defines the limiting factor in choosing an assay as the volume of human tissue available. To investigate human growth factor and re ceptor gene expression in any depth, assessment of both protein and messenger RNA (mRNA) for ligand and receptor must be made, in terms both of quantity and of distribution, on small tissue samples. 6 mm punch biopsy wounds of skin for example can be sutured directly, or converted into a small ellipse. In the former case the sutures tend to pull out to leave a pale, circular scar, and in the latter a linear scar of< 1 cm length remains. A 6 mm punch biopsy of skin provides around 60 mg wet weight of tissue. For protocols that require repeated biopsy of an individual, 3 mm punch biopsies are preferable. These can be left to heal by secondary intention, leaving a minimal ‘pit’ scar. Note: researchers must meet all local and/or national ethical requirements before taking biopsies for experimental purposes from human subjects. |
| نوع الوثيقة: | book part |
| اللغة: | unknown |
| ردمك: | 978-0-19-963647-1 978-1-383-04930-5 0-19-963647-8 1-383-04930-0 |
| DOI: | 10.1093/oso/9780199636471.003.0007 |
| الإتاحة: | https://doi.org/10.1093/oso/9780199636471.003.0007Test https://academic.oup.com/book/chapter-pdf/52545615/isbn-9780199636471-book-part-7.pdfTest |
| رقم الانضمام: | edsbas.E78D8946 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780199636471 9781383049305 0199636478 1383049300 |
|---|---|
| DOI: | 10.1093/oso/9780199636471.003.0007 |