كتاب
CUT&RUN Profiling of the Budding Yeast Epigenome
| العنوان: | CUT&RUN Profiling of the Budding Yeast Epigenome |
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| المؤلفون: | Brahma, Sandipan, Henikoff, Steven |
| المصدر: | Methods in Molecular Biology ; Yeast Functional Genomics ; page 129-147 ; ISSN 1064-3745 1940-6029 ; ISBN 9781071622568 9781071622575 |
| بيانات النشر: | Springer US |
| سنة النشر: | 2022 |
| الوصف: | Mapping the epigenome is key to describe the relationship between chromatin landscapes and the control of DNA-based cellular processes such as transcription. Cleavage under targets and release using nuclease (CUT&RUN) is an in situ chromatin profiling strategy in which controlled cleavage by antibody-targeted Micrococcal Nuclease solubilizes specific protein-DNA complexes for paired-end DNA sequencing. When applied to budding yeast, CUT&RUN profiling yields precise genome-wide maps of histone modifications, histone variants, transcription factors, and ATP-dependent chromatin remodelers, while avoiding cross-linking and solubilization issues associated with the most commonly used chromatin profiling technique Chromatin Immunoprecipitation (ChIP). Furthermore, targeted chromatin complexes cleanly released by CUT&RUN can be used as input for a subsequent native immunoprecipitation step (CUT&RUN.ChIP) to simultaneously map two epitopes in single molecules genome-wide. The intrinsically low background and high resolution of CUT&RUN and CUT&RUN.ChIP allows for identification of transient genomic features such as dynamic nucleosome-remodeling intermediates. Starting from cells, one can perform CUT&RUN or CUT&RUN.ChIP and obtain purified DNA for sequencing library preparation in 2 days. |
| نوع الوثيقة: | book part |
| اللغة: | unknown |
| ردمك: | 978-1-07-162256-8 978-1-07-162257-5 1-07-162256-0 1-07-162257-9 |
| DOI: | 10.1007/978-1-0716-2257-5_9 |
| الإتاحة: | https://doi.org/10.1007/978-1-0716-2257-5_9Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.3BC98FFE |
| قاعدة البيانات: | BASE |
| ردمك: | 9781071622568 9781071622575 1071622560 1071622579 |
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| DOI: | 10.1007/978-1-0716-2257-5_9 |